ISO-NOPF – unique flexible NO Current (pA) sensor! Designed for arteries, microvessels, in vivo applications, and similar applications. ISO-NOPF electrodes are the newest addition to WPI’s nitric oxide sensor family and are available in 100 µm and 200 µm diameters. Utlilzing the latest advances in nanotechnology and material science, scientists at WPI’s Sensor Laboratory have created these completely flexible and virtually unbreakable NO sensors. The new sensors are based on a composite graphite NO-sensing element combined with a reference electrode. The surface of the sensor is then coated with a unique multi-layered NO-selective membrane. 50 pA Sensor Body 60mm x < 2mm∅ 10 sec 4 nM 2 nM 8 nM Ag/AgCl flexible 100, 200 or 500µm 1 nM 5mm 2mm Time (s) Response of ISO-NOPF to NO. Schematic drawing of ISO-NOPF. ISO-NOPF100 ISO-NOPF200 ISO-NOPF500 91580 SNAP50 100 µm Flexible NO Sensor, pkg of 2 200 µm Flexible NO Sensor, pkg of 2 500 µm Flexible NO Sensor, pkg of 2 Microsensor Adapter Cable SNAP, 50 mg vial of 30 microns and is available in two different tip lengths (i.e., ISONOP3020 has tip length of 2 mm, ISO-NO3005 has tip length of 0.5 mm). The response of the ISO-NOP007 and ISO-NOP30 is linear over a wide dynamic concentration range of NO. The design of both electrodes is based on a single carbon fiber coated with WPI’s NOselective membrane. A detection limit of approximately 1 nM NO makes these electrodes ideal for use in tissues and microvessels. performance — ideal for tissues and microvessels ISO-NOP007 ISO-NOP30 – 7 and 30 micron sensors with exceptional BIOSENSING The ISO-NOP007 and ISO-NOP30 have recently been improved in design and performance. The ISO-NOP007 has a tip diameter of just 7 microns and a length of 2 mm. The ISO-NOP30 has a tip diameter Current (pA) 300 pA 20 sec 1600 Sensor Body Ag/AgCl 60 mm x < 2 mm diam. 2 mm or 0.5 mm Current / pA 1200 y = 1.3583x + 16.15 R2 = 0.9985 800 7 µm or 30 µm 400 0 0 300 600 900 1200 Concentration / nM Time (s) Schematic drawing of ISO-NOP007 and ISONOP30. 2 mm The response of a 7 µm NO sensor (ISO-NOP007) to successive additions of NO (100 nM). Inset shows the linearity of the resulting calibration plot. ISO-NOP007 ISO-NOP3020 ISO-NOP3005 91580 SNAP50 7 µm Nitric Oxide Sensor (pkg of 3) 30 µm Sensor Tips (2 mm length), pkg of 3 (requires #91580) 30 µm Sensor Tips (0.5 mm fiber), pkg of 3 (requires #91580) Microsensor Adapter Cable SNAP, 50 mg vial Simultaneous measurement of force (top trace) and changes of NO concentration (lower trace) in (A) the rat superior mesenteric artery relaxed with ACh and (B) a small human artery relaxed with ACh and SNAP. In this artery oxyhaemoglobin(oxyHb) partly reversed the increase in NO concentration, with only a small change in force. [U. Simonsen, et al., J. Physiol., 1999, 516: 271-282.] FORT-100 Transducer ISO-NOP30L ISO-NOP70L ISO-HPO-100-L ISO-NOPF200-L10 L-shaped sensors for tissue bath & cell culture studies The ISO-NOP30-L is a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications (e.g., see WPI’s MYOBATH). The shape of the sensor has been engineered to facilitate ISO-NOP30-L ISO-NOP-70-L ISO-NOPF200-L10 ISO-HPO-100-L 91580 placement of the electrode within the lumen of the tissue vessel under study. The ISO-NOP70-L is similar in construction to the ISO-NOP30 but with the advantage of having a flexible tip (70 µm diameter). The ISO-NOPF200-L10 is designed specifically for cell culture studies. Micrometer Connect to Microsensor Cable #15810 Held in micromanipulator 3 mm carbon fiber 10 mm NO Sensor, L-Shaped 30-micron (pkg of 3) NO Sensor, L-Shaped 70-micron (pkg of 2) NO Sensor, 200 µm Flexible L-shaped (pkg of 2) HPO Sensor, L-Shaped 100-micron (pkg of 2) Microsensor Adapter Cable World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com 53