How to Stabilize EVOM™ Auto GxP Electrodes

WPI’s EVOM™ Auto simplifies transepithelial electrical resistance (TEER) measurement by automating measurements in 24- and 96-well plates. A GxP module powered by TotalLab is available to meet the FDA’s 21 CFR Part 11 compliance regulations. Before making measurements, you need to stabilize your electrodes. See how here.

1. Select Maintenance from the main menu. The Maintenance window appears
2. To stabilize, press the + or – buttons to set the timer to the desired time in minutes in the Stabilize frame
3. Select the radio button for the rinse station containing the buffer solution
4. Fill the appropriate rinse station with a buffer solution
5. Press the Start Timer button. The Start Stabilize popup displays. Enter your comment and your EVOM™ Auto GxP Client password. If you don’t want to add a comment, you may select the No Comment checkbox. If you do, default text appears in the comment line. Then, press the Start button. The time interval displays on the counter in the center of the window. The title changes to Stabilizing Time Remaining
6. The electrode array moves to the selected rinse station, and the counter starts counting down to zero
7. When the count reaches zero, the electrode array retracts
8. At any point, you may stop the counter by pressing the Stop Timer button. This resets the counter to zero and retracts the electrode array.