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Nanoliter2020 Injector

Nanoliter2020 Injector

Precise nanoliter-volume injections with intuitive SMARTouch™ controller

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Nanoliter2020 with Smartouch controller Nanoliter2020 head Nanoliter2020 kit Nanoliter2020 run light Nanoliter2020 run light Nanoliter2020 on stereotaxic frame

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Quick start guide
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Instruction Manual
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Precise nanoliter-volume injections with intuitive SMARTouch™ controller

NANOLITER2020 is the complete system. 300704 is the NANOLITER2020 Injector Head only, and the controller and the accessories are not included.

  • Perform nanoliter volume injections using glass micropipettes
  • Nanoliter Injector works on the principle of Positive Displacement. An internal micrometer step motor precisely moves the metal plunger which pushes the oil inside the micropipette, and the oil-layer pushes nanoliter volumes of aqueous sample out of the pipette tip.
  • Fine control of plunger displacement along with proper sealing among gasket, glass micropipette, and oil ensures precision and accuracy
  • NANOLITER2020 System includes the 300704 NANOLITER Injector, the MICRO2T SMARTouch™ Controller and many necessary accessories (See Details)


Make Your Microinjection with Nanoliter Precision

Simple-to-Use Touchscreen Controller

NANOLITER2020 is the updated version of WPI’s popular NANOLITER2010 Injector that is capable of performing precision injections in the nanoliter range using a variety of glass micropipette sizes. The new model connects directly to the WPI MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump, allowing the pumps to be controlled individually or synchronously. The new injector head has a LED indicator which shows proper communication between the pump and controller. NANOLITER2020 offers improved precision by in-depth plunger displacement validation. The system is easier to use and has a chic silver injector head.




  • Minimizes loss of costly or scarce samples because injections are performed with mineral oil, back-filled, glass micropipettes, and the sample is front filled.
  • Control up to two pumps simultaneously
  • Mounts easily on a micromanipulator or a stereotaxic frame
  • Precise control over injection volumes and rates (nL to mL range)
  • Simple to use touchscreen interface and graphical representation of volume status on MICRO2T controller
  • LED indicator offers a visual indication of connectivity
  • Perform injections with a foot switch(optional)
  • Injector is compatible with different OD glass micropipettes (in the range of 1.1-1.5 mm)
  • Costs 15% less than the previous model (NL2010MC2T)!



The NANOLITER2020 Injector is perfect for making nanoliter to microliter range injections into frogs (Xenopus oocytes), rats, mice, mosquitoes, shrimps, insects (e.g., Brown Planthopper), flies (e.g., Drosophila) and fish (Zebrafish) embryo using glass micropipettes.


Important Notes

  • MICRO2T SMARTouch™ controller is required to operate the 300704 Injector (The same MICRO2T controller can be used with UMP3 pumps.)

  • 300746 Spare Parts Kit required for use with the 300704 Injector

  • Glass micropipettes are not included with the 300704. Use WPI glass micropipettes or use glass capillaries (504949 or 504950) and a puller to make micropipettes.

  • Use a tweezer (501981) to scoop gaskets out of the NANOLITER2020 Injector Head

  • 300704 Injector Head comes with one set of gaskets installed in the injector to use with 1.1-1.15 mm OD fire-polished glass



Order code Description
NANOLITER2020 Complete system (Injector and Controller)
300704 NANOLITER2020 Injector Head only
MICRO2T SMARTouch Controller only, Two-Channel


Benefits of NANOLITER2020 Injector (300704)

  • Injections performed with mineral oil back-filled glass micropipettes, and the sample is front filled. This approach minimizes loss of any costly and scarce sample when a minute sample volume can be adequate. (See FAQ for details.)
  • Control up to two pumps simultaneously
  • Mounts easily on a stereotaxic frame or a micromanipulator
  • Precise control over injection volumes (in the nanoliter to microliter range) and injection rates
  • Simple to use touchscreen interface and graphical representation of volume status on MICRO2T controller
  • LED run indicator offers a visual indication of connectivity
  • Perform the injection with a foot switch (optional)
  • Injector accepts a range of glass micropipettes (OD 1.1-1.5)
  • NANOLITER2020 costs 15% less than the previous model (NL2010MC2T)!


