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67
W
orld Pr
ec
ision Instruments
"D"
"C"
"E"
"A
"
"B"
"F"
A
DETAIL A
Tip Order
Number
Tip O.D.
"A"
Tip I.D.
"B"
Tip
Length
"C"
Total
Length
"D"
Shank
O.D.
"E"
Bevel
Length
"F"
Total
Dead
Volume
Tip
Material
NF33BV-2
210 m
115 m
10.0 mm
40.0 mm
460 m
348 m 1.47 nL
Stainless Steel
NF34BV-2
185 m
85 m
5.0 mm
35.0 mm
460 m
290 m 1.39 nL
Stainless Steel
NF35BV-2
135 m
55 m
5.0 mm
35.0 mm
460 m
204 m 1.38 nL
Stainless Steel
NF36BV-2
110 m
35 m
3.0 mm
33.0 mm
460 m
156 m 1.37 nL
Stainless Steel
NF33FBV-2
200 m
100 m
5.0 mm
35.0 mm
460 m
322 m 1.40 nL
Titanium Alloy
NFQ34-5
160 m
100 m
55.0 mm
75.0 mm
460 m
280 m 0.59 nL
Quartz
NF33BL-2
210 m
115 m
10.0 mm
34.0 mm
460 m
348 m 1.47 nL
Stainless Steel
NF34BL-2
185 m
85 m
5.0 mm
29.0 mm
460 m
290 m 1.39 nL
Stainless Steel
NF35BL-2
135 m
55 m
5.0 mm
29.0 mm
460 m
204 m 1.38 nL
Stainless Steel
NF36BL-2
110 m
35 m
3.0 mm
27.0 mm
460 m
156 m 1.37 nL
Stainless Steel
NF33FBl-2
200 m
100 m
5.0 mm
29.0 mm
460 m
322 m 1.40 nL
Titanium Alloy
Above dimensions apply to blunt tips also.
penetrates easier and lasts longer than other
manufacturer's 33 gauge, 10 degree single
beveled tips. With a 35 gauge tri-surface
beveled tip, the resistance to the penetration
becomes even less. Each of our tips undergo a
penetration test before leaving the factory to
guarantee the best results for our customers.
Available Tips
33 gauge: This tip is similar to Hamilton's
7762 and 7803 series removable needles in
both tip length and outer diameter. However,
our beveled tip version is shorter, more durable,
and penetrates better due to the special tri-
surface grinding technique. In the past, 33
gauge tips were the smallest size sold by other
manufacturers and were frequently cited in
literature. However, our new 35 gauge tip
is much better for injections involving small
animals, especially mice. Compared with
Hamilton' 33 gauge, 10 degree beveled tip, our
35 gauge 25 degree beveled tip can reduce
the depth of penetration by almost 80%. The
distance between the tip and the upper rim
of the opening (section F on Figure 2) is 348
microns for the 33 gauge tip. The distance
for our 35 gauge tip is only 230 microns.
In addition, the smaller tip size significantly
reduces the required penetration force. In nearly
all applications, a 33 gauge tip can be replaced
with our 35 gauge tip and produce better
results.
34 gauge: This is a transitional size between
the 33 gauge and 35 gauge. If the 35 gauge
is too weak and the 33 gauge is too large, this
makes a good alternative.
35 gauge: This was the most popular and
preferred tip of most scientists during our field
trial. The combination of its strength, length,
durability, and clogging resistance creates a
balance with very little compromising of the
individual properties. It is much smaller than the
33 gauge tip offered by other manufacturers. It
is only slightly larger than the 36 gauge tip but
is much stronger and less likely to be clogged.
Samples can be directly loaded with this tip. Its
5 mm length is sufficient enough for almost all
injection applications in mice.
36 gauge: This is the smallest tip that is
commercially available. The tip is so small that
it can be inserted into the opening of the 33
gauge needle tip. Because this is pushing the
limits of what current technology can produce,
there are some limitations to consider before
using. Its thin diameter makes it necessary
to limit its length to 2.5 to 3.0 mm and still
maintain a usable strength. Since the tip ID is
in the 25 to 50 micron range, it is very easily
clogged. Therefore, only well filtered solutions
can be used. Depending on the viscosity of the
sample, the user might also need to pre-load
the syringe with a regular tip before switching to
this tip for injection. We recommend using the
35 gauge tip instead of the 36 gauge unless it is
absolutely necessary.
Flexifil: The Flexifil tip is made of a titanium
alloy. The advantage of this tubing is its
durability. This "semi-flexible" tip can be bent up
to 90 degrees without damage. It is also much
more corrosion resistant than the stainless steel
tip. Saline solutions left in the tip will be less
likely to clog it. Although this tip is specified
as a 33 gauge tip, its outer diameter is slightly
smaller than our 33 gauge stainless steel tip.
Flexible Quartz Tubing : The flexible quartz
tubing tip is made of 160 micron OD polyimide
coated quartz tubing with a special adapter
sleeve mounted at the end. It is designed for
filling glass capillary electrodes or pipettes, just
like WPI's traditional MF34G Microfil. However,
unlike the traditional MicroFil, which has about
50 microliters of dead volume in its luer hub,
the dead volume of this tip is less than 0.6
microliters. It is useful for loading electrodes
with solutions that have a limited volume or are
too expensive to waste.
World Precision Instruments
www.wpiinc.com
UK: Tel: +44 (0)1462 424700 · wpiuk@wpi-europe.com Germany: Tel: +49 (0)30-6188845 · wpide@wpi-europe.com US: Tel: 941-371-1003 · sales@wpiinc.com
World Precision Instruments
www.wpiinc.com
UK: Tel: +44 (0)1462 424700 · wpiuk@wpi-europe.com Germany: Tel: +49 (0)30-6188845 · wpide@wpi-europe.com US: Tel: 941-371-1003 · sales@wpiinc.com
NANOFIL NEEDLES
NF33BL-2
33 G blunt NanoFil needle (pkg of 2)
NF34BL-2
34 G blunt NanoFil needle (pkg of 2)
NF35BL-2
35 G blunt NanoFil needle (pkg of 2)
NF36BL-2
36 G blunt NanoFil needle (pkg of 2)
NF33BV-2
33 G beveled NanoFil needle (pkg of 2)
NF34BV-2
34 G beveled NanoFil needle (pkg of 2)
NF35BV-2
35 G beveled NanoFil needle (pkg of 2)
NF36BV-2
36 G beveled NanoFil needle (pkg of 2)
NF33FBL-2
33 G Flexifil blunt NanoFil needle (pkg of 2)
NF33FBV-2
33 G Flexifil beveled NanoFil needle (pkg of 2)
NF33-36BL
Assortment of 4 blunt NanoFil needles
NF33-36BV
Assortment of 4 beveled NanoFil needles
REPLACEMENT PARTS & ACCESSORIES
NFINHLD
NanoFil Injection Holder
SILFLEX-2
SilFlex tubing 35 cm long (pkg of 2) (dead volume = 2.74 L)
NFGSK-5
Spare Silicone Gasket for NanoFil & Holder (pkg of 5)
NFQ34-5
34 Gauge Flexible Quartz Tubing for filling (pkg 5)