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MICRO-ePORE Accessories

MICRO-ePORE Accessories

MICRO-ePORE™ Electrode Holders

These electrode holders are designed exclusively for use the with MICRO-ePORE™ system, which includes the silver wire electrode holders. The platinum wire electrode holders are an optional accessory. Platinum wire electrode holders are important when you are working with proteins. The silver chloride wire is perfect for work with RNA. However, the silver chloride wire tends to denature proteins, reducing the efficiency of the microinjection solution.

MICRO-ePORE™ Electrode Holders Options

 Order code Description Details Kit includes
300683 WPI MICRO-ePORE Holder, Silver Wire Microelectrode holder designed for use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a WPI PV850 or uPUMP injector. (1) PicoNozzle tip assemblies (Handle diameter is 6.25 mm and 100 mm long.)
(1) 5’ tubing
(2) 1.0 mm pipette gaskets (lime green)
(2) 1.2 mm pipette gaskets (black)
(2) 1.5 mm pipette gaskets (red)
(2) 1.65 mm pipette gaskets (white)
(2) sealing gaskets
300683PT WPI MICRO-ePORE Holder, Platinum Wire Microelectrode holder designed for use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a WPI PV850 or uPUMP injector. (1) PicoNozzle tip assemblies (Handle diameter is 6.25 mm and 100 mm long.)
(1) 5’ tubing
(2) 1.0 mm pipette gaskets (lime green)
(2) 1.2 mm pipette gaskets (black)
(2) 1.5 mm pipette gaskets (red)
(2) 1.65 mm pipette gaskets (white)
(2) sealing gaskets
300684 Femtojet MICRO-ePORE Holder, Silver Wire Microelectrode holder designed for use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Eppendorf Femtojet® injector. (2) 1.0 mm pipette gaskets (lime green)
(2) 1.2 mm pipette gaskets (black)
(2) 1.5 mm pipette gaskets (red)
(2) 1.65 mm pipette gaskets (white)
(2) sealing gaskets
300684PT Femtojet MICRO-ePORE Holder, Platinum Wire Microelectrode holder designed for use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Eppendorf Femtojet® injector. (2) 1.0 mm pipette gaskets (lime green)
(2) 1.2 mm pipette gaskets (black)
(2) 1.5 mm pipette gaskets (red)
(2) 1.65 mm pipette gaskets (white)
(2) sealing gaskets
300685 Narishige MICRO-ePORE Holder, Silver Wire Microelectrode holder designed for use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Narishige injector. (2) 1.0 mm pipette gaskets (lime green)
(2) 1.2 mm pipette gaskets (black)
(2) 1.5 mm pipette gaskets (red)
(2) 1.65 mm pipette gaskets (white)
(2) sealing gaskets
300685PT Narishige MICRO-ePORE Holder, Platinum Wire Microelectrode holder designed for use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Narishige injector. (2) 1.0 mm pipette gaskets (lime green)
(2) 1.2 mm pipette gaskets (black)
(2) 1.5 mm pipette gaskets (red)
(2) 1.65 mm pipette gaskets (white)
(2) sealing gaskets

 

Applications for Silver Wire Electrode Holders

Silver wire electrodes are suitable for most applications.

Applications for Platinum Wire Electrode Holders

Initially CRISPR-Cas9 technology started with RNA antisense sequences, but it is now moving towards proteins. Using platinum electrodes offers more active insertion of the genetic material capability, and there is a better chance of changing the genome. Some common protein applications for the platinum wire electrode holders include:

  • crRNA contains the guide RNA that locates the correct segment of host DNA along with a region that binds to tracrRNA (generally in a hairpin loop form), forming an active complex.
  • tracrRNA binds to crRNA and forms an active complex.
  • sgRNA (single-guide RNA) is a combined RNA consisting of a tracrRNA and at least one crRNA.
  • Cas9 (most common) involves an enzyme whose active form is able to modify DNA. Many variants exist with different functions (i.e. single-strand nicking, double-strand breaking, DNA binding) due to each enzyme's DNA site recognition function.
  • Repair Template is a DNA molecule used as a template in the host cell's DNA repair process, allowing insertion of a specific DNA sequence into the host segment broken by Cas9.

Femtojet® 4I is a registered Trademark of Eppendorf AG

Reference Electrodes

Probe-Style Reference Electrode For WPI MICRO-ePORE™

For the probe-style electrode, the black pin of the microelectrode interface cable connects to the back side of the reference electrode block. The probe-style electrode pin plugs into the front of the block. Place the block of the probe style reference electrode near the injection site. Then, bend the bare reference wire as needed to make contact with the liquid medium in order to complete the circuit.

  • For use with the MICRO-ePORE™
  • Probe style reference electrode with platinum wire
  • Second option reference electrode

 

Well-Style Reference Electrode For WPI MICRO-ePORE™

The reference electrode ring is secured to a microscope slide with a vacuum gel, and the media is placed inside the well-style electrode. Both the probe-style electrode and the well-style electrode are included with the MICRO-ePORE™ system. This is a replacement part. [Ring reference electrode – OD: 12.7 mm (0.5”), Thickness: 0.4 mm (0.16”)].

