Multi-Channel Voltage / Current Clamp EVC4000 EVC4000-4 (complete four-channel system) EPITHELIAL PHYSIOLOGY More channels and a wider range of voltage clamp commands than WPI’s classic DVC-1000. The superior design of the cartridge electrodes makes 100-volt current excursion unnecessary, so this safe, low-voltage system is easier to adjust and use. EVC4000 employs the voltage clamp technique to monitor membrane permeability as a function of membrane voltage or applied chemicals. When combined with WPI’s patented EKC and EKV cartridge electrodes, EVC4000 can efficiently voltage or current clamp up to four sample membranes simultaneously using safe moderate voltages on the current wire leads. The superior design of the cartridge electrodes makes 100-volt current excursion unnecessary, so this safe, lowvoltage system is easier to adjust and use. Extremely stable and accurate, each module, with its companion preamplifier, can operate independently in one of three different modes: Voltage Clamp (VC), Current Clamp (CC), or Open Circuit Potential (PD) measurement. EVC4000 can be controlled from the front panel of the instrument or from computer generated commands applied at the rear panel of the instrument. A feature unique to EVC4000 is an electronic potentiostat in the preamplifier box that maintains the serosal electrode invariant potential at zero relative to system ground. The preamplifier apparatus actively maintains one surface of the test membrane close to ground potential under all operating conditions. EVC4000 SPECIFICATIONS PREAMPLIFIER Input Resistance Input Leakage Current Maximum Input Voltage VOLTAGE CLAMP Panel Display Clamp Voltage / External Input Range of Voltage Electrodes Max. Clamp Voltage Fluid Resistance Compensation CURRENT CLAMP Panel Display Maximum Clamp Current Current Clamp Output DISPLAY RESOLUTION Voltage Current DIMENSIONS SHIPPING WEIGHT (EVC4000-4) 1012 Ohms 100 pA, max. ±15 volts ±200 mV ±0.1 mV 100 mV per Volt ±32 Volts ±100 mV 0 to 1000 Ohms ±999 µA ±1 µA ±1 milliampere 1 µA / mV 0.1 mV 1 µA 18.25 x 7.2 x 9.6 in. (46 x 18 x 24 cm) 26 lb (12 kg) References W. K. MacNaughton (2000) “Role of constitutive cyclooxygenase-2 in prostaglandin-dependent secretion in mouse colon in vitro.” Journal of Pharmacology and experimental Therapeutics 293, 2, 539-544 EVC4000-4 EVC4000-3 EVC4000-2 EVC4000-1 4-Channel Voltage Clamp & preamps (shown above) 3-Channel Voltage Clamp & preamps 2-Channel Voltage Clamp & preamps 1-Channel Voltage Clamp & preamp Specify line voltage OPTIONAL ACCESSORIES SYS-EVC4000 Replacement Voltage Clamp & EVC3 Preamplifier EVC3 Replacement Preamplifier Module EK1 Ussing Electrode Kit (2 voltage, 2 current) EKV Extra Ussing Voltage Electrode (each) EKC Extra Ussing Current Electrode (each) 2851 BNC Cable 3485 Post Mounting Kit for Preamp 86 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com