DAM50

Overview

Benefits

• Very low internal noise
• Ultra quiet DC power supply ­— no AC required
• Intrinsic low susceptibility to ground loops
• Small footprint
• Cost-effective
• Electrostatic Discharge?Protection!

Applications

• Amplifying biopotentials from metal electrodes
• Brain slice field stimulation
• EAG (Electroantennogram)
• ERG (Electroretinogram)

Features

WPI’s DAM series amplifier’s are well known as a standard of the industry for extracellular potential amplification. These battery powered bio-amplifiers are designed with a compact chassis profile that enables the user to locate the unit closer to the preparation and thereby minimize long lead lengths which contribute to noise. Each amplifier is equipped with selectable high and low filters, and a position control to offset galvanic potentials which may develop during recording. A choice of models offer additional features that are useful for certain applications.

Portability

DAM series amplifiers can be used as standalone units on any tabletop, or use optional clamp-mounting hardware to locate them conveniently within the work area. Alternatively, a pair of amplifiers can be mounted into a standard equipment rack with a rack mount kit (#3484). A variety of hook up accessories are available to configure your application.

Using a DAM50 for EEG Recordings in Rodents

A low-noise amplifier like the DAM50 is an excellent choice for EEG recording in rodents. WPI’s amplifiers were engineered for the bio-medical researcher. While 20-30μV of noise is common in bio-amplifiers, WPI’s DAM series amplifiers generate 0.4μV RMS (root mean squared) at 0.1-100Hz. (That’s equal to about 2μV peak to peak.) This setup shows one way such recordings could be made. The RC1 electrode works well for rats, and the EP1 is more suitable for mouse cranial application.

Rat Cranial Applications

The RC1 electrode comes with a 4' unterminated wire attached. That needs to be soldered to the green-white ground wire of the 5470 cable.

1. The red wire from the 5470 cable connects with the non-inverting input on the amplifier, and the black wire connects with the inverting wire on the amplifier.
2. Solder a Profile A tungsten electrodes to the red and black wires.
3. For best results, seal the solder joints with a heat shrink tubing.
4. Affix the Profile A electrodes in the M3301EH electrode holders as shown in the images below.
5. Position the electrode holders into the two positions on the MD4R micromanipulator.
6. Position the electrodes so that the tips of the electrodes are nearly touching. These electrodes can then be precisely positioned in a rodent's brain.

 

Mouse Cranial Applications

The EP1 is a smaller electrode and is better suited for small rodents than the RC1 electrode. This application uses the same setup as the first one shown. In addition to the EP1 electrode, you will also need a length of wire to connect the green-white ground wire from the WPI #5470 cable with the EP1 electrode.

Feature Comparision Chart

	DAM50	DAM80
Input Mode	AC/DC	AC
Input Configuration	Differential/Single Ended	Differential
Gain Range	100-10,000 (AC) 10-1,000 (DC)	100 - 10,000 (AC)
High/Low Filters	Yes	Yes
Offset Position Control	Yes	Yes
Current Generator	No	Yes
Remote Active Headstage	No	Yes
Output Connection	BNC	3.5 mm mini phone
Standard Input Connection	unterminated wire	Mini banana
Power Supply	(2) 9V alkaline batteries	(2) 9V alkaline batteries

Specifications

INPUT IMPEDANCE	1012 Ω, common mode and differential
INPUT LEAKAGE CURRENT	50 pA (typical)
MAX. DC DIFFERENTIAL SIGNAL	±2.5 V (DAM 50)
GAIN	AC: 100x, 1000x, 10000x, DC: 10x, 100x, 1000x (DAM50)
COMMON MODE REJECTION RATIO	100dB @ 50/60 Hz
INPUT CAPACITANCE	20 pF
AC MODE NOISE	0.4 μV RMS (2uV p-p) 0.1-100 Hz
AC MODE NOISE	2.6 μV RMS (10uV p-p) 1 Hz-10 kH
DC MODE NOISE (DAM50)	(DAM50) 7.5uV RMS (30uV p-p) 3-10 kHz
BANDWIDTH FILTER SETTINGS:AC Mode	Low frequency, 0.1, 1, 10, 300 Hz
BANDWIDTH FILTER SETTINGS: DC Mode(DAM50)	High frequency, 0.1, 1, 3, 10 kHz
OUTPUT CONNECTORS	BNC
OUTPUT VOLTAGE SWING	±8 V
OUTPUT IMPEDANCE	470 Ω
BATTERY TEST	Audible tone
CALIBRATOR SIGNAL	10 Hz square wave
POSITION	Approximately 250 mV
EXTERNAL COMMAND	Input Voltage ±10 V commands
AC or DC current waveform	±50μA max. amplitude @ 200 KΩ
BATTERIES	2 x 9 V alkaline (included)
DIMENSIONS:DAM50	20.3 x 10.2 x 4.4 cm
SHIPPING WEIGHT	1.6 kg

The following bandwidth charts for the DAM50 show the response of the amplifier when various filters and gains are used.

