Microinjection System Zebrafish, C. Elegans, Drosophila, Xenopus Oocytes System shown here: 5052 Steel base plate, M10 Magnetic stand, M3301L Micromanipulator, 5430-ALL PicoNozzle kit with a µTip, FD35-100 Fluorodish, PZMIII Microscope with optional lighted base, E210 Micropipette storage jar, 504156 Regine tweezers #5, 14003 Vannas spring scissors, glass capillaries, 77020 glass tweezers, REG10 Regal 10µL pipetter, PV820 Pneumatic PicoPump and 801566 (801963) Miniature Vacuum Pump. PUMPS, MICROINJECTION ebrafish (Danio Rerio) are rapidly gaining in popularity as bio-medical research subjects because of the ability to generate high resolution, in vivo images of the embryos. Zebrafish are easy to maintain and produce a large number of offspring. Additionally, the embryos have a nearly transparent skin, making their development easily visible. These fish are used for a variety of disciplines, including neuroscience, genetics and aging studies. Serving scientist for over 45 years, WPI offers a variety of instruments for microinjection including pumps, pipetters, microscopes and more. One of our most popular pumps for microinjection is the PV820 Pneumatic PicoPump. Z The PV820 and PV830 Pneumatic PicoPumps were designed to simplify intracellular injection. You get repeatable microinjection in volumes ranging from picoliters to nanoliters. PV820 offers eject and hold pressure. The hold pressure prevents backfilling of the pipette by capillary action. In addition, the PV830 also has vacuum pressure which allows you to securely hold a cell with one pipette while you inject it with another. The volume injected is controlled by the inside diameter of the glass tip, the pressure and the time. Recently, WPI introduced its customizable Microinjection System with everything you need to get started. The basic system is shown here. Starting below, you will find many options and accessories to customize your system. Options for Customizing Your System INJECTOR ● PV820 Pneumatic PicoPump with Hold Pressure ● PV830 Pneumatic PicoPump with Hold Pressure and Vacuum ● UMPIII UltraMicroPump ● Nanoliter2010 Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use carefully regulated air pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. The versatile UMP3 injector uses microsyringes to deliver picoliter volumes. Microprocessorcontrolled Nanoliter 2010 uses direct piston displacement. 174 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com