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Dr Gary Lawrence, Dublin City University

WPI are my preferred supplier for tissue and cell recording instrumentation. In my experience, WPI representatives have an excellent level of technical expertise and they have always responded in a timely manner with the most helpful, patient and instructive advice to aid the development of the o...
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NanoFil Syringe

NanoFil Syringe

NanoFil™ is a specially designed microliter syringe range developed for improved microinjection in mice and other small animals.The interchangable needles advance directly into the syringe barrel, creating a 1:1 connection with the plunger — a truly gas-tight system built for low-volume sample control. 


Features

  • NanoFil™ is the smallest dead volume microinjection system
  • Biologically compatible injection syringe
  • Various needle sizes available: 26-36G
  • Blunt or tri-beveled needles
  • Compatible with WPI's UMP3 microinjection system for precision injections

Benefits

  • Very low dead volume injections (0.5 µL or less)
  • Biologically compatible injection syringe (All the seals are constructed of inert materials for better chemical and biological compatibility)
  • Switching the syringe tip during an experiment is easy
  • Variety of injection tip options (needles and quartz tubing)
  • Unique tri-beveled needles for easier penetration

Applications

  • Animal research, viral targeting (e.g. AAV construct, DREADDS)
  • Capillary electrophoresis
  • Versatile research applications — Retinal Pigment Epithelial (RPE) and Intra Ocular (IO) injection kits

NanoFil Needles

The replaceable needles used with the NanoFil are available with either blunt or beveled tip designs. The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed. The beveled style NanoFil needles are used for applications that involve the penetration through a tough tissue. Nanofil needles are offered in sizes of 26G to 36G (OD of 460 µm to 120 µm).

Generally, a smaller needle tip diameter is better to limit diffusion occurring at the needle tip. A 36G needle would be the best suitable needle to restrict the diffusion at the tip of the needle. Any possible error resulting from diffusion when using a 36G NanoFil needle is expected to be negligible. (The error would be in the sub-nanoliter range). Thus, the use of NanoFil needles can help you maintain better accuracy during the injection of microliter and sub-microliter volumes.

 

Tip Order Number Guage Needle O.D.
“A” (µm)
Avg. Neelde I.D.
“B” (µm)
Tip Length
“C” (mm)
Total Length
“D” (mm)
Shank O.D.
“E” (µm)
Bevel Length
“F” (µm)
Total Dead Volume (µL)
NF33BV-2 33 210 115 10 40 460 ≈348 0.416
NF34BV-2 34 185 85 5 35 460 ≈290 0.199
NF35BV-2 35 135 55 5 35 460 ≈204 0.435
NF36BV-2 36 110 35 3 33 460 ≈156 0.340
NF33BL-2 33 210 115 10 40 460 ≈0 0.416
NF34BL-2 34 185 85 5 35 460 ≈0 0.199
NF35BL-2 35 135 55 5 35 460 ≈0 0.435
NF36BL-2 36 110 35 3 33 460 ≈0 0.340
NF26BV-2 26 460 110 3 40 460   0.380

Tip Material: Stainless Steel

Using NanoFil™ in different configurations

Direct injection by hand

This is the simplest and most economical way to inject. Any of our tips can be inserted directly into the NanoFil™ microliter syringe. Even the SilFlex tubing can be inserted to switch from hand injection to the other methods listed below. This method is limited by the accuracy of plunger movement that is achievable with a human hand.

Installed on WPI’s UMP3 microsyringe pump

This will allow the user to achieve nanoliter resolution and reproducibility. For neural system injection, mount the UMP3 on a stereotaxic frame.

SilFlex tubing and holder

The needle is mounted on a small plastic holder that is connected to the NanoFil by a 35 cm length of flexible tubing. The NanoFil syringe is mounted on the UMP3 pump. This configuration allows the user to hold the animal in one hand and insert the needle with the other. When the needle reaches the desired location, activate the pump using the footswitch and the pre-programmed injection volume will be delivered. This configuration gives a nanoliter level of accuracy and reproducibility. It is best suited for applications such as the RPE and IO injection.

 

Ordering Information

NanoFil Syringe

NANOFIL

NanoFil 10 µL Syringe
Includes (1) NF26BV 26 gauge needle, (1) 1 cc plastic syringe and (2) MF28G Microfil 28g needles for back filling the syringe

NANOFIL-100

NanoFil 100 µL Syringe
Includes (1) NF26BV 26 gauge needle

 

NanoFil Needles

NF33BL-2 33G Blunt NanoFil Needle 2/pk
NF34BL-2 34G Blunt NanoFil Needle 2/pk
NF35BL-2 35G Blunt NanoFil Needle 2/pk
NF36BL-2 36G Blunt NanoFil Needle 2/pk
NF33-36BL Blunt NanoFil Needle Assortment Pack 4/pk
NF33BV-2 33G Beveled NanoFil Needle 2/pk
NF34BV-2 34G Beveled NanoFil Needle 2/pk
NF35BV-2 35G Beveled NanoFil Needle 2/pk
NF36BV-2 36G Beveled NanoFil Needle 2/pk
NF33-36BV Beveled NanoFil Needle Assortment Pack 4/pk

