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FluoroDish Cell Culture

Optical Grade Glass Bottom Culture Dishes For Imaging

Cover-glass bottom for observing and growing cells for imaging

• Optical quality glass bottom on petri dish offers better imaging quality (RI=1.525)
• Low sample volume for expensive chemicals
• Flat bottom optimizes heat exchange
• Less optical distortion and superior UV transmission
• Low cytotoxicity adhesive to ensure cells' survival
• Supplied individually packed and gamma sterilized in a box of 100
• Coated dishes available for better cell adhesion
• Blackwall dishes are available to block stray lights and reduce background noise

 

Improve Research Results

with FluoroDish Optical Grade Glass Bottom Culture Dishes

FluoroDish Brochure

Get the highest quality images and video for your research with FluoroDish Cell Culture dishes. Their optical quality glass bottom is as thin as a coverslip, which ensures the least amount of distortions and excellent heat transfer without any of the autofluoresence issues so common with plastic petri dishes.

Benefits

When you need quality glass bottom cell culture dishes with less optical distortion, excellent UV transmission, low cytotoxicity and guaranteed sterility, order WPI FluoroDishes. 

• Optical quality glass bottom of the petri dish for shorter working distances, larger numerical aperture and higher magnification
• Non-fluorescent glass so you can discern weaker signals
• Allows the use of immersion objectives
• Flat bottom optimizes heat exchange
• Less optical distortion and superior UV transmission
• Low cytotoxicity adhesive to ensure cells' survival
• Individually packaged and gamma sterilized
• Multiple sizes and designs to suit your application
• Optional black wall to minimize background fluorescence and light scattering
• Optional coatings used to improve cell adhesion, growth, and differentiation
• Dishes designed for low volumes or large growth areas

Optical Grade Glass Bottom for Exceptional Imaging Quality 

FluoroDish™ dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection, and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish™ uses a specially formulated adhesive that is optically clear and durable with extremely low toxicity. The bottom glass has superior UV transmission: 30% transmission at 300 nm, compared to less than 7% for the most popular German glass. Stringent quality control ensures that glass thickness stays within the 0.17 ±0.01 mm range. Cited in 575 reference articles (in the NIH PubMed Central® alone), our optical grade, glass bottom culture dishes are unique in the marketplace and conform to strict quality control standards.

Specially Formulated Low Toxicity Adhesive

FluoroDish™ optical glass bottom dishes use a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour survival rate of rat embryos typically exceeds 95% when kept in a FluoroDish™. 

Multiple sizes

We have a 50 mm diameter petri dish and two types of 35 mm diameter petri dishes. An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. They are individually packed, and gamma sterilized.

The 35 mm petri dish has outside dimensions similar to that of a Corning 35 mm petri dish and has ø23.5 mm glass window (FD35) or ø10 mm glass window (FD3510). Most heaters and perfusion adapters designed for the Corning 35 mm petri dish also fit this petri dish.

Clear Glass Bottom Dishes

Conventional plastic dishes and chambers limit the utility of the inverted scope for many applications, as thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17mm thick) optical quality glass bottom, allowing the use of a much shorter objective working distance, larger numerical aperture (NA), and a higher magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with a medium such as water, glycerin, or oil for the highest magnification possible. To optimize heat exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

Coated Glass Bottom Dishes

Coated FluoroDish™ are used to improve cell adhesion, growth, and differentiation within the optical glass bottom culture dish.

WPI offers a range of coated 35mm dishes, including Collagen, Fibronectin, Poly-D-Lysine, Poly-L-Lysine, and Vitronectin. Coated culture dishes are useful in promoting cell adhesion to culture surface and can be used for cell attachment, cell expansion, serum-free cultures, and cancer research. Additionally, coated dishes allow for detailed observation of cellular process when doing high-resolution live cell imaging analysis. When doing cell adhesion assays, coated dishes facilitate studying cell-substrate interations. Coated culture dishes are suitable for microinjection experiments, too.

• Collagen is often used to promote the adhesion and growth of various cell types, including fibroblasts, endothelial cells, and epithelial cells
• Poly-L-Lysine enhances cell adhesion for neurons, glial cells, and other cell types
• Fibronectin is a high-molecular-weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins
• Vitronectin is a glycoprotein found in the extracellular matrix and blood plasma
• Poly-D-Lysine utilized in experiments involving primary neuronal cultures and cell adhesion supports neurite outgrowth on PDL coated optical glass. 

Black Wall Glass Bottom Dishes

WPI’s Blackwall FluoroDishes™ have low background fluorescence, making them ideal for live cell microscopy, as well as fluorescence microscopy, other high-resolution microscopy techniques, confocal microscopy, and inverted microscopy. They are also a good choice for applications like Total Internal Reflection Fluorescence (TIRF) and super-resolution microscopy. When working in a dark room, the black dishes allow for better visualization of the meniscus of the media. Blackwall FluoroDishes™ have a low rim and sidewall, making them suitable for micromanipulation or microinjection experiments.

Low Volume Glass Bottom Dishes Or Large Growth Areas

The 10 mm glass dish (FD3510) has low sidewalls for easy microelectrode access and low solution volume. The low microelectrode access angle is the lowest among all of 35 mm dishes on the market (very close to that of a 50 mm dish). The dish needs only about 115 µL to cover the bottom well, an important feature when using expensive drugs and chemicals.

The 50 mm petri dish (FD50) has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling.

Options

Order Code	Description	Coating	Wall Colour
FD35-100	FluoroDish, Glass Bottom, Clear Wall, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	None	Clear
FD3510-100	FluoroDish, Glass Bottom, Clear Wall, 35 mm, 10 mm Well, Low Sidewall, Individually Packed, Sterile, 100/bx	None	Clear
FD5040-100	FluoroDish, Glass Bottom, Clear Wall, 50 mm, 35 mm Well, Individually Packed, Sterile, 100/bx	None	Clear
FD35B-100	FluoroDish, Glass Bottom, Black Wall, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	None	Black
FD3510B-100	FluoroDish, Glass Bottom, Black Wall, 35 mm, 10 mm Well, Low Sidewall, Individually Packed, Sterile, 100/bx	None	Black
FD5040B-100	FluoroDish, Glass Bottom, Black Wall, 50 mm, 35 mm Well, Individually Packed, Sterile, 100/bx	None	Black
FD35PDL-100	FluoroDish, Glass Bottom, Poly-D-Lysine Coated, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	Poly-D- Lysine	Clear
FD35COL-100	FluoroDish, Glass Bottom, Collagen Coated, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	Collagen	Clear
FD35FN-100	FluoroDish, Glass Bottom, Fibronectin Coated, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	Fibronectin	Clear
FD35PLL-100	FluoroDish, Glass Bottom, Poly-L-Lysine Coated, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	Poly-L- Lysine	Clear
FD35VN-100	FluoroDish, Glass Bottom, Vitronectin Coated, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx	Vitronectin	Clear

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