  • For use with the MICRO-ePORE™
  • Well style reference electrode ring
  • OD: 12.7 mm (0.5”)
  • Thickness: 0.4 mm (0.16”)


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300683PT

300683PT

WPI MICRO-ePORE Holder, Platinum Wire Kit

  • Platinum wire
  • For use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a WPI PV850 or uPUMP injector
  • Kit includes: (1) PicoNozzle tip assemblies (Handle diameter is 6.25 mm and 100 mm long), (1) 5’ tubing, (2) 1.0 mm pipette gaskets (lime green), (2) 1.2 mm pipette gaskets (black), (2) 1.5 mm pipette gaskets (red), (2) 1.65 mm pipette gaskets (white) and (2) sealing gaskets


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300683

300683

WPI MICRO-ePORE Holder, Silver Wire Kit

  • Silver wire
  • For use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a WPI PV850 or uPUMP injector
  • Kit includes: (1) PicoNozzle tip assemblies (Handle diameter is 6.25 mm and 100 mm long), (1) 5’ tubing, (2) 1.0 mm pipette gaskets (lime green), (2) 1.2 mm pipette gaskets (black), (2) 1.5 mm pipette gaskets (red), (2) 1.65 mm pipette gaskets (white) and (2) sealing gaskets


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300684PT

300684PT

Femtojet MICRO-ePORE Holder, Platinum Wire Kit

  • Platinum wire
  • For use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Femtojet 4i or Femtojet 4x injector
  • Kit includes: (2) 1.0 mm pipette gaskets (lime green), (2) 1.2 mm pipette gaskets (black), (2) 1.5 mm pipette gaskets (red), (2) 1.65 mm pipette gaskets (white) and (2) sealing gaskets


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300684

300684

Femtojet MICRO-ePORE Holder, Silver Wire Kit

  • Silver wire
  • For use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Femtojet 4i or Femtojet 4x injector
  • Kit includes: (2) 1.0 mm pipette gaskets (lime green), (2) 1.2 mm pipette gaskets (black), (2) 1.5 mm pipette gaskets (red), (2) 1.65 mm pipette gaskets (white) and (2) sealing gaskets


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300685PT

300685PT

Narishige MICRO-ePORE Holder, Platinum Wire Kit

  • Platinum wire
  • For use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Narishige injector
  • Kit includes: (2) 1.0 mm pipette gaskets (lime green), (2) 1.2 mm pipette gaskets (black), (2) 1.5 mm pipette gaskets (red), (2) 1.65 mm pipette gaskets (white) and (2) sealing gaskets


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300685

300685

Narishige MICRO-ePORE Holder, Silver Wire Kit

  • Silver wire
  • For use with the WPI MICRO-ePORE Pinpoint Cell Penetrator when using a Narishige injector
  • Kit includes: (2) 1.0 mm pipette gaskets (lime green), (2) 1.2 mm pipette gaskets (black), (2) 1.5 mm pipette gaskets (red), (2) 1.65 mm pipette gaskets (white) and (2) sealing gaskets


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99192

99192

Probe-style Reference Electrode For MICRO-ePORE™

  • For use with the MICRO-ePORE™
  • Probe style reference electrode with platinum wire


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99190

99190

Well-style Reference Electrode For MICRO-ePORE™

  • For use with the MICRO-ePORE™
  • Well style reference electrode ring
  • OD: 12.7 mm (0.5”)
  • Thickness: 0.4 mm (0.16”)


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13142

13142

Foot Switch for the MICRO2T, MICRO-ePORE and EVOM3 (13142)


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75122-110

75122-110

PicoNozzle Gasket, 1.0mm, Green 10/pk

  • 1.0 mm hole size 
  • For use with the PV820, PV830, PV850, MICRO-ePORE, MICRO-ePUMP, UPUMP MMP, DMP


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75122-210

75122-210

PicoNozzle Gasket, 1.2mm, Black 10/pk

  • 1.2 mm hole size 
  • For use with the PV820, PV830, PV850, MICRO-ePORE, MICRO-ePUMP, UPUMP MMP, DMP


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75122-310

75122-310

PicoNozzle Gasket, 1.5mm, Red 10/pk

  • 1.5 mm hole size 
  • For use with the PV820, PV830, PV850, MICRO-ePORE, MICRO-ePUMP, UPUMP MMP, DMP


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75122-410

75122-410

PicoNozzle Gasket, 1.65mm, White 10/pk

  • 1.65 mm hole size 
  • For use with the PV820, PV830, PV850, MICRO-ePORE, MICRO-ePUMP, UPUMP MMP, DMP

Featured Publication

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration.
[Using WPI's UMP3-1]

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