GAINS: This chart shows the standard 3dB frequency cutoff at  maximum filter band pass. 

FILTERS: Various low pass and high pass filters were applied at the AC x1000 gain setting to show the bandwidth of and actual DAM50 amplifier. 

Accessories

Citations

Kim, E. Y., & Virginia, W. (n.d.). Effect of Growth Hormone on Hippocampal Synaptic Function during Sleep Deprivation By.

full-text. (n.d.).

Dai, J., Brooks, D. I., & Sheinberg, D. L. (n.d.). Supplemental Information Optogenetic and Electrical Microstimulation Systematically Bias Visuospatial Choice in Primates.

Škorjanc, A., & Belušic, G. (n.d.). How We Teach: Classroom And Laboratory Research Projects Investigation of blood flow and the effect of vasoactive substances in cutaneous blood vessels of Xenopus laevis.

Liu, Y., Wang, Y., Zhu, G., Sun, J., Bi, X., & Baudry, M. (2016). A calpain-2 selective inhibitor enhances learning & memory by prolonging ERK activation. Neuropharmacology, 105, 471–477. http://doi.org/10.1016/j.neuropharm.2016.02.022

Ztaou, S., Maurice, N., Camon, J., Guiraudie-Capraz, G., Kerkerian-Le Goff, L., Beurrier, C., … Amalric, M. (2016). Involvement of Striatal Cholinergic Interneurons and M1 and M4 Muscarinic Receptors in Motor Symptoms of Parkinson’s Disease. Journal of Neuroscience, 36(35).

Kentish, S. S. J., Frisby, C. L., Kritas, S., Li, H., Hatzinikolas, G., O’Donnell, T. A., … Ahern, G. (2015). TRPV1 Channels and Gastric Vagal Afferent Signalling in Lean and High Fat Diet Induced Obese Mice. PloS One, 10(8), e0135892. http://doi.org/10.1371/journal.pone.0135892

Blauvelt, D. G., Sato, T. F., Wienisch, M., & Murthy, V. N. (2013). Distinct spatiotemporal activity in principal neurons of the mouse olfactory bulb in anesthetized and awake states. Frontiers in Neural Circuits, 7. http://doi.org/10.3389/fncir.2013.00046

Blaise, J. H. (2013). Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice. Journal of Visualized Experiments, (81), e50642. http://doi.org/10.3791/50642

Wang, H., Siddharthan, V., Kesler, K. K., Hall, J. O., Motter, N. E., Julander, J. G., & Morrey, J. D. (2013). Fatal neurological respiratory insufficiency is common among viral encephalitides. The Journal of Infectious Diseases, 208(4), 573–83. http://doi.org/10.1093/infdis/jit186

Zhang, Q.-X., Lu, R.-W., Curcio, C. A., Yao, X.-C., DC., H., SE., N., … PN, D. (2012). In Vivo Confocal Intrinsic Optical Signal Identification of Localized Retinal Dysfunction. Investigative Opthalmology & Visual Science, 53(13), 8139. http://doi.org/10.1167/iovs.12-10732

Badsha, F., Kain, P., Prabhakar, S., Sundaram, S., Padinjat, R., Rodrigues, V., & Hasan, G. (2012). Mutants in Drosophila TRPC channels reduce olfactory sensitivity to carbon dioxide. PloS One, 7(11), e49848. http://doi.org/10.1371/journal.pone.0049848

Konow, N., Azizi, E., & Roberts, T. J. (2012). Muscle power attenuation by tendon during energy dissipation. Proceedings. Biological Sciences / The Royal Society, 279(1731), 1108–13. http://doi.org/10.1098/rspb.2011.1435

Chen, S., Mohajerani, M. H., Xie, Y., & Murphy, T. H. (2012). Optogenetic analysis of neuronal excitability during global ischemia reveals selective deficits in sensory processing following reperfusion in mouse cortex. The Journal of Neuroscience?: The Official Journal of the Society for Neuroscience, 32(39), 13510–9. http://doi.org/10.1523/JNEUROSCI.1439-12.2012

Morrey, J. D., Siddharthan, V., Wang, H., Hall, J. O., Motter, N. E., Skinner, R. D., & Skirpstunas, R. T. (2010). Neurological suppression of diaphragm electromyographs in hamsters infected with West Nile virus. Journal of Neurovirology, 16(4), 318–329. http://doi.org/10.3109/13550284.2010.501847

