How To Stabilize The Electrode Array On The EVOM™ Auto

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.

The video below shows you how to stabilize the electrode array on the EVOM™ Auto:

First, select Maintenance on the main menu. To stabilize, press the + or - buttons to set the timer to the desired time in minutes in the stablize frame.

Next, Select the radio button for the rinse station containing the buffer solution. Then, fill the appropriate rinse station with the buffer solution. Press the Start Timer button. The time interval displays on the counter in the center of the window. The title also changes to "Stabilizing Time Remaining".

The electrode array moves to the selected rinse station, and the counter begins counting down to zero. When the count reaches zero, the electrode array retracts. 

At any point, you may stop the counter by pressing the Stop Timer button. This resets the counter to zero and retracts the electrode array.

NOTE: The radio button selection requires different rinse stations to be used for chloriding and stabilizing.