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TEER Measurement in Lung In vitro Models

TEER Measurement in Lung In vitro Models


 

Epithelial and endothelial cells are known for their barrier function like selective permeability. Lung tissue is comprised of epithelial cellular layers, residing adjacent to endothelial layers and allowing exchange of oxygen and carbon dioxide between lung and blood. Currently, the biomedical research community’s major focus area is to understand the details of the infection caused by this novel coronavirus (2019-nCoV) or by similar viruses. This new virus can disrupt the lung’s normal cleaning ability to get rid of foreign particles in the lung, and the selective permeability or barrier function of the lung may become severely impaired. The adverse physiological effect may be sustained once the inflammatory cascade is initiated.

Researchers in collaboration with clinicians are trying to develop vaccines and drugs to prevent or treat the 2019-nCoV infection. Mammalian cell culture models are useful for basic understanding of the effect of viral infection on epithelial cells of the lung and endothelial cells of adjacent blood vessels or capillaries. Measurement of TEER can be useful in testing cytotoxicity of drugs and vaccines in lung epithelial, endothelial and epithelial-endothelial dual cell layer models. Testing the effectiveness of a therapeutic drug or agent in a cell culture model can also provide some meaningful information. Different lung cell culture models (including human) have been established to study epithelial transport in which TEER measurements provided useful information [Ren, et al. 2016, Dekali, et al. 2014 ].

In a number of previous studies, TEER measurement was one of the common methods that was used for studying the effect of influenza H1N1 virus infection on lung in vitro epithelial models [Wu, 2 et al. 2016, Short et al. 2016, Travanty, et al. 2015, Chen, et al. 2009]. In addition, researchers are coming up with in vitro 3-D lung tissue models to understand the details of lung infection at the cellular level and screen for drugs. [Reynar, et al. 2019, Chandorkar, et al. 2017, Sivars, et al. 2017, Braian, et al. 2015 (JOVE video ) Harrington, et al. 2014]. The simplicity of the experiments involving TEER measurement and the ability to correlate the TEER results with other transport experiments makes the evaluation of TEER a useful experimental tool to understand lung cellular physiology.

World Precision Instruments (WPI) is known to the global biomedical research community for their signature product line on TEER measurement. WPI offers the Epithelial Voltmeter Meter (EVOM2) along with the basic STX2/STX3 electrodes and also has options for advanced electrodes, such as EndOhm chambers and STX100 electrodes for higher precision and accuracy.

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Why should I choose TEER measurement by WPI’s EVOM2 system?

  • The EVOM2 device applies tiny microamp currents with continuous polarity switching (negative and positive) to prevent the development of electrical charge buildups on to the cellular layer. Therefore, minimal to negligible physiological effects are caused by the device or technique.
  • It is a non-destructive/non-invasive test. You can use the same sample for a different experiment.
  • TEER measurement is very quick (< 1 minute), and it is a simple test to perform.

 

What can I do with TEER measurement in lung epithelial-endothelial research?

  • Use TEER measurement to check the confluence of the monolayer of cells.
  • Evaluate the barrier function of the cellular monolayer (epithelial, endothelial or dual) to verify its integrity. Changes could indicate that the tight junction protein expression or localization may be affected or impaired (Short 2016 et al., Ren, et al. 2016, Wu, et al. 2016, Derk, et al. 2015, Rewaee, et al. 2013).
  • TEER is an indicator of permeability of cellular monolayers, and TEER measurement validates the results of permeability experiments with tracer molecules (such as, FITC-dextran, Lucifer yellow, etc.) [Wu, et al. 2016, Min, et al. 2016].
  • TEER values can be used as a preliminary and quick screening tool to test drug or virus cytotoxicity on in vitro lung models (Short, et al. 2016).
  • With WPI’s high throughput automated REMS system, sample resistance values of 96 samples can be measured in 10 minutes or less.

 

In addition to TEER measurement, the EVOM2 system provides the ability to measure trans-epithelial potential difference (TEPD).

 

What can I do with TEPD measurement in lung epithelial research?

  • Potential difference measurement is very simple to perform [Ren, et al. 2016 ]. No electrical input is involved in the millivolts mode. Therefore, the EVOM2 measurement technique does not affect the millivolt read outs. You can calculate the current flow [Zhao, et al. 2019] across cellular monolayers from Ohm’s Law I= V/R, where I= Current, V=Voltage and R=Resistance.
  • Evaluate the active transport pathways from TEPD. Select and use channel blockers or inhibitors, such as Epithelial Sodium Channel (ENaC) blocker (amiloride) [Zhao et al. 2019, Ren, et al. 2016] and Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) inhibitor (sulfinpyrazone) to investigate the contributions by specific channels and transporters in the overall active ion transport of the monolayers. Infection by Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) [Ji, et al. 2008] and influenza virus [Londino, et al. 2017, Chen, et al. 2004, Fang, et al. 2002] were reported to down-regulate ENaC activity. By comparing TEPD or calculated equivalent current of lung epithelial monolayers in the presence or absence of amiloride, useful ENaC activity data may be obtained in the context of viral infection.

 

WPI’s EVOM2 TEER measurement meter and REMS automated TEER measurement system are well documented research instruments that researchers rely on as our world continues the battle with viruses like the SARS coronavirus.

