Application Kits NanoFil PUMPS, MICROINJECTION The optional UMP3 stand in the photo below includes the small base (503084), openside clamp (14073-4) and 25cm rod (503070). Holder assembly for RPE-KIT. These kits are specially designed for eye research for injecting retinal pigment epithelium (RPE) and intraocular (IO) in addition to brain injection in mice. They need to be used with a NanoFil syringe and UMP3 to achieve accurate, repetitive, and oil free injection in the submicroliter range. Each kit includes two pieces of Silflex tubing (one for a spare), a holder assembly, spare gaskets, and an assortment of four tips — blunt for the RPE kit and beveled tips for the IO kit. Each kit comes with one each of 33, 34, 35 and 36 gauge tips so that first time users can find the best size for their application. The Silflex tubing is the most critical component of the kit. This 35 cm long, flexible tubing has a very precise outer diameter for airtight fitting with the syringe. It also has a small inner diameter for minimum dead volume, and is very durable when handled correctly. The SilFlex is coupled to the injection tip with a mechanism similar to that of the NanoFil. The dead volume of the entire kit (including the tubing) is less than 3 microliters. All of the components in the kit are constructed of inert, solvent resistant materials for easy cleaning after viral injection. RECOMMENDED ACCESSORIES RPE-KIT Retinal Pigment Epithelium (RPE) injection kit (SilFlex tubing, gasket, holder, and blunt tip mix) IO-KIT Intraocular (IO) injection kit (SilFlex tubing, holder, gasket, and beveled tipmix) 503207 Stand & Clamps 180 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com