HYDROGEN PEROXIDE & OXYGEN SENSOR GUIDE ISO-HPO-2 APPLICATION SENSOR DIAMETER RESPONSE TIME DETECTION LIMIT DRIFT SENSITIVITY PHYSIOLOGICAL INTERFERENCE Cell Cultures, etc. 2.0 mm <5 SEC (90%) <100 nM to 100 mM <0.1 pA/min 8 pA/µM none ISO-HPO-100 Tissue/ Microvessels 100 micron <5 SEC (90%) 1 nM to 1 mM <1.0 pA/min 1 pA/nM Contact WPI ISO-HPO-100H Hypodermic Sheath 100 micron <5 SEC (90%) <10 nM to 1 mM 1.0 pA/min 1 pA/nM Contact WPI ISO-HPO-100-L Tissue Bath 100 micron <5 SEC (90%) 1 nM to 1 mM <1.0 pA/min 1 pA/nM Contact WPI ISO-OXY-2 Cell Cultures, etc. 2.0 mm <10 SEC (90%) 0.1 % to 100% <1%/min N/A none Hydrogen Peroxide Sensors Hydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling. At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens. In the progress of human disease, uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes, such as xanthine oxidase, can occur (Prof. Victor Darley-Usmar, Univ. of Alabama, personal communication). Despite the recognized importance of this oxidant in biology real-time measurements at low concentration have been difficult. The hydrogen peroxide sensors developed by WPI are designed to compliment existing high sensitivity fluorescent approaches with direct quantitative measurement in biological samples in the low nM range. Four hydrogen peroxide sensors are currently available. The ISO-HPO-2 is a 2.0 mm stainless steel sensor, with replaceable membrane sleeves (#600012) and an internal refillable electrolyte (#100042). The sensor is designed for use in cell cultures and similar applications. The ISO-HPO-100 is a 100 micron tip diameter hydrogen peroxide micro sensor designed for use in tissues and similar applications. The sensor design is based on a flexible “activated” carbon fiber sensing electrode coated with a proprietary membrane that enhances hydrogen peroxide detection. Both sensors incorporate WPI’s proprietary combination electrode technology whereby the hydrogen peroxide sensing element and separate ISO-HPO-100 reference electrode are encased within a single Faraday-shielded probe design. This design has been shown to enhance performance during measurements and minimizes overall sensor size. BIOSENSING ISO-HPO-100H 600011 ISO-HPO-2 ISO-HPO-100 ISO-HPO-100-L ISO-HPO-100H 600012 100042 91580 ISO-HPO Startup Kit (recommended with first purchase) 2mm Shielded HPO Sensor & Cable 100 µm HPO Sensor*, pkg of 3 100 µm HPO Sensor, L-shaped*, pkg of 3 100 µm HPO Sensor in hypodermic sheath*, pkg of 3 Replacement Sleeve Kit for ISO-HPO-2, pkg of 4 ISO-HPO-2 Electrolyte (10 mL) Microsensor Adapter Cable ISO-HPO-2 * Requires 91580 Microsensor Adapter Cable Oxygen Sensors This sensor incorporates WPI’s proprietary combination electrode technology whereby the oxygen-sensing element and separate reference electrode are encased within a single shielded sensor design. A gas-permeable polymer membrane is fitted over the end of the sleeve, which allows oxygen to pass while blocking liquids, ions and particulate matter. Oxygen diffuses through the membrane. The voltage applied to the sensor is held at -0.7V when the monitoring device is on and the sensor is properly connected. The magnitude of the generated electrical current is determined by the rate of diffusion through the membrane. The rate is proportional to the partial pressure of oxygen outside the membrane. The current serves as a measure of the partial pressure of O2. The ISO-OXY-2 is a 2.0 mm stainless steel sensor, with replaceable membrane sleeves (#5378) and an internal refillable electrolyte (#7326). The sensor is similar in design to WPI’s popular OXELP oxygen sensor (see page 68). ISO-OXY-2 5377 5378 7326 2 mm Shielded Oxygen Sensor & Cable ISO-OXY Startup Kit (recommended with first purchase) Replacement Electrode Sleeve Kit, pkg of 4 ISO2 Filling Solution (electrolyte) Temperature Sensor Current (nA) 250 200 150 100 50 0 0 20 40 60 80 100 120 y = 2.2862x + 4.3844 R2 = 0.9999 Current (nA) Oxygen (%) N2 (0%) air (21%) O2 (100%) The temperature sensor (#ISO-TEMP-2) is based on a 2.0 mm tip diameter high quality miniature platinum RTD (Resistance Temperature Detector) electrode. This design has been shown to provide greater accuracy, stability and interchangeability during temperature measurements than traditional thermistor and thermocouple sensors. The ISO-TEMP-2 is included with the purchase of a system. ISO-TEMP-2 91580 2 mm Platinum RTD Temperature Sensor (requires #91580) Microsensor Adapter Cable Time (s) 54 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com