 

 

 


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EVA-MT-03-01

EVA-MT-03-01

EVOM Auto For TEER Measurement in 96 HTS Plate

System includes:

  • EVOM™ Auto (TEER Measurement System includes the Autosampler
  • HTS Electrode Array for 96 HTS Plates
  • Interface Unit and Cable
  • Control iPad with software


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EVA-MT-03-02

EVA-MT-03-02

EVOM Auto For TEER Measurement in Corning 24 HTS Plates

System includes:

  • EVOM™ Auto (TEER Measurement System includes the Autosampler
  • HTS Electrode Array for Corning 24 HTS Plates
  • Interface Unit and Cable
  • Control iPad with software


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EVA-MT-03-03

EVA-MT-03-03

EVOM Auto For TEER Measurement in Millipore 24 HTS Plates

System includes:

  • EVOM™ Auto (TEER Measurement System includes the Autosampler
  • HTS Electrode Array for Millipore 24 HTS Plates
  • Interface Unit and Cable
  • Control iPad with software


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REMS

REMS

DISCONTINUED - Automated Tissue Resistance Measuring System

REMS KIT

REMS KIT

REMS Kit

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EVM-MT-03-01

EVM-MT-03-01

EVOM Manual Epithelial Volt Ohm Meter for TEER Measurement

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REMS-24

REMS-24

Replacement REMS STX Electrode for 24-well HTS Plate. Use with REMS system.


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REMS-96

REMS-96

Replacement REMS STX Electrode for Millipore 96-well Plate. Use with REMS system.


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REMS-96C

REMS-96C

Replacement REMS STX Electrode for Corning 96-well Plate

ENDOHM-6G

ENDOHM-6G

EndOhm for 6mm Culture Cups (24 wells per plate)

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ENDOHM-12G

ENDOHM-12G

EndOhm for 12mm Culture Cups (12 wells per plate)

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ENDOHM-24G

ENDOHM-24G

EndOhm for 24mm and Costar Snapwell Culture Cups (6 wells per plate)

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EVM-EL-03-01-01

EVM-EL-03-01-01

ENDOHM-6 EVOM™ Electrode for TEER in 6.5 mm Insert, for use with EVOM Manual a...

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EVM-EL-03-01-02

EVM-EL-03-01-02

ENDOHM-12 EVOM™ Electrode for TEER in 12 mm Insert, for use with EVOM Manual a...

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EVM-EL-03-01-03

EVM-EL-03-01-03

ENDOHM-24 EVOM™ Electrode for TEER in 24 mm Insert, for use with EVOM Manual a...

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EVM-EL-03-02-05

EVM-EL-03-02-05

STX HTS EVOM™ Electrode for TEER in MatTek 96

  • Compatible with EVOM Manual and EVOM3 meters
  • For MatTek 96-well plates
  • Exclusively compatible with Evom Manual


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EVM-EL-03-03-02

EVM-EL-03-03-02

STX HTS EVOM™ Electrode for EVOM Manual, use with Millipore Multiscreen HTS 96-Well Plate

  • For Millipore Multiscreen HTS 96-Well Plate
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01. (Original Manufacture Replacement for ERS-2 part MERSSTX00)


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EVM-EL-03-03-03

EVM-EL-03-03-03

STX HTS EVOM™ Electrode for EVOM Manual, use with Corning Falcon HTS Multi Well Insert System

  • For Corning Falcon HTS Multi Well Insert System
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.


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EVM-EL-03-03-04

EVM-EL-03-03-04

STX HTS EVOM™ Electrode for EVOM Manual, use with Corning Costar HTS Transwell-24

  • For Corning Costar HTS Transwell-24
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.
     


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EVM-EL-03-03-05

EVM-EL-03-03-05

STX HTS EVOM™ Electrode for EVOM Manual, use with Corning HTS 96-Well Plate

  • For Corning HTS 96-Well Plate
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.

EVOM2

EVOM2

Trans Epithelial electric resistance (TEER) Measurements

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STX100C

STX100C

DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Corning Costar HTS Transwell-24

  • New model has a Mini XLR end for direct connection to EVOM Manual
  • Compatible with legacy meters including EVOM2 and Millipore ERS 2.0 when used in conjunction with Mini XLR to RJ-11 connector EVM-AC-02-01-01


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STX100C96

STX100C96

DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Corning HTS 96-Well Plate

  • New model has a Mini XLR end for direct connection to EVOM Manual
  • Compatible with legacy meters including EVOM2 and Millipore ERS 2.0 when used in conjunction with Mini XLR to RJ-11 connector EVM-AC-02-01-01


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STX100F

STX100F

DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Corning Falcon HTS Multi Well Insert System

  • New model has a Mini XLR end for direct connection to EVOM Manual
  • Compatible with legacy meters including EVOM2 and Millipore ERS 2.0 when used in conjunction with Mini XLR to RJ-11 connector EVM-AC-02-01-01


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STX100M

STX100M

DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Millipore Multiscreen HTS 96-Well Plate

  • New model has a Mini XLR end for direct connection to EVOM Manual
  • Compatible with legacy meters including EVOM2 and Millipore ERS 2.0 when used in conjunction with Mini XLR to RJ-11 connector EVM-AC-02-01-01


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EVOM3

EVOM3

DISCONTINUED - EVOM3 Trans Epithelial Electrical Resistance (TEER) meters.

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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