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Dr Andy MacKenzie, University of the West of Scotland

I have had dealings with WPI for several years and have consistently found their customer service to be second-to-none. They have always provided fast and insightful responses to any technical query and such support has been of enormous help to my laboratory.
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Micropipette Holders

Micropipette Holders

WPI’s microelectrode holder-half-cells couple fluid-filled glass micropipettes to high input impedance amplifiers.


Micropipette Holders and Half Cells

WPI’s microelectrode holder-half-cells couple fluid-filled glass micropipettes to high input impedance amplifiers. A Ag/AgCl pellet (or a silver wire) molded into the holder body provides stable potential. Electrical connection is made via male 2mm pins or female 2mm sockets. The pipette may be mounted axially or at right angles to the holder. Pipettes are held with screw-caps or rubber gaskets (without caps). Filling WPI microelectrode holders with electrolytes containing chloride results in stable electrode potential. Suitable electrolytes include KCl, NaCl andCaCl2. Holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0mm outside diameters. (Call WPI regarding custom designs for other glass diameters.) The holder style you select will depend on your experimental application, space, and instrumentation.

Hints for selecting and ordering micropipette holders

  1. Determine the required electrical connection on the holder. For example, if you wish to connect the holder to a 2mm pin, you should select a holder equipped with a 2mm jack. Most WPI probes require a holder equipped with a 2mm jack.
  2. Decide on the required alignment of the electrical connection (in-line with the glass pipette or at a right angle to it). Space considerations in your experimental setup and requirements imposed by other pieces of equipment typically determine which alignment is appropriate.
  3. Determine if you want to hold the glass pipette with a rubber gasket (MEH1S) or a screw-cap (MEH3S). Rubber gaskets offer easier insertion and removal of glass pipettes whereas screw-caps provide more secure mounts for micropipetters.
  4. Choose a holder with either a silver wire or a silver/silver chloride pellet for the metal/liquid coupling. Silver/silver chloride pellets provide a more stable, low-noise baseline which is important for low-noise DC recording. Pellets require the glass pipette and holder to be free of air bubbles to achieve a good connection. Silver wire holders are durable and are easier to use when the holder is equipped with a pressure port because the fluid in the pipette does not have to be filled to the top of the pipette to achieve a good electrical connection.
  5. Choose a holder equipped with a pressure port only when you want to pressure inject liquid from the pipette. Two types of ports are available: 2.0mm O.D. and standard “syringe-style” luer. The luer port is often recommended because it makes assembly and disassembly much easier. Quick-connect luer fittings for four common sizes of tubing (1/16", 3/32", 1/8", 5/32" I.D.) are included with each luer-equipped holder.
  6. Some non-WPI preamplifiers or headstages cannot be mounted on micromanipulators. In such cases, a holder equipped with a rod (MEH8) permits the holder to be conveniently mounted on a micromanipulator.
  7. Finally, remember to specify the O.D. of the glass you will be using when you place your order.

Keep in mind these general principles when considering the micropipettes:

