EVOM2
Trans Epithelial electric resistance (TEER) Measurements
- Overview
- Specifications
- Accessories
- Citations
- Related Products
Overview
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Instruction Manual
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Brochure
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Application Note
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NEWER Model Available - Check out the EVOM™ Manual HERE
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Non-destructively test for epithelial monolayer confluence in 2D cell cultures
- Measures trans-epithelial electrical resistance or trans-epithelilal voltage
- Compatible with 12 and 24 well culture plate systems out of the box
- Includes industry standard STX2 hand held “chopstick” electrodes
- Analog output for recording resistance or voltage measurements
- Auto ranging from 0-10 KΩ
- Battery powered
- Manual TEER measurement of epithelial cells in 6, 12, 24 and 96* well plates
- BNC output for data acquisition system
- Compatible with EndOhm chambers
- *96-well plate measurement requires an STX100 series electrode.
During the last two decades TEER measurements have become universally established as the most convenient, reliable and non-destructive method to evaluate and monitor the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. Recently there has been a significant surge of interest in introducing a combined electrode for resistance measurements in the Millipore 96-well PAMPA (parallel artificial membrane permeability assay) plate.
TEER measurement technology, which was first introduced by WPI in the mid-1980’s, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.
EVOM2™ Epithelial Voltohmmeter
- Manual TEER measurements of epithelial cells in 6-, 12-, and 24-well plates
- Electrically isolated meter that plugs into a standard outlet for continual readout without push buttons
- Compatible with Endohm chambers
- STX2 manual electrodes and test electrode included with every meter
- Free standing with tilt bail, making viewing results easy
- Standard analogue BNC output port
Benefits
- You can verify performance and calibrate the meter for TEER function using provided test resistor
- Battery powered meter is portable
- A variety of accessory electrodes are available for measuring TEER in 6- and 96-well fixed (HTS) and removable well culture systems (See STX100 series and Endohm electrodes)
Applications
- TEER and trans-epithelial voltage measurements in 2D cell cultures
Making TEER Measurements to Determine Cellular Confluence
The EVOM was the first instrument designed specifically to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. EVOM2™ is the next generation, redesigned for ease of use. The EVOM2™ not only qualitatively measures cell monolayer health, but also quantitatively measures cellular confluence. The unique electronic circuit of the EVOM2™ and the included STX2 electrode detect the confluence of the cellular monolayer. When combined with WPI’s Endohm chamber, the EVOM2™ can also be used to perform more accurate quantitative measurements or lower resistance measurements like transendothelial electrical resistance measurements.
Isolated battery power for 10 hours of use
The isolated power source of the EVOM2™ was specifically designed to avoid adverse effects on tissue and the formation of electrode metal deposits, even when it is plugged into a standard wall outlet. Now, the EVOM2™ is always on when you need it. In addition, its rechargeable battery allows up to 10 hours of mobile use.
Accurate reading every time
The four-and-a-half digit readout provides a range of 1-9,999 Ω. The included test electrode lets you calibrate the resistance measurements for an accurate reading every time. An analog BNC output is standard with the EVOM2™, providing an output port for recording data or remote display of the EVOM2™ output.
Electrode pair to measure voltage, pass current
EVOM2™ comes complete with the popular STX2 “chopstick” electrodes, 4 mm wide and 1 mm thick. Each stick of the electrode pair contains a silver/silver-chloride pellet for measuring voltage and a silver electrode for passing current. The small size of each electrode is designed to facilitate placement of the electrodes into a variety of standard cell culture wells.
STX2 STX3
The EVOM was the first instrument designed specifically to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. EVOM2 is the next generation, redesigned for ease of use. The EVOM2 not only qualitatively measures cell monolayer health, but also quantitatively measures cellular confluence. The unique electronic circuit of the EVOM2 and the included STX2 electrode detect the confluence of the cellular monolayer. When combined with WPI’s Endohm chamber, the EVOM2 can also be used to perform more accurate quantitative measurements or lower resistance measurements like trans endothelial electrical resistance measurements.
The isolated power source of the EVOM2 was specifically designed to avoid adverse effects on tissue and the formation of electrode metal deposits, even when it is plugged into a standard wall outlet. Now, the EVOM2 is always on when you need it. In addition, its rechargeable battery allows up to 10 hours of mobile use. The four and a half digit readout provides a range of 1-9,999 Ω. The included test electrode lets you calibrate the resistance measurements for an accurate reading every time, and the voltage meter never needs calibration. An analog BNC output is standard with the EVOM2, providing an output port for recording data or remote display of the EVOM2 output.
EVOM2 comes complete with the popular STX2 “chopstick” electrodes, 4 mm wide and 1mm thick. Each stick of the electrode pair contains a silver/silver-chloride pellet for measuring voltage and a silver electrode for passing current. The small size of each electrode is designed to facilitate placement of the electrodes into a variety of standard cell culture wells.
Other Resources
JOVE video: Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers. J. Vis. Exp. (80), e50638, doi:10.3791/50638 (2013) can be watched here.
VIDEO: Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
See how to use and EndOhm chamber with an EVOM2 meter.
In this video Mike shows you how to equilibrate your STX electrodes.
In this video, you can learn how to test your EVOM2.
Cellular Confluency of Epithelial Cells
The original EVOM was featured in this application video.
Specifications
Available models
Part # | Voltmeter | Electrode | Charger (800496) |
Battery (91736) |
1 kΩ Test Resistor (91750) |
EVOM2 | 0-10 kΩ Range Epithelial Volt/Ohm Meter |
STX2 (Basic Electrode) |
Yes | Yes (Installed) | Yes |
91799 | 0-10 kΩ Range Epithelial Volt/Ohm Meter |
STX3 |
Yes | Yes (Installed) | Yes |
300523 | 0-100 kΩ Range Epithelial Volt/Ohm Meter |
STX2
(Basic Electrode) |
Yes | Yes (Installed) | Yes |
EVOM2 specifications
MEMBRANE VOLTAGE RANGE | ±199.0 mV |
VOLTAGE MEASUREMENT | 0.1mV |
RESISTANCE RANGES | 0 to 9999 ohm |
RESISTANCE RESOLUTION | 1 ohm |
AC SQUARE WAVE CURRENT | ±10 µA at 12.5 Hz |
POWER | Internal rechargeable 6V NiMH 2200 mAH battery with external 12VDC supply for recharging |
SHIPPING WEIGHT | 3 lb (1.4 Kg) |
ELECTRODE CONNECTION | RJ-11 Connector (telephone style) |
TEST RESISTOR | External |
ENVIRONMENTAL RANGE | 10-38 C (50-100 F) 0 - 90% non-condensing relative humidity |
Accessories
EVM-EL-03-03-01
STX4 EVOM™ Electrode with Removable Blades for TEER in 6.5 mm Inserts
STX100C
DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Corning Costar HTS ...
STX100C96
DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Corning HTS 96-Well...
STX100F
DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Corning Falcon HTS ...
STX100M
DISCONTINUED - STX HTS EVOM™ Electrode for EVOM2, use with Millipore Multiscre...
EVM-EL-03-01-03
ENDOHM-24 EVOM™ Electrode for TEER in 24 mm Insert, for use with EVOM Manual a...
EVM-EL-03-01-02
ENDOHM-12 EVOM™ Electrode for TEER in 12 mm Insert, for use with EVOM Manual a...
STX2
Chopstick Electrode Set for EVOM2, 4mm
(Original Manufacture Direct Replacemen...
STX3
Chopstick Electrode Set for EVOM2
(Original Manufacture Direct Replacement for...
Citations
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