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STX HTS EVOM™ Electrode for TEER

STX HTS EVOM™ Electrode for TEER

For high throughput screening (HTS) in cell culture filter plates


STX HTS EVOM & STX100 Instruction Manual

For high throughput screening (HTS) in cell culture filter plates

  • Designed for use with 24-well HTS plate (Corning Costar and Corning Falcon) and with 96-well plates (Corning, Millipore and MatTek)
  • Improved accuracy down to 5 Ω
  • Sterilized with EtO, alcohol or bactericide

Options

The STX HTS EVOM electrode are available with a Mini XLR connector compatible for the EVOM Manual and EVOM3 meter. To connect with the EVOM, EVOM2™, and Millicell® ERS and ERS2 use the Mini XLR to RJ-11 connector EVM-AC-02-01-01. Use the tables below to select the required option.

STX HTS compatible with EVOM™ Manual and EVOM3

Order code Description
EVM-EL-03-03-05 STX HTS EVOM™ Electrode for TEER in Corning 96
EVM-EL-03-03-02 STX HTS EVOM™ Electrode for TEER in Millipore 96
EVM-EL-03-03-03 STX HTS EVOM™ Electrode for TEER in Corning Biocoat/Falcon 24
EVM-EL-03-03-04 STX HTS EVOM™ Electrode for TEER in Corning Costar 24
EVM-EL-03-02-05 STX HTS EVOM™ Electrode for TEER in MatTek 96

 

STX HTS compatible with EVOM, EVOM2™, and Millicell® ERS and ERS2

Order STX HTS EVOM™ Electrode for TEER from above and add Mini XLR to RJ-11 connector EVM-AC-02-01-01.

Order codes Replaces Historical Electrode
EVM-EL-03-03-05 & EVM-AC-02-01-01 STX100C96 - Electrode forEVOM2, Use with Corning HTS 96-Well Plate
EVM-EL-03-03-02 & EVM-AC-02-01-01 STX100M - Electrode for EVOM2, Use with Millipore Multiscreen HTS 96-Well Plate
EVM-EL-03-03-03 & EVM-AC-02-01-01 STX100F - Electrode for EVOM2, Use with Corning Falcon HTS Multi Well Insert System
EVM-EL-03-03-04 & EVM-AC-02-01-01 STX100C - Electrode for EVOM2, Use with Corning Costar HTS Transwell-24

 

Benefits

  • Smaller tip size than the STX4 electrode constructed for durability fits neatly into the keyhole shaped filter well
  • Electrode design reduces chance of contamination

Applications

  • STX HTS EVOM Electrodes are designed for TEER measurement in HTS culture plates using the EVOM Manual meters

Semi-permeable HTS (high throughput screening) culture plates have become a standard tool for pharmaceutical and institutional research in epithelial transport. HTS culture plates have well cups which are bonded together into a single assembly and are not removable. This makes the plates ideal for automated applications, but imposes a significant inconvenience when TEER must be measured in the absence of an automated system. WPI’s STX HTS EVOM series electrodes provide a cost-effective alternative to automation, allowing HTS well plates to be measured manually using a hand–held electrode.

The spatial orientation of an electrode during TEER measurement can have a significant effect on the resistance reading. When compared to the “chopsticks” electrode, the STX HTS EVOM series electrodes offer a technical advantage. The design of the STX HTS EVOM series electrodes guarantees spatial repeatability. Each STX HTS EVOM electrode is designed to self-align based on the form factor of the apical and basal access ports of the HTS plate. Each manufacturer of HTS culture systems has a unique form factor, and the correct STX HTS EVOM electrode to use is manufacturer specific.

WPI developed STX HTS EVOM electrodes for 24 and 96 well plates specific to Corning, Corning Falcon, Millipore and MatTek. Refer to the charts for compatibility information or contact your WPI sales representative for assistance. 

 

Compatibility charts

The STX HTS EVOM is compatible with the following Millpore plates.