  • MEH6RF/SF is designed primarily for use with the Model 900A Micropressure System.
  • EHB1 is for use in electrode beveling.
  • MEH3SW is for microtitration of chloride with a silver wire as the electrode and a solution of silver nitrate filling the holder.
  • MPH models do not contain Ag/AgCl half-cells and are used for pressure injection of substances through microelectrodes.
  • PicoNozzle, used for pressure injection with PV800 Series PicoPumps, includes an MPH6S holder — which may also be used to couple a micropipette to a syringe.
 Order code Custom Order Electric Connection Angle Connector Half-Cell Pressure Port Screw Cap Designed for WPI Products
EHB1
EHB1
  Straight Male Wire No Port N/A MBS, 48000
EHBF
EHBF  
  Straight Female Wire No Port N/A MBS, 48000
FOIMPH
FOIMPH
  Straight Fiber Optic None No Port w/Cap MBS, 48000
MBMPH5-7
MBMPH5-7
  ---- None None Female Luer w/Cap  
MEH145
MEH145
Yes 45° Male Pellet No Port No Cap  
MEH1F45 
MEH145F
Yes 45° Female Pellet No Port No Cap 705, 773, 767, 721, FD223
MEH1R
MEH1R
  Right Male Pellet No Port No Cap  
MEH1RF
MEH1RF
 Yes Right Female Pellet No Port No Cap 705, 773, 767, 721, FD223
MEH1S
MEH1S
  Straight Male Pellet No Port No Cap  
MEH1SF
MEH1SF
  Straight Female Pellet No Port No Cap 705, 773, 767, 721, FD223
MEH2R
MEH2R
 Yes Right Male Pellet Male Luer w/Cap  
MEH2RF
MEH2RF
 Yes Right Female Pellet Male Luer w/Cap 705, 773, 767, 721, FD223
MEH2RFW
MEH2RFW
 Yes Right Female Wire Male Luer w/Cap 705, 773, 767, 721, FD223
MEH2RW
MEH2RW
 Yes Right Male Wire Male Luer w/Cap  
MEH2S
MEH2S
  Straight Male Pellet Male Luer w/Cap  
MEH2SF
MEH2SF
  Straight Female Pellet Male Luer w/Cap 705, 773, 767, 721, FD223
MEH2SFW
MEH2SFW
 Yes Straight Female Wire Male Luer w/Cap 705, 773, 767, 721, FD223
MEH2SW
MEH2SW
  Straight Male Wire Male Luer w/Cap  
MEH345
MEH345
  45° Male Pellet No Port w/Cap  
MEH3F45
MEH3F45
 Yes 45° Female Pellet No Port w/Cap 705, 773, 767, 721, FD223
MEH3FW45
MEH3FW45
 Yes 45° Female Wire Port w/Cap  
MEH3R
MEH3R
  Right Male Pellet No Port w/Cap  
MEH3RF
MEH3RF
  Right Female Pellet No Port w/Cap 705, 773, 767, 721, FD223
MEH3RFW
MEH3RFW
 Yes Right Female Wire No Port w/Cap 705, 773, 767, 721, FD223
MEH3RW
MEH3RW
 Yes Right Male Wire No Port w/Cap  
MEH3S
MEH3S
  Straight Male Pellet No Port w/Cap  
MEH3SBW
MEH3SBW
 Yes Straight Banana Wire No Port w/Cap ISO-80, ISO-DAM8A
MEH3SF
MEH3SF
  Straight Female Pellet No Port w/Cap 705, 773, 767, 721, FD223
MEH3SFW
MEH3SFW
  Straight Female Wire No Port w/Cap 705, 773, 767, 721, FD223
MEH3SW
MEH3SW
  Straight Male Wire No Port w/Cap  
MEH3W45
MEH3W45
 Yes 45° Male Wire No Port w/Cap 705, 773, 767, 721, FD223
MEH6RF
MEH6RF
  Right Female Pellet 2.0-mm Port w/Cap 705, 773, 767, 721, FD223
MEH6RFW
MEH6RFW
 Yes Right Female Wire 2.0-mm Port w/Cap 705, 773, 767, 721, FD223
MEH6SF
MEH6SF
  Straight Female Pellet 2.0-mm Port w/Cap 705, 773, 767, 721, FD223
MEH6SFW
MEH6SFW
 Yes Straight Female Wire 2.0-mm Port w/Cap 705, 773, 767, 721, FD223
MEH7
MEH7
  Right Male Pellet 2.0-mm Port w/Cap  
MEH7W
MEH7W
 Yes Right Male Wire 2.0-mm Port w/Cap  
MEH8
MEH8
  Right Male Pellet No Port w/Cap  
MEH900R
MEH900R
  Right Male Pellet 2.0-mm Port w/Cap 900A
MEH900S
MEH900S
  Straight Male Pellet 2.0-mm Port w/Cap 900A
MPH1
MPH1
  None None Female Luer w/Cap  
MPH3
MPH3
  None None Male Luer w/Cap  
MPH4
MPH4
  None None 2.0-mm Port w/Cap  
MPH6P
MPH6P
Yes Right Male Pellet Female Luer w/Cap Piconozzle Kit (5430-XX)
MPH6R
MPH6R
Yes Right Male Wire Female Luer w/Cap Piconozzle Kit (5430-XX)
MPH6S
MPH6S
  None None Female Luer w/Cap Piconozzle Kit (5430-XX)


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APEH1

APEH1

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, 2 Screw Caps. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order. APEH models are designed for use in pressurized injection procedures.