Nanoliter to microliter injection into frogs (Xenopus oocytes), rats, mice, mosquitoes, shrimps, insects (e.g., Brown Planthopper), flies (e.g., Drosophila) and fish (Zebrafish) embryo using glass micropipettes

Please refer to the “References” tab for details.

NANOLITER2020 Improvements

  • Connects directly to our MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump
  • The injector head has a LED indicator which lights up bright red in RUN mode and dim red when connected to the controller. The LED indicator shows proper communication between the pump and controller.
  • 14 mm OD glass micropipette is recommended for use with front green gasket. Use different OD fire-polished glass micropipettes by switching the front gasket (Green gasket for 1.1-1.15 mm OD glass, Black for 1.3-1.35 mm, Red for 1.5 mm).
  • Improved precision by in-depth plunger displacement validation
  • System is easier to use or operate
  • Injector has a chic silver design


NANOLITER2020 System includes all the components listed below.

NOTE: The 300704 Injector may also be purchase separately. 300704 does not include the Controller or any of the required accessories listed in the table below.

Order code Description Quantity

NANOLITER2020 Injector Head

[One Set of Gasket (Front Green Gasket) to use with 1.1-1.15 mm Glass is installed]
Items listed below are already included with NANOLITER2020 System or can be purchased separately:
MICRO2T SMARTouch Controller 1
501981 Tweezers to scoop out gaskets 1
504949 Glass Capillary (ID = 0.530 mm, OD 1.14 mm) 1 (pack of 300)
1TIP10XV119 Glass Micropipettes (ID = 0.530 mm, OD 1.14 mm, Tip ID= 10 μm) 2
300746 Spare Parts Kit includes:  
14456 Allen Wrench 0.035 Hex Tool 1
MF34G MicroFil 34 ga 1
300703 O-Ring Kit for NANOLITER2020 5
3563 1 cc Syringe 1
300514 Replacement Piston for NANOLITER2020 2

Two sample glass micropipettes are included. Use WPI-made glass micropipettes or use a glass capillary (504949) (included with NANOLITER2020) and a puller to make your own micropipettes.


  • What is the advantage of using oil back-filled glass micropipettes?

The term “back filling” refers to the process of filling the pipette from the large, non-pulled end.  “Front filling” is the term which is used to describe filling a micropipette through the small, pulled front end of the pipette. The glass micropipettes are first backfilled completely with mineral oil and secured to the NANOLITER Injector Head. Then, some mineral oil is dispensed through the tip. This creates the space and generates the pressure to front-fill samples through the tip. Front filling sample prevents the spillage or loss of costly or scarce sample involved with back-filling samples. When the sample volume is low, first back-filling glass micropipettes with oil and then front-filling the sample may be the only option.


  • Can I use WPI’s old standard controller to run the 300704 Injector head?

WPI NANOLITER2020 Injector Head (300704) needs the MICRO2T controller (recommended). WPI’s MICRO4 controller may be used to control the pump. The 300704 Injector Head cannot be used with WPI’s old standard controller.


  • Can I use same MICRO2T controller that I use with UMP3 pump? Do I need an additional adapter to use the 300704 pump with my existing MICRO2T controller?

Yes, the same MICRO2T controller can be used to control the UMP3 pump and the Nanoliter2010. No additional adapter is needed. 300704 connects directly to MICRO2T controller.


  • Can I run the pump using a footswitch?

Yes, you can use the footswitch 13142. This is not included in the NANOLITER2020 system and is sold separately.


  • What do I do when glass micropipettes come off after attaching or I am unable to front-fill a sample?

Odorless and colorless mineral oil is used for backfilling glass micropipettes. While the glass micropipette is installed and with gradual spillage over multiple uses, the gaskets become too slippery to hold glass micropipette in place. Use Kimwipes to soak up oil spillage inside the injector head, outside the glass micropipettes and to clean the gaskets. If needed, install a new set of gaskets to resolve the issue.

  • I position the  O-rings  in the right order and orientation per your video but still don’t get an air tight seal .What can be done?  

