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Biochips For Cell Adhesion & Culture Under Flow

Biochips For Cell Adhesion & Culture Under Flow

WPI offers Cellix Biochips for a wide range of applications across the life sciences including biofilm studies, shear stress studies, ligand studies, rolling and adhesion assays, and single cell studies.


Biochips For Cell Adhesion & Cell Culture Under Flow

Vena8 Fluoro+ Biochip

Cell-Ligand / Cell-Protein adhesion

Each biochip contains 8 capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow.  

These biochips are compatible with confocal microscopy.

  • Under shear flow
  • Disposable biochip
  • Cell adhesion, rolling assays on ligand/protein coated channels
  • Ideal for platelet aggregation & thrombosis assays

Vena8 Fluoro+ Biochip Technical Notes

 

Vena8 Fluoro+ Biochip Protocol Notes

 

Product Code Product Description
V8CF-400-100-02P5 Vena8 Fluoro+ Biochip Pack 5
V8CF-400-100-02P10 Vena8 Fluoro+ Biochip Pack 10

 

Vena8 Endothelial+ Biochip

Cell culture & cell-cell adhesion

Each biochip contains 8 capillaries in parallel which can be seeded with endothelial cells for culture of 8 monolayers in parallel and subsequent study of cell-cell interaction studies under shear flow.

  • Under shear flow
  • Disposable biochip
  • Cell culture of endothelial cell monolayers in microchannel flow chambers
  • Cell-cell adhesion shear flow assays

Vena8 Endothelial+ Biochip Technical Notes

 

Vena8 Endothelial+ Biochip Protocol Notes

 

Product Code Product Description
V8EP-800-120-02P5 Vena8 Endothelial+ Biochip Pack 5
V8EP-800-120-02P10 Vena8 Endothelial+ Biochip Pack 10

 

 

Vena8 Biochip With Glass Coverslips

Cell-Ligand & cell-cell adhesion

  • Under shear flow
  • Disposable biochip
  • Flexibility with glass coverslip supplied unattached.
  • Glass coverslips may be treated with chelated Cu2+ ion tethered to the high density PEG-coating

Options:

  • Coverslip: 

  • Glass coverslip adhered

  • Glass coverslip not adhered

  • Adhesive layer adhered

  • Surface treatment of chip:

    • Not treated

    • Treated with Cu2+ histidine tag

    • Treated with NHS leaving group on PEG brush

  • Suitable for flow rates:
      • Low Flow rates (1600 µm x 160 µm channel size)

      • High Flow rates (800 µm x 80 µm channel size)

Vena8 Biochip With Glass Coverslips Technical Notes

 

Vena8 Biochip With Glass Coverslips Low Shear Flow Rates

 

Vena8 Biochip With Glass Coverslips High Shear Flow Rates

 

Product Code Product Description
V8GCS-A-NT-1600-160-P10 Vena8 with Glass Coverslips Adhered, Non-treated; Low Flow Rates; Pack 10
V8GCS-A-NT-800-80-P10 Vena8 with Glass Coverslips Adhered, Non-treated; High Flow Rates; Pack 10
V8GCS-NA-NT-1600-160-P10 Vena8 with Glass Coverslips Not Adhered, Non-treated; Low Flow Rates; Pack 10
V8GCS-NA-NT-800-80-P10 Vena8 with Glass Coverslips Not Adhered, Non-treated; High Flow Rates; Pack 10
V8-Adhesive-1600-160-P10 Vena8 with adhesive bottom layer; Low Flow Rates; Pack 10
V8-Adhesive-800-80-P10 Vena8 with adhesive bottom layer; High Flow Rates; Pack 10
V8GCS-A-T-Cu2+Htag-1600-160-P10 Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; Low Flow Rates; Pack 10
V8GCS-A-T-Cu2+Htag-800-80-P10 Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; High Flow Rates; Pack 10
V8GCS-A-T-NHS_02-1600-160-P10 Vena8 with Glass Coverslips Adhered, Treated with NHS leaving group on PEG brush; Low Flow Rates; Pack 10
V8GCS-A-T-NHS_02-800-80-P10 Vena8 with Glass Coverslips Adhered, Treated with NHS leaving group on PEG brush; High Flow Rates; Pack 10

 

Vena Delta Y1 Biochip

The VenaDeltaY1 biochips contain branching microchannels for dual flow / dual injection of samples.  Ideal for studying chemotactic gradients, dual flow, multilaminar flow & diffusion.