Corning 96 

(EVM-EL-03-03-05)

Part # Description Pore Size Membrane
STX100C96

3380 HTS Transwell-96 System 1.0μm PET
3392  HTS Transwell-96 System 1.0μm PET
7369 HTS Transwell-96 System 0.4μm PET
3381 HTS Transwell-96 System 0.4μm PC
3391 HTS Transwell-96 System 0.4μm PC
3385 HTS Transwell-96 Well Plate 3.0μm PC
3386 HTS Transwell-96 Well Plate 3.0μm PC
3387 HTS Transwell-96 Well Plate 5.0μm PC
3388 HTS Transwell-96 Well Plate 5.0μm PC
3374 HTS Transwell-96 Well Plate 8.0μm PET
3384 HTS Transwell-96 Well Plate 8.0μm PET

Millipore 96 

(EVM-EL-03-03-02)

Part # Description Pore size Membrane


STX100M

PSHT004R1 96-well plate 0.4um PCF
PSRP004R1 96-well plate 1.0um PET
PSHT004R5 96-well plate 0.4um PCF
PSRP004R5 96-well plate 1.0um PET
PSHT004S5 96-well plate 0.4um PCF

Corning Biocoat/Falcon 24 

(EVM-EL-03-03-03)

Part # Description Pore Size Membrane

STX100F

 

351181 Corning Falcon HTS Multiwell Insert System 1.0μm PET  
351183 Corning Falcon HTS Multiwell Insert System 3.0μm PET
351185 Corning Falcon HTS Multiwell Insert System 8.0μm PET
354803 Corning BioCoat HTS Fibrillar Collagen Multiwell Insert System 1.0μm PET
354804 Corning BioCoat HTS Fibrillar Collagen Multiwell Insert System 1.0μm PC

Corning Costar 24 

(EVM-EL-03-03-04)

Part # Description  Pore Size  Membrane 

 

3378 HTS Transwell-24   0.4μm PET 
3379  HTS Transwell-24   0.4μm PET
3396  HTS Transwell-24  0.4μm  
3397 HTS Transwell-24  0.4μm  
3398 HTS Transwell-24  3.0μm  
3399 HTS Transwell-24  3.0μm   

MatTek 96 

(EVM-EL-03-02-05)

Part # Description Pore size Membrane

CCI96-PET-0.4 PermaCell Cell Culture Insert Plates, 96-well 0.4µm PET

Sample plates



 

This is a Corning #3378 24-well plate that may be used with the the EVM-EL-03-03-04.

 

The Corning Falcon 24-well plate is virtually identical to this one, and it may be used with the EVM-EL-03-03-03

This is a Millipore Multiscreen CaCo2 that may be used with the EVM-EL-03-03-02.  This is a Corning 3391 HTS Traswell 96 plate that may be used with the EVM-EL-03-03-05.


Choose Quantity
EVM-EL-03-02-05

EVM-EL-03-02-05

STX HTS EVOM™ Electrode for TEER in MatTek 96

  • Compatible with EVOM Manual and EVOM3 meters
  • For MatTek 96-well plates
  • Exclusively compatible with Evom Manual


Choose Quantity
EVM-EL-03-03-02

EVM-EL-03-03-02

STX HTS EVOM™ Electrode for EVOM Manual, use with Millipore Multiscreen HTS 96-Well Plate

  • For Millipore Multiscreen HTS 96-Well Plate
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01. (Original Manufacture Replacement for ERS-2 part MERSSTX00)


Choose Quantity
EVM-EL-03-03-03

EVM-EL-03-03-03

STX HTS EVOM™ Electrode for EVOM Manual, use with Corning Falcon HTS Multi Well Insert System

  • For Corning Falcon HTS Multi Well Insert System
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.


Choose Quantity
EVM-EL-03-03-04

EVM-EL-03-03-04

STX HTS EVOM™ Electrode for EVOM Manual, use with Corning Costar HTS Transwell-24

  • For Corning Costar HTS Transwell-24
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.
     


Choose Quantity
EVM-EL-03-03-05

EVM-EL-03-03-05

STX HTS EVOM™ Electrode for EVOM Manual, use with Corning HTS 96-Well Plate

  • For Corning HTS 96-Well Plate
  • Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
  • Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.


Choose Quantity
EVM-AC-02-01-01

EVM-AC-02-01-01

Mini XLR to RJ-11 Adapter Cable For Legacy TEER Meters

  • Enables TEER electrodes for EVOM™ Manual to be used with legacy meters including EVOM2, EVOM, EVOMX, ERS, ERS2 TEER instruments

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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