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APEH2

APEH2

Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, 2 Screw Caps. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order. APEH models are designed for use in pressurized injection procedures.


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EHB1

EHB1

Microelectrode Holder, straight, Male Connector, Wire Half-Cell, No Pressure Port. For use with these WPI Products: MBS, 48000. EBH1 is designed for use in electrode beveling and fits capillary glass from 1.0 to 2.0 mm outer diameters.


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EHBF

EHBF

Microelectrode Holder, straight, Female Connector, Wire Half-Cell, No Pressure Port. For use with these WPI Products: MBS, 48000. EBHF is designed for use in electrode beveling and fits capillary glass from 1.0 to 2.0 mm outer diameters.


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FOIMPH

FOIMPH

Microelectrode Holder, straight, Fiber Optic Connector, No Pressure Port, Screw Cap. For use with these WPI Products: MBS, 48000. FOIMPH is designed for use in electrode beveling and fits capillary glass from 1.0 to 2.0 mm outer diameters.


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MEH145

MEH145

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, No Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH1F45

MEH1F45

Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, No Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH1R

MEH1R

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, No Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH1RF

MEH1RF

Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, No Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH1S

MEH1S

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, No Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH1SF

MEH1SF

Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, No Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2R

MEH2R

Microelectrode Holder, Male Connector, Pellet Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2RF

MEH2RF

Microelectrode Holder, Female Connector, Pellet Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2RFW

MEH2RFW

Microelectrode Holder, Female Connector, Wire Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2RW

MEH2RW

Microelectrode Holder, Male Connector, Wire Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2S

MEH2S

Microelectrode Holder, Male Connector, Pellet Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2SF

MEH2SF

Microelectrode Holder, Female Connector, Pellet Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2SFW

MEH2SFW

Microelectrode Holder, Female Connector, Wire Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH2SW

MEH2SW

Microelectrode Holder, Male Connector, Wire Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH345

MEH345

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3F45

MEH3F45

Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3FW45

MEH3FW45

Microelectrode Holder, Female Connector, Wire Half-Cell, Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3R

MEH3R

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3RF

MEH3RF

Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3RFW

MEH3RFW

Microelectrode Holder, Female Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3RW

MEH3RW

Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3S

MEH3S

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3SBW

MEH3SBW

Microelectrode Holder, Banana Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: ISO-80, ISO-DAM8A. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3SF

MEH3SF

Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3SFW

MEH3SFW

Microelectrode Holder, Female Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3SW

MEH3SW

Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH3W45

MEH3W45

Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH6RF

MEH6RF

Microelectrode Holder, Female Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH6RFW

MEH6RFW

Microelectrode Holder, Female Connector, Wire Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH6SF

MEH6SF

Microelectrode Holder, Female Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH6SFW

MEH6SFW

Microelectrode Holder, Female Connector, Wire Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH7

MEH7

Microelectrode Holder, Male Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH7W

MEH7W

Microelectrode Holder, Male Connector, Wire Half-Cell, 2.0mm Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH8

MEH8

Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH900R

MEH900R

Microelectrode Holder, Male Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-900A. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MEH900S

MEH900S

Microelectrode Holder, Male Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-900A. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH1

MPH1

Microelectrode Holder, Female Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH3

MPH3

Microelectrode Holder, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH4

MPH4

Microelectrode Holder, 2.0mm Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH6P

MPH6P

Microelectrode Holder, Male Connector, Pellet Half-Cell, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH6R

MPH6R

Microelectrode Holder, Male Connector, Wire Half-Cell, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH6S

MPH6S

Microelectrode Holder, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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2505

2505

Electrode Handle 6.3 mm. Microelectrode holder handle for use with WPI manipulators.


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5444

5444

Electrode Handle 4.8 mm. Microelectrode holder handle for use with Narishige and Zeiss manipulators.


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GO1-100

GO1-100

Replacement Gasket 1.0mm. Use with microelectrode holders. 100 per package.


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GO2-100

GO2-100

Replacement Gasket 1.2mm. Use with microelectrode holders. 100 per package.


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GO3-100

GO3-100

Replacement Gasket 1.5mm. Use with microelectrode holders. 100 per package.


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GO4-100

GO4-100

Replacement Gasket 2.0mm. Use with microelectrode holders. 100 per package.


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EH-P170

EH-P170

Polycarbonate pipette holder 1.0-1.7 mm O.D.

Compatible with HL-U and all Axon/Molecular headstages

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