MF200-2

Overview

• Simple, reliable, and economically priced
• Fabricate glass micropipettes
• Analog temperature controller
• W30S-LED microscope included

Applications

The MF200 Microforge is a versatile instrument designed specifically for the fabrication of glass micropipettes and other related tools. The system was developed in collaboration with Dr. Ming Li of the Department of Pharmacology, University of South Alabama. It is perfect for patch pipette tip polishing, tip size reduction, contact stretching, in vitro fertilization pipette production and a variety of other pipette configurations. The MF200 simple, reliable and is priced economically.

Features

The MF200 system includes: An easy to use analog temperature controller, a specially configured WPI model W30S-LED research grade compound microscope, 40x long-working distance objective and 10x eyepiece. 40x magnification is essential when polishing pipettes as small as half a micron (0.5 µm) in diameter. Compared to a conventional 40x objective, the long working distance objective reduces the danger of damage to the pipette and/or objective lens during the polishing process. It is also the only commercial microforge using the Kohler illuminator and Abbe condenser for illumination. This provides less glare and sharper image of the pipette than frosted glass illuminator, which was used on all of the other commercial Microforge.

Specifications

AC POWER MODULE	100-240 VAC 50/60 Hz
FILAMENTS (3)	H2, H3, H4
FILAMENT ON	Pushbutton Controlled or Optional Foot Switch Controlled
FILAMENT ADJUSTMENT ASSEMBLY	For 40x and 25x Long-Working, Distance Objectives: mounts on objective
FILAMENT ADJUSTMENT ASSEMBLY: OBJECTIVE	40x Long-Working Distance (3 mm)
FILAMENT ADJUSTMENT ASSEMBLY: OPTIONAL	25x Long-Working Distance (5 mm)
EYEPIECE	10x (pair)
EYEPIECE: RETICLE (10x eyepiece only)	1.25 µm/division (at 40x), 0-90º Angle at 5º/division
EYEPIECE: OPTIONAL EYEPIECE	15x (pair)
GLASS HOLDER	Mounts on Microscope Stage
DIMENSIONS: Control Unit	4 x 7 x 1.875in. (10.2 x 17.8 x 4.8 cm)
SHIPPING WEIGHT	3 lb. (1.4 kg)
MICROSCOPE:	See W30S-LED
MICROSCOPE: SHIPPING WEIGHT	16 lb. (7.3 kg)

Accessories

Citations

Kadeba, P., Vasauskas, A., & Chen, H. (2013). Regulation of store-operated calcium entry by FK506-binding immunophilins. Cell calcium. Retrieved from https://www.sciencedirect.com/science/article/pii/S0143416012002126

Koselski, M., Trebacz, K., & Dziubinska, H. (2013). Cation-permeable vacuolar ion channels in the moss Physcomitrella patens: a patch-clamp study. Planta. Retrieved from https://link.springer.com/article/10.1007/s00425-013-1902-4

Kupisz, K., Trebacz, K., & Gruszecki, W. (2014). Menthol-induced action potentials in Conocephalum conicum as a result of unspecific interactions between menthol and the lipid phase of the plasma membrane? Physiologia plantarum. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/ppl.12288/abstract

Li, Z., Huang, H., & Yang, B. (2013). Amino acid substitutions in the pore affect the anomalous mole fraction effect of CaV1. 2 channels. Molecular  …. Retrieved from https://www.spandidos-publications.com/mmr/7/2/571?text=abstract

Lu, X., Deckey, R., Jiao, G., Ren, H., & Li, M. (2013). Caribbean maitotoxin elevates  i and activates non-selective cation channels in HIT-T15 cells. World journal of diabetes. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680626/

Nguyen, H., Badie, N., & McSpadden, L. (2014). Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells in Micropatterned Cell Pairs. Cardiac Tissue  …. Retrieved from https://link.springer.com/protocol/10.1007/978-1-4939-1047-2_21

Pawlikowska-Pawlega, B., & Dziubinska, H. (2014). Characteristics of quercetin interactions with liposomal and vacuolar membranes. …  et Biophysica Acta (BBA …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0005273613002915

Shepherd, A., Loo, L., & Mohapatra, D. (2013). Chemokine co-receptor CCR5/CXCR4-dependent modulation of Kv2. 1 channel confers acute neuroprotection to HIV-1 glycoprotein gp120 exposure. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0076698

Vasauskas, A., Chen, H., Wu, S., & Cioffi, D. (2014). The serine-threonine phosphatase calcineurin is a regulator of endothelial store-operated calcium entry. Pulmonary Circulation. Retrieved from https://www.jstor.org/stable/10.1086/675641

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