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EVOM™ Manual

EVOM™ Manual Epithelial Volt Ohm Meter for TEER Measurement

 

The EVOM™ Manual replaces all previously manufactured WPI manual TEER meters, including the EVOM3, EVOM2, and MilliCell® ERS-2, which have now been discontinued.

The EVOM™ Manual delivers improved workflow efficiency, more stable and repeatable measurements versus traditional Trans Epithelial Electrical Resistance (TEER) meters. Providing users with vital feedback during experiment measurements, the EVOM™ Manual’s large screen offers a range of informational views. The new graphical displays for trend analysis and measurement values helps scientists deliver simple, stepwise methodology during experimental measurements. The touch screen interface provides you with an intuitive, easy-to-use menu for configuration.

Eliminating the need to log data by hand, the EVOM™ Manual features automatic data logging for streamlined data collection. When used with the footswitch it enables handsfree recording of measurements

At the heart of the EVOM™ Manual is our latest processor and circuitry, providing users with quick, easy and reliable readings due to its fast stabilization, automatic twenty times sampling average and low noise design. The auto ranging resistance feature allows for fast resistance measurements, and an over-range display feature eliminates false readings. The EVOM™ Manual has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistance ranges up to 100 KΩ.

NEW FEATURE:  In addition to the existing capability of data storage on a USB flash drive, the new version of the EVOM™ Manual now provides an option for a more secured mode of data transfer using a Windows® companion application.

Features

• Low noise design offers greater resolution and accuracy in your TEER measurement protocol
• Automatic 20X sample averaging improves accuracy and stability
• Adjustable fixed measurement currents (2, 4 or 10 μA)
• Resistance auto ranging from 1 Ω to 100,000 Ω or with three fixed current ranges
• Reliable low current, low voltage design prevents metal ion transport
• Fast resistance stabilization on low levels under 200 Ω with resolution to 0.1 Ω
• Ergonomic tilt stand for low glare operation
• Graphical display of popular plates (6, 12, 24, 96) for trend analysis
• Display shows the most recent set of parameters
• Automatic plate indexing operation with or without control well subtraction for resistance and potential difference (PD) measurements
• Continuous data logging via USB (PC, Mac, Linux)
• Saves date stamped data to a spreadsheet readable file on a USB drive
• Upgradable firmware

Benefits of the EVOM™ Manual TEER Measurement Device

• Eliminates errors and reduces experimental processing time
• Auto data logging eliminates the need to track data by hand
• The small footprint allows more bench space
• Easy calibration and verification
• Footswitch for hands-free recording
• Prevent data loss with auto save and data recovery when battery is low
• Easy TEER value calculation by applying a unit area formula to the resistance

Applications for TEER Assays

• Measure epithelial or endothelial tissues for confluence, TEER and potential difference
• Permeability, conductance and drug studies
• Continuous digital monitoring of a target membrane
• Common studies
• Blood-brain barrier transport
• Lung epithelial tissue studies
• Intestinal tissue studies
• Skin studies

How Does the EVOM™ Manual Work?

Confluence of a cellular monolayer is determined by an increase or a plateau in tissue resistance detected using the unique electronic circuit of the EVOM™ Manual and the new STX4 electrode. The EVOM™ Manual qualitatively measures cell monolayer health and quantitatively measures cell confluence. The EVOM™ Manual produces a low AC current that avoids electrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. The EVOM™ Manual uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluence in cell cultures. In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. The accuracy and repeatability of the EVOM™ Manual-STX4 system makes this instrument ideal for permeability, PD and other detailed membrane studies.

Electrodes For TEER (Epithelial) Measurement

Part #	Descriptions
EVM-EL-03-03-01	STX4 EVOM™ Electrode with Removable Blades for TEER in 6 mm Inserts
STX2*	Replacement Electrode Set (Requires 99672 for use with the EVOM3 or EVOM™ Manual)
STX3*	Adjustable electrode set for shallow wells, 5-9 mm depth
3993*	2 mm Adapter for EVOM2

*(Requires 99672 for use with the EVOM3 or EVOM™ Manual)

ENDOHM Chambers For Endothelial/Epithelial Measurement

NEW EndOhm chambers include the EVOM™ Manual cable 99916.

Part #	Descriptions
EVM-EL-03-01-01	ENDOHM-6 EVOM™ Electrode for TEER in 6 mm Insert
EVM-EL-03-01-02	ENDOHM-12 EVOM™ Electrode for TEER in 12 mm Insert
EVM-EL-03-01-03	ENDOHM-24 EVOM™ Electrode for TEER in 24 mm Insert

TEER measurement techniques for in vitro barrier model systems

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