ISO-H2S-2 ISO-H2S-100-Cxx Although hydrogen sulfide (H2S) is generally thought of as a poisonous gas, it is endogenously produced in many mammalian tissues. It has been detected in micromolar amounts in blood and brain tissue. Hydrogen sulfide is reported as having a broad range of biological functions and although its potential to participate in cell signaling is clear, this biological role is not well understood. H2S is strongly anagolous to nitric oxide (NO) because they share several physical and metabolic properties. Like NO, H2S is a potent vascular signal that can mediate vasoconstriction or vasorelaxation depending on the O2 level and tissue. In the rat aorta, H2S concentrations that mediate rapid constriction at one O2 level will cause rapid relaxation at lower O2 levels. The ISO-H2S sensor is a low detection limit sensor to record H2S in vitro. It is the only sensor available that measures H2S. 5000 pA removal of H2S 20000 2 min 18000 16000 14000 12000 10000 8000 6000 4000 2000 0 0 2000 y = 2.2596x + 154.29 R2 = 0.9963 Current / pA Current (pA) 500 pA 1 min 8000 10000 4000 6000 Concentration/nM AA DA NO2+ NO3- Glu H2S H2S additions Time / s Time (s) The stepped response to increasing concentrations of H2S are linear (see inset, R=.9963). The sensor is insensitive to competing species such as ascorbic acid, dopamine, nitrate, nitrite, and glutathials. ISO-H2S-2 ISO-H2S-100-Cxx 600016 100084 600015 2 mm Shielded Hydrogen Sulfide Macro Sensor Hydrogen Sulfide Micro Sensor (pkg of 2) Replacement Sleeves for ISO-H2S-2 (pkg of 4) Filling Solution for ISO-H2S-2 Start-up Kit for ISO-H2S-2 BIOSENSING ISO-COP-2 SPECIFICATIONS Sensor diameter ...............................................2mm Sensor sensitivity ..................................~0.5 pA/nM Detection limit .............................................. ~10nM Linear range ........................................10nM–10µM Response time .................................. <10 seconds ISO-COP-2 Electrochemical CO Sensor for In Vivo Measurements for CO dissolved in solution Carbon monoxide (CO) is a versatile mediator of physiological processes. Carbon monoxide (CO) formed by internal mechanisms (endogenous) is measured in a variety of ways, but standard measurement methods are of limited utility in most biological systems. WPI's ingenious ISO-COP-2 CO sensor measures CO in vivo or in vitro in real time! This CO sensor is comprised of a 2.0mm stainless steel body with a replaceable membrane-covered sleeve. The sleeve is filled with electrolyte. It is an amperometric sensor designed for use in cell culture and similar applications. In principle, CO diffuses through the gas-permeable membrane and is then oxidized to CO2 on the working electrode of the sensor. This oxidation creates a current with a magnitude directly related to the concentration of CO in solution. It is designed for use with WPI's TRB4100 (4-Channel) or TBR1025 (1-Channel) Free Radical Analyzers. References Motterlini, M., Sawle, P., Bains, S., Hammad, J., Alberto, R., Foresti, R. and Green, C, "CORM-A1: A new pharmacologically active carbon monoxide-releasing molecule," FASEB Journal, November 19, 2004, express article 10.1096/fj.04-2169fje. ISO-COP-2 95620 95611 95699 2 mm Shielded Carbon Monoxide Sensor Replacement Sleeves (pkg of 4) Filling Solution Start-up Kit GLUCOSE SENSOR SPECIFICATIONS IN VITRO PRECISION GLUCOSE RANGE RESPONSE TIME (sec) IN VIVO CALIBRATION Coefficient of Variation (CV) ≤5% 36 - 450 mg/dl (or 2-25 mM/L) 100 - 300s In vivo calibration Acetaminophen, ascorbic acid, uric acid 5 cm 0.6 × 0.7 mm Ag/AgCl 0.65 -0.7V vs. Ag/AgCl 3-4 months in solutions at room temperature under continuous polarization; 15-30 days in vivo 6 months 20° to 40° C (68° to 104° F) 10° to 25° C (50° to 77° F) IGS100 – Implantable glucose sensor Measuring glucose in vivo over the long term is challenging and difficult. Previous measurement systems were limited to acute studies or a few days at best. WPI introduces a new kind of implantable glucose sensor based on a patented technology. This sensor provides a tool for researchers to directly detect glucose in chronic studies in vitro or in vivo. The sensor is fully compatible with WPI’s Apollo system. IGS100 91580 Implantable Glucose Sensor (pkg of 2) Microsensor Adapter Cable INTERFERENCE SPECIES LENGTH SENSOR SIZE REFERENCE ELECTRODe POLARIZATION VOLTAGE (V) SENSOR LIFE SHELF LIFE OPERATION CONDITIONS STORAGE CONDITIONS UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 55