Each biochip contains 4 Y-shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.

  • Branching Y-junction at input port
  • Mimic branching microvascular networks
  • Chemotaxis assays, dual flow, multilaminar flow & diffusion
  • Choice of channel size 400 µm (width) x 100 µm (depth) or 800 µm (width) x 120 µm (depth)

Vena8 Delta Y BiochipTechnical Notes

 

Product Code Product Description
V4DY1-400-100-1-1P10 VenaDeltaY1 Biochip Pack 10 (Minimum order of 6 packs) - channel size 400 µm (width) x 100 µm (depth)
V4DY1-800-120-1-1P10 VenaDeltaY1 Biochip Pack 10 (Minimum order of 6 packs) - channel size 800 µm (width) x 120 µm (depth)

 

Vena Delta Y2 Biochip

The VenaDeltaY2 biochips contain branching microchannels for dual flow / dual injection of samples.  Ideal for studying chemotactic gradients, dual flow, multilaminar flow & diffusion.

Each biochip contains 4 Y-shaped capillaries at both ends in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.

  • Branching Y-junction at input & exit ports
  • Mimic branching microvascular networks
  • Chemotaxis assays, dual flow, multilaminar flow & diffusion
  • Channel sizes:  600 µm (width) x 100 µm (depth)

Vena8 Delta Y BiochipTechnical Notes

 

Product Code Product Description
V4DY2-600-100-2-1P10 VenaDeltaY2 Biochip Pack 10 (Minimum order of 6 packs)
 

Vena Delta T Biochip

The VenaDeltaT biochip contains 4 T-junction shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the T-channel branch.

  • 4 T-junction shaped capillaries
  • Channel sizes:  400 µm (width) x 100 µm (depth)
Product Code Product Description
V4DT-400-100-1-1P10 VenaDeltaT Biochip Pack 10
 

Vena Delta W Biochip

The VenDeltaW biochip contains 2 W-junction shaped capillaries and 1 Y-junction shaped capillary in the centre which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the W or Y-channel branch.

  • 2 W-junction shaped capillaries
  • 1 Y-junction shaped capillary
  • Channel sizes:  400 µm (width) x 100 µm (depth)
Product Code Product Description
VDW-400-100-1-1P10 VenaDeltaW Biochip Pack 10

 



Choose Quantity
V8CF-400-100-02P10

V8CF-400-100-02P10

Vena8 Fluoro+ Biochip Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow.  

Each 10 pack contains 80 assays.

These biochips are compatible with confocal microscopy.  

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.


Choose Quantity
V8CF-400-100-02P5

V8CF-400-100-02P5

Vena8 Fluoro+ Biochip Pack 5

Each biochip contains 8 capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow.  

Each 5 pack contains 40 assays.

These biochips are compatible with confocal microscopy.  

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.


Choose Quantity
V8EP-800-120-02P10

V8EP-800-120-02P10

Vena8 Endothelial+ Biochip Pack 10

Each biochip contains 8 capillaries in parallel which can be seeded with endothelial cells for culture of 8 monolayers in parallel and subsequent study of cell-cell interaction studies under shear flow.  

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.


Choose Quantity
V8EP-800-120-02P5

V8EP-800-120-02P5

Vena8 Endothelial+ Biochip Pack 5

Each biochip contains 8 capillaries in parallel which can be seeded with endothelial cells for culture of 8 monolayers in parallel and subsequent study of cell-cell interaction studies under shear flow.  

Each 5 pack contains 40 assays.

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.


Choose Quantity
VT4-400-100-2-1P10

VT4-400-100-2-1P10

VenaT4 Biochip Pack 10

Each biochip contains 4 capillaries in parallel with a membrane of pore size 2-10 um diameter separating them from a microwell underneath.  Chemoattractants can be placed inside the well for subsequent study of cell migration through the membrane under shear flow.  

