Microinjection Tool Box
The system depicted includes components often favored by researchers:
- MICRO-ePUMP Pneumatic PicoPump with built in MICRO-ePORE™ Cell Penetrator to facilitate microinjections,
- P-1000 Micropipette Puller
- M4C stand
- M3301R micromanipulator
- PZMTIII microscope with optional lighted base with articulating mirror and optional PRO-300 HDS camera and view screen
- E2XX micropipette storage jar
- Z-MOLDS Microinjection and Transplantation Molds
- 14003-G Vannas spring scissors
- Glass capillaries
- 77020 glass tweezers
- Fluorodish optical grade glass bottom culture dishes
Whatever your needs, WPI offers a range of equipment to fill your requirements.
Microinjection Systems Selection
Empowering scientists with reliable instruments
Serving scientists for over 50 years, WPI offers a variety of instruments for microinjection including pumps, pipetters, microscopes and more. One of our most popular pumps for microinjection is the PV850 Pneumatic PicoPump.
The PV850, PV830, uPump and MICRO-ePUMP, Pneumatic PicoPumps, were designed to simplify intracellular injection. You get repeatable microinjection in volumes ranging from picoliters to nanoliters. The volume injected is controlled by the inside diameter of the glass tip, the pressure and the time.
- The PV850 offers eject and hold pressure. The hold pressure prevents backfilling of the pipette by capillary action, ensuring repeatable injections.
- In addition, the PV830 also has vacuum pressure which allows you to securely hold a cell with one pipette while you inject it with another.
- The uPUMP and MICRO-ePUMP have a built in compressor eliminating the need for an external pressure supply. The MICRO-ePUMP with Integrated Cell Penetrator and Internal Pressure Source is perfect for intracellular injection. The Cell Penetrator delivers a highly localized voltage signal to a targeted injection site to facilitate penetration with minimal trauma.
WPI has a customizable Microinjection System with everything you need to get started. The basic system is shown here. Below you will find many options and accessories you may use to customize your system.
Options
You may customize your system using the following options:
MICROSCOPE
- PZMIII Precision Stereo Zoom Microscope on Track Stand
- PZMIV Precision Stereo Zoom on Track Stand
- PZMIII-MI Stereo Microscope with LED Illuminated Base and Articulating Mirror
- 504928 LED Lighted Microscope Stand, 12.5"
- 504929 LED Lighted Microsccope Stand, 10.5"
- 504596 Halogen Lighted Microscope Stand
INJECTOR
Microinjectors Data Sheet
- PV850 Pneumatic PicoPump with Hold Pressure
- PV830 Pneumatic PicoPump with Hold Pressure and Vacuum
- uPump Pneumatic PicoPump with built in compressor
- MICRO-ePUMP with built in compressor and MICRO-ePORE™ Cell Penetrator to facilitate microinjections
- UMPIII UltraMicroPump
- Nanoliter2020 Injector
MANIPULATOR
PULLER
- PUL-1000 Programmable Pipette Puller
- P-30 Vertical Pipette Puller
- P-97 Flaming/Brown Pipette Puller
- P-1000 Micropipette Puller
Accessories
- 801566/801963 Vacuum Pump for use with the PV830
- Nanofil Microliter syringes
- MicroFil for backfilling glass needles
- MICRO-ePORE
- PRO-300HDS HD Camera with screen
- F1 LED-Light-Source
- Z-LITE-186 Fiber optic illuminator with (500186) Bifrucate Light Guides
- FluoroDish Optical glass bottom dishes
- Glass capillaries
- Z-molds microinjection and transplantation molds
- Pipetters
- MicroTip pre-pulled pipettes
- E2XX Micropipette Storage Jar
- 504134 LED Ring light
- M10 Manipulator stand
- M-3 Tiilting manipulator base
- Many surgical instruments, including:
- 77020 Glass tweezers
- 500342 Dumont Tweezers #5
- 504507 Fine pointed Swiss tweezers
- 14003 Stainless Steel Vannas spring scissors
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