Mayagüez Bay CDOM 0.09 Absorption [1/m] 0.07 0.05 0.03 0.01 -0.01 380 480 580 Wavelength [nm] 680 Mayagüez Bay “Determining CDOM Absorption Spectra in Diverse Coastal Environments Using a Multiple Pathlength, Liquid Core Waveguide System”, Continental Shelf Research, July 2002, 22:9, p 1301-1310. “System Analyzes Water Samples at Sea”, NASA Aerospace Technology Innovation, 2001, 9 (5). http://nctn.hq.nasa.gov/innovation/innovation95/3techtrans2.html R. L. Miller and E. D’Sa. “Evaluating the influence of CDOM on the remote sensing signal in the Mississippi River Bight”. In Eos Transactions AGU Ocean Sciences, 2002. Honolulu, HI, p. 171. E. D’Sa, R.L. Miller and R. Trzaska. “Aparent Optical Properties in Waters Influenced by the Mississippi River”, Proceedings of the Seventh Thematic Conference, Remote Sensing for Marine and Coastal Environments, 2002, 6 pg, Miami, FL. R. L. Miller, C. Hall, C. Del Castillo, B. McKee and M. Dagg. “Bio-optical Properties of the Mississippi River Plume and Adjacent Shelf.” ASLO Aquatic Sciences, Albuquerque, NM, 2001. R. L. Miller, M. Belz and S. Y. Liu, “Measuring the absorption of CDOM in the field using a multiple pathlength liquid waveguide system”, Ocean Optics XV, paper 1308, Monaco, October 2000. Fig. 2 — CDOM Sample “Mayagüez Bay” was collected from the high salinity oligotrophic waters of Mayagüez Bay on the west coast of Puerto Rico (2001). Data courtesy of NASA Stennis Space Center. A standard PC or laptop (not included) is connected to the TIDAS E via a RJ-45 Ethernet interface. For spectrometer requirements and software options, see TIDAS-E. Absorbance 0.025 0.02 0.015 0.01 0.005 0 -0.005 Ponceau S measured with 200 cm pathlength baseline 0.7 nM 1.8 nM 3 nM 125 100 75 50 25 0 -25 µAU/cm Mobility The system is designed for mobility. The components of the UltraPath system are designed to function over a broad range of laboratory and field environments. Samples Two typical absorption spectra recorded with an UltraPath (UPUV) of a seawater and a fresh water sample collected in November 2007 are shown in Fig. 1. Due to their high absorbance, both samples were analyzed in the 10 cm pathlength. The CDOM sample labeled Mayagüez Bay in Fig. 2 is from oligotrophic, low productive waters with high salinity collected off the west coast of Puerto Rico in the Mayagüez Bay. Special attention should be drawn to the exceptional sensitivity of UltraPath enabling detection of CDOM absorption below 0.03 m-1. To exemplify the performance of the UltraPath in Laboratory Chemistry and Process Control, Ponceau S absorbance was measured with the 200 cm pathlength of an UltraPath. Normalizing the Ponceau absorbance graph to AU/cm, the range of this measurement is 150 μAU with a noise level below 2 μAU peak to peak. Sub-nanomolar concentration of this dye can clearly and reliably be detected, which is a novelty in absorbance based spectroscopy. 400 450 500 550 600 650 700 Wavelength [nm] Fig. 3 – Ponceau S absorption measured with UltraPath (200 cm cell). Ponceau S was dissolved in Millipore water. SPEC TROS CO PY UltraPath Specifications DYNAMIC RANGE WAVELENGTH RANGE 5 μAU/cm to 1 AU/cm 0.002 m-1 to 200 m-1 250 nm – 730 nm (UPUV) 380 nm – 730 nm (UPVIS) < 0.2 mAU < 1 mAU/h 2, 10, 50 & 200 cm (user selectable) 2 mm 10 mL (at 200 cm pathlength) 1/8” 600 μm Most organic and inorganic solvents UPUV: 44 lb (20 kg) UPVIS: 33 lb (15 kg) WAVELENGTH RESOLUTION (FWHM) 5 nm NOISE (PEAK TO PEAK) DRIFT OPTICAL PATHLENGTH SAMPLE CELL INNER DIAMETER CELL VOLUME SAMPLE INLET / OUTLET FIBER INPUT/OUTPUT SOLVENT RESISTANCE SHIPPING WEIGHT Particulate Absorption Particulate absorption can be measured by the well established Quantitative Filter Technique (QFT). WPI now offers a fiber optic filter holder for Glass Fiber Filters (QFT1, page 206) which can be used with the spectrometer (TIDAS E) and light source (D4H or FO6000) supplied with the UltraPath. With this accessory, particulate absorption can be measured on site, avoiding loss of spectral information due to freezing and shipping particulate samples to a laboratory. Reference N. B. Nelson, D. A. Siegel, C. A. Carlson, C. Swan, W. M. Smethie Jr. and S. Khatiwala. 2007. Hydrography of chromophoric dissolved organic matter in the North Atlantic. Deep-Sea Res. I. 54: 710 – 731. V. Kitidis, A. P. Stubbins, G. Uher, R. C. Upstill Goddard, C. S. Law, E. M. S. Woodward, “Variability of chromophoric organic matter in surface waters of the Atlantic Ocean”, Deep Sea Research Part II: Topical Studies, Vol. 53, Issue 14-16, 2006, p. 16661684. R. L. Miller, M. Belz, C. Del Castillo, R. Trzaska, UPVIS Ultrapath System, Visible Light UPUV Ultrapath System, Ultraviolet & Visible Light The UltraPath system includes: Multiple pathlength cell, Tidas E with TidasDAQ/SpectraView software, FO-6000 light source (UPVIS) or D2H light source (UPUV), two FO-600-SMA1M optical fibers, PeriStar Pro peristaltic pump, silicone tubing, sample injector and Waveguide Cleaning Kit. Specify line voltage 501609 KIT-UPVIS-STARTUP KIT-UPUV-STARTUP 89575 Waveguide Cleaning Kit FO-600-SMA1M, 501609, 72100, 800120, 15807 FO-600-SMA1M, 501609, 72100, D2H-DB, D2H-HB, 15807 QFT1, Fiber Optic Holder for Glass Fiber Filters 187 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com