Each pack contains 10 biochips = 40 assays.  

Pore size choices:  2um; 5um; 8um or 10um.


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V4DY1-400-100-1-1P10

V4DY1-400-100-1-1P10

VenaDeltaY1 Biochip Pack 10 - Dimensions of microchannels:  400 (W) x 100 (D)

Each biochip contains 4 Y-shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.  

Each pack contains 10 biochips = 40 assays.  

There are two different sizes of microchannels available.  Dimensions of microchannels:  400 (W) x 100 (D).  

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.

MINIMUM ORDER OF 6 PACKETS


Choose Quantity
V4DY1-800-120-1-1P10

V4DY1-800-120-1-1P10

VenaDeltaY1 Biochip Pack 10 - Dimensions of microchannels:  800 (W) x 120 (D)

Each biochip contains 4 Y-shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.  

Each pack contains 10 biochips = 40 assays.  

There are two different sizes of microchannels available.  Dimensions of microchannels:  800 (W) x 120 (D).

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.

MINIMUM ORDER OF 6 PACKETS


Choose Quantity
V4DY2-600-100-2-1P10

V4DY2-600-100-2-1P10

VenaDeltaY2 Biochip Pack 10 - Dimensions of microchannels:  600 (W) x 100 (D)

Each biochip contains 4 Y-shaped capillaries at both ends in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.

Each pack contains 10 biochips = 40 assays.

Dimensions of microchannels:  600 (W) x 100 (D).

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.

MINIMUM ORDER OF 6 PACKETS


Choose Quantity
V4DT-400-100-1-1P10

V4DT-400-100-1-1P10

VenaDeltaT Biochip Pack 10

Each biochip contains 4 T-junction shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the T-channel branch.

Each pack contains 10 biochips = 40 assays.

Dimensions of microchannels:  400 (W) x 100 (D).

Compatible with Mirus Evo Nanopump, Kima pump.  Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.


Choose Quantity
VDW-400-100-1-1P10

VDW-400-100-1-1P10

VenaDeltaW Biochip Pack 10

Each biochip contains 2 W-junction shaped capillaries with one Y-junction shaped capillary in the centre which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the W or Y-channel branch.

Each pack contains 10 biochips = 30 assays.

Dimensions of microchannels:  400 (W) x 100 (D).

Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.


Choose Quantity
MIXED-BIOCHIPS-02P10

MIXED-BIOCHIPS-02P10

Mixed Biochip Pack 10

Mixed biochip pack; may contain Vena8 Fluoro+; Vena8 Endothelial+ Biochips


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SAMPLE-BIOCHIPS-01P2

SAMPLE-BIOCHIPS-01P2

Sample Biochip Pack 2

Mixed biochip pack; may contain Vena8 Fluoro+; Vena8 Endothelial+ Biochips


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SAMPLE-VENAT4-BIOCHIPS-01P2

SAMPLE-VENAT4-BIOCHIPS-01P2

Sample VenaT4 Biochip Pack 2

2 x VenaT4 biochips.


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V8GCS-A-T-Cu2+Htag-1600-160-P10

V8GCS-A-T-Cu2+Htag-1600-160-P10

Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; Low Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The bottom substrate is a glass coverslip treated with chelated Cu2+ ion tethered to the high-density PEG coating.  The surface of the microcapillaries is easily coated via pipetting the protein into the capillary which then binds via the poly-histidine tag.  

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 0.1 - 2 dyne/ cm2 (water based solution) and 0.45 - 9 dyne/ cm2 (whole blood).  

Compatible with Kima pump for shear stresses of 1 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-A-T-Cu2+Htag-800-80-P10

V8GCS-A-T-Cu2+Htag-800-80-P10

Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; High Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The bottom substrate is a glass coverslip treated with chelated Cu2+ ion tethered to the high-density PEG coating.  The surface of the microcapillaries is easily coated via pipetting the protein into the capillary which then binds via the poly-histidine tag.