If the rear seal is loose or mounted backwards then it can leak from air passage. This gasket needs to be tight on the plunger wire. If the pipette is pull tested, then it can be easily unseated from the middle gasket and leak by air infusion. The butt end of the glass needs to be smooth (and without any cracks in the glass) and firmly held down onto the middle seat while the collet is being screwed down. That plastic seat will deform a small amount and "mold" itself to the end of the glass. The fluids can also flow out on their own without any added back pressure even if there is a good seal, but that amount of mixing at a 10 um tip is very small. If either rear gasket allows for air flow, then it will leak.


Using WPI's New NANOLITER2020 Injector


Setting Up Your Nanoliter 2020






Type Description
NANOLITER 2020 Plunger Outer Diameter 482 µm
Plunger Movement for 100 nL Volume Dispense 550 µm ± 55 µm
Piston Movement per dispensed volume (nL) 5.5 µm/nL
Minimum Volume (Injection) 5 nL
Recommended Glass
  • TIP10XV119 (Micropipette)
  • 504949 (Glass capillary)
  • 1.14 mm OD Fire Polished Glass
Glass Use Capabilities
  • 1.10-1.15 mm OD Glass with Green Front gasket
  • 1.30-1.35 mm OD Fire-polished Glass with Black Front Gasket
  • 1.5 mm OD Fire-polished Glass with Red Front Gasket
Minimum Injection Volume
  • 6nL
Maximum Possible Volume 4500 nL
Maximum Rate 644 nL/sec




O-ring Kit for Nanoliter2020

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Spare Parts Kit For Nanoliter2020

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WPI's previous versions of NANOLITER2020 (NANOLITER2010, NL2010MC2T, and NANOLITER2000) that worked on same oil-plunger based, positive-displacement technique were used for research studies involving injection into rats, mice, mosquitoes, shrimps, frogs (Xenopus oocytes), insects (e.g., Brown Planthopper), flies (e.g., Drosophila), and fish (Zebrafish) embryo.

Recent Publications

Gaete, P., Lillo, M., López, W., Liu, Y., Harris, A., & Contreras, J. (2020). A novel voltage clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels. BioRxiv, 2020.02.15.950923.

Atif, M., Lynch, J. W., & Keramidas, A. (2020). The effects of insecticides on two splice variants of the glutamate-gated chloride channel receptor of the major malaria vector, Anopheles gambiae . British Journal of Pharmacology, 177(1), 175–187.

Hao, D.-L., Yang, S.-Y., Liu, S.-X., Zhou, J.-Y., Huang, Y.-N., Véry, A.-A., … Su, Y.-H. (2020). Functional Characterization of the Arabidopsis Ammonium Transporter AtAMT1;3 With the Emphasis on Structural Determinants of Substrate Binding and Permeation Properties. Frontiers in Plant Science, 11, 571.

Li, Y., Liu, Z., Guo, Q., & Luo, M. (2019). Long-term Fiber Photometry for Neuroscience Studies. Neuroscience Bulletin, 35(3), 425–433.

Li, R., Weng, J., Wang, X., Meng, Q., Wang, Y., & Sun, J. (2019). Bursicon homodimers induce innate immune by activating the expression of anti-microbial peptide genes in the shrimp Neocaridina heteropoda. Fish and Shellfish Immunology, 84, 906–911.

Li, R., Huang, Y., Zhang, Q., Zhou, H., Jin, P., & Ma, F. (2019). The miR-317 functions as a negative regulator of Toll immune response and influences Drosophila survival. Developmental and Comparative Immunology, 95, 19–27.

Li, X., Liu, F., Wu, C., Zhao, J., Cai, W., & Hua, H. (2019). Decapentaplegic function in wing vein development and wing morph transformation in brown planthopper, Nilaparvata lugens. Developmental Biology, 449(2), 143–150.

Scala, R., Maqoud, F., Angelelli, M., Latorre, R., Perrone, M., Scilimati, A., & Tricarico, D. (2019). Zoledronic Acid Modulation of TRPV1 Channel Currents in Osteoblast Cell Line and Native Rat and Mouse Bone Marrow-Derived Osteoblasts: Cell Proliferation and Mineralization Effect. Cancers, 11(2), 206.



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Glass Capillaries for Nanoliter 2010, 300/vial - 3.5" long (ID 0.530 mm ± 25 µm, OD 1.1...

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Foot Switch for the MICRO2T, MICRO-ePORE and EVOM3 (13142)

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University of Oxford

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