Brundage, C. M., & Taylor, B. E. (2010). Neuroplasticity of the central hypercapnic ventilatory response: teratogen-induced impairment and subsequent recovery during development. Developmental Neurobiology, 70(10), 726–35. http://doi.org/10.1002/dneu.20806

Kim, E., Grover, L. M., Bertolotti, D., & Green, T. L. (2010). Growth hormone rescues hippocampal synaptic function after sleep deprivation. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 298(6), R1588-96. http://doi.org/10.1152/ajpregu.00580.2009

Kavlie, R. G., Kernan, M. J., & Eberl, D. F. (2010). Hearing in Drosophila requires TilB, a conserved protein associated with ciliary motility. Genetics, 185(1), 177–88. http://doi.org/10.1534/genetics.110.114009 

Lenti, L., Domoki, F., Gáspár, T., Snipes, J. A., Bari, F., & Busija, D. W. (2009). N-Methyl- d -Aspartate Induces Cortical Hyperemia through Cortical Spreading Depression-Dependent and -Independent Mechanisms in Rats. Microcirculation, 16(7), 629–639. http://doi.org/10.1080/10739680903131510

Su, C.-K., Ho, C.-M., Kuo, H.-H., Wen, Y.-C., & Chai, C.-Y. (2009). Sympathetic-correlated c-Fos expression in the neonatal rat spinal cord in vitro. Journal of Biomedical Science, 16(1), 44. http://doi.org/10.1186/1423-0127-16-44

Brundage, C. M., & Taylor, B. E. (2009). Timing and duration of developmental nicotine exposure contribute to attenuation of the tadpole hypercapnic neuroventilatory response. Developmental Neurobiology, 69(7), 451–61. http://doi.org/10.1002/dneu.20720

Orem, N. R., Xia, L., & Dolph, P. J. (2006). An essential role for endocytosis of rhodopsin through interaction of visual arrestin with the AP-2 adaptor. Journal of Cell Science, 119(Pt 15), 3141–8. http://doi.org/10.1242/jcs.03052

Markham, M. R., & Stoddard, P. K. (2005). Adrenocorticotropic hormone enhances the masculinity of an electric communication signal by modulating the waveform and timing of action potentials within individual cells. The Journal of Neuroscience?: The Official Journal of the Society for Neuroscience, 25(38), 8746–54. http://doi.org/10.1523/JNEUROSCI.2809-05.2005

Cartford, M. C. (2004). Cerebellar norepinephrine modulates learning of delay classical eyeblink conditioning: Evidence for post-synaptic signaling via PKA. Learning & Memory, 11(6), 732–737. http://doi.org/10.1101/lm.83104

Jonker, D. M., Vermeij, D. A. C., Edelbroek, P. M., Voskuyl, R. A., Piotrovsky, V. K., & Danhof, M. (2003). Pharmacodynamic Analysis of the Interaction between Tiagabine and Midazolam with an Allosteric Model That Incorporates Signal Transduction. Epilepsia, 44(3), 329–338. http://doi.org/10.1046/j.1528-1157.2003.37802.x

van den Pol, A. N., Ghosh, P. K., Liu, R., Li, Y., Aghajanian, G. K., & Gao, X.-B. (2002). Hypocretin (orexin) enhances neuron activity and cell synchrony in developing mouse GFP-expressing locus coeruleus. The Journal of Physiology, 541(1), 169–185. http://doi.org/10.1113/jphysiol.2002.017426

Ransom, C. B., Ransom, B. R., & Sontheimer, H. (2000). Activity-dependent extracellular K+ accumulation in rat optic nerve: the role of glial and axonal Na+ pumps. The Journal of Physiology, 522 Pt 3, 427–42. Retrieved from http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2269766&tool=pmcentrez&rendertype=abstract

Alloway, P. G., & Dolph, P. J. (1999). A role for the light-dependent phosphorylation of visual arrestin. Proceedings of the National Academy of Sciences, 96(11), 6072–6077. http://doi.org/10.1073/pnas.96.11.6072

Lipchik, G. L., Holroyd, K. A., France, C. R., Kvaal, S. A., Segal, D., Cordingley, G. E., … McCool, H. R. (1996). Central and peripheral mechanisms in chronic tension-type headache. Pain, 64(3), 467–75. Retrieved from http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2128054&tool=pmcentrez&rendertype=abstract

Knuckey, N. W., Palm, D., Primiano, M., Epstein, M. H., & Johanson, C. E. (1995). N-Acetylcysteine Enhances Hippocampal Neuronal Survival After Transient Forebrain Ischemia in Rats. Stroke, 26(2), 305–311. http://doi.org/10.1161/01.STR.26.2.305

RelatedItems