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 20 - 40 dyne/ cm2 (water based solution) and 90 -  180 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 4 - 10 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-A-T-NHS_02-1600-160-P10

V8GCS-A-T-NHS_02-1600-160-P10

Vena8 with Glass Coverslips Adhered, Treated with NHS leaving group on PEG brush; Low Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The bottom substrate is a glass coverslip treated with NHS leaving group on PEG brush for the immobilization of un-tagged protein, peptide or other -NH2 functional molecules.

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 0.1 - 2 dyne/ cm2 (water based solution) and 0.45 - 9 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 1 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-A-T-NHS_02-800-80-P10

V8GCS-A-T-NHS_02-800-80-P10

Vena8 with Glass Coverslips Adhered, Treated with NHS leaving group on PEG brush; High Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The bottom substrate is a glass coverslip treated with NHS leaving group on PEG brush for the immobilization of un-tagged protein, peptide or other -NH2 functional molecules.  

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 20 - 40 dyne/ cm2 (water based solution) and 90 -  180 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 4 - 10 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-A-NT-1600-160-P10

V8GCS-A-NT-1600-160-P10

Vena8 with Glass Coverslips Adhered, Non-treated; Low Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The bottom substrate is a glass coverslip which is non-treated .  

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 0.1 - 2 dyne/ cm2 (water based solution) and 0.45 - 9 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 1 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-A-NT-800-80-P10

V8GCS-A-NT-800-80-P10

Vena8 with Glass Coverslips Adhered, Non-treated; High Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The bottom substrate is a glass coverslip which is non-treated .

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 20 - 40 dyne/ cm2 (water based solution) and 90 -  180 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 4 - 10 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-NA-NT-1600-160-P10

V8GCS-NA-NT-1600-160-P10

Vena8 with Glass Coverslips Not Adhered, Non-treated; Low Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The protective cover on the bottom of the chips is peeled away to reveal adhesive tape to which glass coverslip which is non-treated (supplied) can be adhered.  It is also possible to adhere different materials that the user may have.

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 0.1 - 2 dyne/ cm2 (water based solution) and 0.45 - 9 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 1 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8GCS-NA-NT-800-80-P10

V8GCS-NA-NT-800-80-P10

Vena8 with Glass Coverslips Not Adhered, Non-treated; High Flow Rates; Pack 10

Each biochip contains 8 capillaries in parallel which can be coated with a protein or seeded with cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.

The protective cover on the bottom of the chips is peeled away to reveal adhesive tape to which glass coverslip which is non-treated (supplied) can be adhered.  It is also possible to adhere different materials that the user may have.

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 20 - 40 dyne/ cm2 (water based solution) and 90 -  180 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 4 - 10 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.


Choose Quantity
V8-Adhesive-1600-160-P10

V8-Adhesive-1600-160-P10

Vena8 with adhesive bottom layer; Low Flow Rates; Pack 10

These biochips have a protective cover which can be peeled away to reveal adhesive bottom layer to which any material may be attached by the user.

Each biochip contains 8 capillaries in parallel which can be assembled with a substrate from the user.

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 0.1 - 2 dyne/ cm2 (water based solution) and 0.45 - 9 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 1 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.

Particularly suited for biofilm applications with Kima pump achieving shear stresses of 1dyne/ cm2 where different substrate materials may be easily tested.  


Choose Quantity
V8-Adhesive-800-80-P10

V8-Adhesive-800-80-P10

Vena8 with adhesive bottom layer; High Flow Rates; Pack 10

These biochips have a protective cover which can be peeled away to reveal adhesive bottom layer to which any material may be attached by the user.

Each biochip contains 8 capillaries in parallel which can be assembled with a substrate from the user.  

Each 10 pack contains 80 assays.

Compatible with ExiGo, UniGo, 4U, Mirus for shear stresses of 20 - 40 dyne/ cm2 (water based solution) and 90 -  180 dyne/ cm2 (whole blood).

Compatible with Kima pump for shear stresses of 4 - 10 dyne/ cm2 (water based solution); Kima pump not compatible with whole blood.

Particularly suited for biofilm applications with Kima pump achieving shear stresses of 1dyne/ cm2 where different substrate materials may be easily tested.  

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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