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NanoFil Syringe

NanoFil™ is a specially designed microliter syringe range developed for improved microinjection in mice and other small animals.The interchangable needles advance directly into the syringe barrel, creating a 1:1 connection with the plunger — a truly gas-tight system built for low-volume sample control. 

Features

• NanoFil™ is the smallest dead volume microinjection system
• Biologically compatible injection syringe
• Various needle sizes available: 26-36G
• Blunt or tri-beveled needles
• Compatible with WPI's UMP3 microinjection system for precision injections

Benefits

• Very low dead volume injections (0.5 µL or less)
• Biologically compatible injection syringe (All the seals are constructed of inert materials for better chemical and biological compatibility)
• Switching the syringe tip during an experiment is easy
• Variety of injection tip options (needles and quartz tubing)
• Unique tri-beveled needles for easier penetration

Applications

• Animal research, viral targeting (e.g. AAV construct, DREADDS)
• Capillary electrophoresis
• Versatile research applications — Retinal Pigment Epithelial (RPE) and Intra Ocular (IO) injection kits

NanoFil Needles

The replaceable needles used with the NanoFil are available with either blunt or beveled tip designs. The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed. The beveled style NanoFil needles are used for applications that involve the penetration through a tough tissue. Nanofil needles are offered in sizes of 26G to 36G (OD of 460 µm to 120 µm).

Generally, a smaller needle tip diameter is better to limit diffusion occurring at the needle tip. A 36G needle would be the best suitable needle to restrict the diffusion at the tip of the needle. Any possible error resulting from diffusion when using a 36G NanoFil needle is expected to be negligible. (The error would be in the sub-nanoliter range). Thus, the use of NanoFil needles can help you maintain better accuracy during the injection of microliter and sub-microliter volumes.

 

Tip Order Number	Guage	Needle O.D. “A” (µm)	Avg. Neelde I.D. “B” (µm)	Tip Length “C” (mm)	Total Length “D” (mm)	Shank O.D. “E” (µm)	Bevel Length “F” (µm)	Total Dead Volume (µL)
NF33BV-2	33	210	115	10	40	460	≈348	0.416
NF34BV-2	34	185	85	5	35	460	≈290	0.199
NF35BV-2	35	135	55	5	35	460	≈204	0.435
NF36BV-2	36	110	35	3	33	460	≈156	0.340
NF33BL-2	33	210	115	10	40	460	≈0	0.416
NF34BL-2	34	185	85	5	35	460	≈0	0.199
NF35BL-2	35	135	55	5	35	460	≈0	0.435
NF36BL-2	36	110	35	3	33	460	≈0	0.340
NF26BV-2	26	460	110	3	40	460		0.380

Tip Material: Stainless Steel

Using NanoFil™ in different configurations

Direct injection by hand

This is the simplest and most economical way to inject. Any of our tips can be inserted directly into the NanoFil™ microliter syringe. Even the SilFlex tubing can be inserted to switch from hand injection to the other methods listed below. This method is limited by the accuracy of plunger movement that is achievable with a human hand.

Installed on WPI’s UMP3 microsyringe pump

This will allow the user to achieve nanoliter resolution and reproducibility. For neural system injection, mount the UMP3 on a stereotaxic frame.

SilFlex tubing and holder

The needle is mounted on a small plastic holder that is connected to the NanoFil by a 35 cm length of flexible tubing. The NanoFil syringe is mounted on the UMP3 pump. This configuration allows the user to hold the animal in one hand and insert the needle with the other. When the needle reaches the desired location, activate the pump using the footswitch and the pre-programmed injection volume will be delivered. This configuration gives a nanoliter level of accuracy and reproducibility. It is best suited for applications such as the RPE and IO injection.

 

Ordering Information

NanoFil Syringe

NANOFIL	NanoFil 10 µL Syringe Includes (1) NF26BV 26 gauge needle, (1) 1 cc plastic syringe and (2) MF28G Microfil 28g needles for back filling the syringe
NANOFIL-100	NanoFil 100 µL Syringe Includes (1) NF26BV 26 gauge needle

 

NanoFil Needles

NF33BL-2	33G Blunt NanoFil Needle 2/pk
NF34BL-2	34G Blunt NanoFil Needle 2/pk
NF35BL-2	35G Blunt NanoFil Needle 2/pk
NF36BL-2	36G Blunt NanoFil Needle 2/pk
NF33-36BL	Blunt NanoFil Needle Assortment Pack 4/pk
NF33BV-2	33G Beveled NanoFil Needle 2/pk
NF34BV-2	34G Beveled NanoFil Needle 2/pk
NF35BV-2	35G Beveled NanoFil Needle 2/pk
NF36BV-2	36G Beveled NanoFil Needle 2/pk
NF33-36BV	Beveled NanoFil Needle Assortment Pack 4/pk

 

Optional NanoFil Accessories & Replacement Parts

NF26BV-2	Replacement 26G Beveled NanoFil Needle 2/pk
NFINHLD	NanoFil Injection Holder
NFINHLD-G10	1.0 mm Glass Pipette Holder for NanoFil Syringe
SILFLEX-2	SilFlex Tubing 35 cm Long 2/pk (Dead Volume = 2.74 µL)
NFQ34-5	34G Flexible Quartz Tubing for filling, 7.5 cm long 5/pk
IO-KIT	Intraocular Injection Kit
RPE-KIT	Retinal Pigment Epithelium Kit

 

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