MCJ1-400
MechanoCulture MCJ1 Standard (150 mL chamber) Cyclic Strain Bioreactor – Independent 6-Chamber specimen tension stimulation
A cyclic strain bioreactor with a reusable sample holder that includes six individual 150 mL chambers with large grips. Often selected for soft tissue mechanical stimulation studies and larger engineered constructs.
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Overview
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Brochure MCJ1
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Mechanoculture J1 Standard for up to 6 specimen tension stimulation (150mL chambers)
Mechanical Stimulation Bioreactor for Tissue and Substrate Stretching
The MechanoCulture J1 (MCJ1) cyclic strain bioreactor delivers programmable uniaxial stretching to tissues, scaffolds, and engineered constructs in six independent culture chambers. Each chamber has its own actuator and force transducer, enabling parallel stimulation with per-well protocol control. The MCJ1 is designed for incubator-based studies where repeatable cyclic strain stimulation supports mechanobiology experiments and tissue engineering workflows in vitro.
Specimens are mounted with grips in a reusable chamber plate with media, placed on the controller, and run inside the incubator using a saved stimulation protocol. The system can monitor the force-displacement data in order to determine the stiffness profile of each specimen as a function of time. An optional cooling pump can be added to limit actuator heat transfer during long runs.
Researchers commonly use the MCJ1 to apply cyclic strain to flexible substrates, scaffold-based constructs, and soft tissue specimens, then relate stiffness changes and biological outcomes across treatment conditions.
Key Features
- 6 independently-programmed test protocols
- 6 force/displacement data outputs with onboard data storage
- Load cell options: 10N, 20N, 50N, 100N
- 25mm actuator travel with grip separation up to 55mm
- Spring-closure specimen grips with adjustable gripping force
- Ports for fluid exchange in each well
- Autoclavable chambers
- Fluid-cooled incubator-compatible design
- PC-independent operation
- Software for specifying simple, cyclic, and intermittent stimulation protocols

What MechanoCulture J1 is designed for
- In vitro parallel mechanical stimulation of up to 6 specimens with independent protocol control per chamber
- Substrate stretching protocols for cell and tissue constructs, including intermittent and multi-phase stimulation sequences
- Experiments where force and displacement logging supports stiffness tracking across time points
- Mechanotransduction studies where consistent strain input is required across cohorts
What's Included
- Programming and data download software (lifetime license)
- Reusable cell culture sample holder with 6 individual chambers (150 mL volume)
- Controller for up to 100 N uniaxial tension stimulation
- Six load cells (10 N, 20 N, 50 N, or 100 N)
Videos
MCJ1 Introduction
Setting up a test with the MCJ1
Options

The size of culture wells can be customized to support the width and extended length of the specimens being studied. For narrow specimens, the chamber volume can be reduced to less than 20mL. For specimens up to 40mm wide with an extended length of 55mm, the chamber volume can be increased to 150mL.
Specimen Gripping
To securely grip the specimen without causing damage, the grips must have a suitable clamping surface and apply a consistent clamping force throughout the test.
Standard specimen grips have smooth clamping surfaces with squared front and back edges. Clamps with serrated, rough, and customized gripping surfaces are also available.
For most applications, a spring-closure grip preferred to apply a repeatable clamping force that does not significantly change with specimen thickness or deformation. Grips are available with spring stiffnesses that can deliver up to 150N of clamping force. Grip springs can be changed in the field as needed to successfully test a range of specimens. Screw-closure grips are also available.
| Grip Style | L1 | M1 | S1 | S2 |
| Close Mechanism | Spring | Spring | Spring | Screw |
| Max Clamping Force (N) | 120 | 55 | 20 | 150 |
| Grip Width (mm) | 40 | 19 | 12 | 10 |
| Chamber Volume (mL) | 150 | 60 | 30 | 20 |
The chambers, grips and associated screws, seals, and other components are made from corrosion-resistant materials that can be autoclave-sterilized.
Specifications
| Dimensions | 16.5 X 11.5 X 30 cm |
| Weight | 7 kg |
| Stimulation Mode | Customizable tension / cyclic strain |
| Number of Wells | 6 |
| Chamber Volume | 150 mL |
| Force Capacity | Up to 100 N |
| Available Load Cells | 10, 20, 50 and 100 N |
| Force Accuracy | 0.2% of load cell capacity |
| Maximum Stretch Displacement | 25.4 mm |
| Maximum Grip Seperation | 55 mm |
| Maximum Velocity | 4 mm/s |
| Maximum Cycle Frequency | 2 Hz |
Accessories
Citations
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