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Home > Products > Biosensing > Free Radical Detection > TBR Biosensors > Nitric Oxide Detection > ISO-NOP

ISO-NOP

Nitric Oxide Sensor - 2mm

Overview
Specifications
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Overview

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ISO-NOP Instruction Manual
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The original nitric oxide probe

Ideal for cell cultures, cell suspensions and many other applications.The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve. The tip of the sleeve is covered with a NO-selective membrane. Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325).

NO_‾3 and NO_‾2 Detection

A simple change in experimental protocol will enable the ISO-NOP to be conveniently used for rapid accurate determination of nitrite (NO‾2) and nitrate (NO‾3) concentration in samples. Using this method a detection limit for NO_‾2 or NO_‾3 as low as 1nM is routinely possible.

Selectivity of WPI's NO sensors

The ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment. Conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species. WPI's unique NO sensor technology utilizes an novel surface membrane which amplifies the response to NO whilst eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines, and much more.

Specifications

Outside Diameter	2mm
Response Time	< 5 sec
Lowest Detection Limit/Range	1nM
Upper Detection Limit/Range	80uM (beyond 80uM, sensor accuracy is not guaranteed)
Nominal Sensitivity (New sensor)	≤2pA/nM
Interferent (Selectivity Coefficient)	NaNO₂ (10^-6 or better)
Drift	< 1pA/min
Poise Voltage	865mV
Typical Quiescent Baseline Current, 25°C	3,000pA
Acceptable Baseline Range	1,000-8,000pA
Polarization Time	12+ hours
Recommended Polarization Solution	0.1M KI/H2SO4
Physiological Interference	None