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Nitric Oxide Detection

WPI offers the most extensive range of nitric oxide (NO) sensors available. Developed over a decade of extensive research in the field of nitric oxide, the result is a superior range of NO sensors that enable routine detection of nitric oxide at ultra low concentrations. 

The ideal nitric oxide sensor should be insensitive to other reactive species likely to be present within the measurement environment. The conventional Nafion coated carbon fiber nitric oxide sensor exhibits a large response to such species. WPI’s unique nitric oxide sensor technology utilizes a novel surface membrane which amplifies the response to NO while eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines, and much more.

• Excellent selectivity to NO - NaNO₂ (10^-6 or better) prevents contamination with other isoforms
• Rapid response time - < 5 sec enhances detection capability of the highly reactive, short-lived NO molecules
• Highly sensitive
• For use with TBR4100 and TBR1025

Nitric oxide (NO) is an essential signaling molecule and is known to play a significant role in a multitude of physiological systems including the central nervous system (CNS), the cardiovascular system, the gastrointestinal tract, the immune system, and the renal system. However, being highly reactive, detection and quantification of NO is very difficult. It requires a sensor that is sensitive, selective to NO, and easy to calibrate.

WPI’s Free Radical Analyzer (4-channel TBR4100  and single-channel TBR1025) and the LabTrax Data Acquisition System with the options of using nano NO sensors, micro NO sensors, flexible micro sensors, and macro nitric oxide sensors enable amperometric (electrical based) detection of NO dissolved in liquids. 

Nitric Oxide Sensor Guide

Applications	In Vivo	Cell Cultures, NO2, NO3	Tissue Bath	Micro Vessels	Micro Vessels	Single Cell
Sensor	ISO-NOPF	ISO-NOP	ISO-NOP30L	ISO-NOP3005 ISO-NOP3020	ISO-NOP007	ISO-NOPNM
Sensor Diameter	100, 200 or 500µm	2mm	30µm	30µm	7µm	100nm
Response Time	< 5 sec	< 5 sec	< 3 sec	< 3 sec	< 3 sec	< 3 sec
Lowest Detection Limit	0.2 nM	1 nM	1 nM	1 nM	0.5 nM	0.5 nM
Temperature Sensitivity	some	yes	yes	yes	yes	some
Drift	none	none	none	none	none	none
Sensitivity	10 pA/nM	2 pA/nM	1.4 pA/nM	1.4 pA/nM	1 pA/nM	0.5 pA/nM
Physiological Interferent	none	none	none	none	none	none

 

ISO-NOPF Options

Order code	Tip Length	Tip Diameter	Shape	Package of
ISO-NOPF100	1-5 mm	100 µm	Straight	2
ISO-NOPF200	1-5 mm	200 µm	Straight	2
ISO-NOPF200-L10	10 mm	200 µm	L-shaped	3
ISO-NOPF500-CXX	5-10 mm	500 µm	Straight	2
ISO-NOPF100-LXX	1-10 mm	100 µm	L-Shape	2
ISO-NOPF200-LXX	1-10 mm	200 µm	L-Shape	2

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