 

Optional NanoFil Accessories & Replacement Parts

NF26BV-2 Replacement 26G Beveled NanoFil Needle 2/pk
NFINHLD NanoFil Injection Holder
NFINHLD-G10 1.0 mm Glass Pipette Holder for NanoFil Syringe
SILFLEX-2 SilFlex Tubing 35 cm Long 2/pk (Dead Volume = 2.74 µL)
NFQ34-5 34G Flexible Quartz Tubing for filling, 7.5 cm long 5/pk
IO-KIT Intraocular Injection Kit
RPE-KIT Retinal Pigment Epithelium Kit

 


NANOFIL

NANOFIL

NanoFil 10 µL Syringe

View details...

NANOFIL-100

NANOFIL-100

NanoFil 100 µL Syringe

View details...

IO-KIT

IO-KIT

Intraocular Injection Kit

View details...

RPE-KIT

RPE-KIT

Retinal Pigment Epithelium Kit

View details...


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NF33BL-2

NF33BL-2

33g Blunt NanoFil Needle 2/pk

  • Tip OD: 210 µm
  • Tip ID: 115 µm
  • Tip Length: 10.0 mm
  • Total Length: 40.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF34BL-2

NF34BL-2

34g Blunt NanoFil Needle 2/pk

  • Tip OD: 185 µm
  • Tip ID: 85 µm
  • Tip Length: 5.0 mm
  • Total Length: 35.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF35BL-2

NF35BL-2

35g Blunt NanoFil Needle 2/pk

  • Tip OD: 135 µm
  • Tip ID: 55 µm
  • Tip Length: 5.0 mm
  • Total Length: 35.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF36BL-2

NF36BL-2

36g Blunt NanoFil Needle 2/pk

  • Tip OD: 110 µm
  • Tip ID: 35 µm
  • Tip Length: 3.0 mm
  • Total Length: 33.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF26BV-2

NF26BV-2

26g Beveled Replacement NanoFil Needle 2/pk

  • Tip OD: 460 µm
  • Tip ID: 110 µm
  • Total Length: 40.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF33BV-2

NF33BV-2

33g Beveled NanoFil Needle 2/pk

  • Tip OD: 210 µm
  • Tip ID: 115 µm
  • Tip Length: 10.0 mm
  • Total Length: 40.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF34BV-2

NF34BV-2

34g Beveled NanoFil Needle 2/pk

  • Tip OD: 185 µm
  • Tip ID: 85 µm
  • Tip Length: 5.0 mm
  • Total Length: 35.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF35BV-2

NF35BV-2

35g Beveled NanoFil Needle 2/pk

  • Tip OD: 135 µm
  • Tip ID: 55 µm
  • Tip Length: 5.0 mm
  • Total Length: 35.0 mm
  • Tip Material: Stainless Steel
  • Package of 2


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NF36BV-2

NF36BV-2

36g Beveled NanoFil Needle 2/pk

  • Tip OD: 110 µm
  • Tip ID: 35 µm
  • Tip Length: 3.0 mm
  • Total Length: 33.0 mm
  • Tip Material: Stainless Steel
  • Package of 2

SILFLEX-2

SILFLEX-2

SilFlex flexible Tubing for Nanofil, 35cm long 2/pk

View details...


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NFINHLD

NFINHLD

NanoFil Injection Holder

  • For use with NANOFIL and SILFLEX for manual Injection

Use this holder for direct hand-held injections. The NFINHLD is 18 mm long and has a diameter of 9.6 mm (there are also two flat spots to 8.6 mm on the 0 and 180 degree axis). The cap is 8.1 mm diameter.


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NF33-36BL

NF33-36BL

Blunt NanoFil Needle Assortment Pack 4/pk

  • Includes one each of 33, 34, 35, and 36 g blunt needles
  • Tip Material: Stainless Steel
  • Package of 4


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NF33-36BV

NF33-36BV

Beveled NanoFil Needle Assortment Pack 4/pk

  • Includes one each of 33, 34, 35, and 36 g beveled needles
  • Tip Material: Stainless Steel
  • Package of 4


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NFGSK-5

NFGSK-5

Spare Silicone Gasket for NanoFil & Holder 5/pk

  • Replacement gaskets for NanoFil syringes and Holders
  • Package of 5
  • The colours may vary

NFINHLD-G10

NFINHLD-G10

1.0 mm Glass Pipette Holder for NANOFIL Syringe

View details...


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NFQ34-5

NFQ34-5

34g Flexible Quartz Tubing For Filling, 75.0 mm Long 5/pk

  • Tip OD: 160 µm
  • Tip ID: 100 µm
  • Tip Length: 55.0 mm
  • Total Length: 75.0 mm
  • Tip Material: Quartz
  • Package of 5

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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