Micropipette Holders
WPI’s microelectrode holder-half-cells couple fluid-filled glass micropipettes to high input impedance amplifiers.
Micropipette Holders and Half Cells
WPI’s microelectrode holder-half-cells couple fluid-filled glass micropipettes to high input impedance amplifiers. A Ag/AgCl pellet (or a silver wire) molded into the holder body provides stable potential. Electrical connection is made via male 2mm pins or female 2mm sockets. The pipette may be mounted axially or at right angles to the holder. Pipettes are held with screw-caps or rubber gaskets (without caps). Filling WPI microelectrode holders with electrolytes containing chloride results in stable electrode potential. Suitable electrolytes include KCl, NaCl andCaCl2. Holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0mm outside diameters. (Call WPI regarding custom designs for other glass diameters.) The holder style you select will depend on your experimental application, space, and instrumentation.
Hints for selecting and ordering micropipette holders
- Determine the required electrical connection on the holder. For example, if you wish to connect the holder to a 2mm pin, you should select a holder equipped with a 2mm jack. Most WPI probes require a holder equipped with a 2mm jack.
- Decide on the required alignment of the electrical connection (in-line with the glass pipette or at a right angle to it). Space considerations in your experimental setup and requirements imposed by other pieces of equipment typically determine which alignment is appropriate.
- Determine if you want to hold the glass pipette with a rubber gasket (MEH1S) or a screw-cap (MEH3S). Rubber gaskets offer easier insertion and removal of glass pipettes whereas screw-caps provide more secure mounts for micropipetters.
- Choose a holder with either a silver wire or a silver/silver chloride pellet for the metal/liquid coupling. Silver/silver chloride pellets provide a more stable, low-noise baseline which is important for low-noise DC recording. Pellets require the glass pipette and holder to be free of air bubbles to achieve a good connection. Silver wire holders are durable and are easier to use when the holder is equipped with a pressure port because the fluid in the pipette does not have to be filled to the top of the pipette to achieve a good electrical connection.
- Choose a holder equipped with a pressure port only when you want to pressure inject liquid from the pipette. Two types of ports are available: 2.0mm O.D. and standard “syringe-style” luer. The luer port is often recommended because it makes assembly and disassembly much easier. Quick-connect luer fittings for four common sizes of tubing (1/16", 3/32", 1/8", 5/32" I.D.) are included with each luer-equipped holder.
- Some non-WPI preamplifiers or headstages cannot be mounted on micromanipulators. In such cases, a holder equipped with a rod (MEH8) permits the holder to be conveniently mounted on a micromanipulator.
- Finally, remember to specify the O.D. of the glass you will be using when you place your order.
Keep in mind these general principles when considering the micropipettes:
- MEH6RF/SF is designed primarily for use with the Model 900A Micropressure System.
- EHB1 is for use in electrode beveling.
- MEH3SW is for microtitration of chloride with a silver wire as the electrode and a solution of silver nitrate filling the holder.
- MPH models do not contain Ag/AgCl half-cells and are used for pressure injection of substances through microelectrodes.
- PicoNozzle, used for pressure injection with PV800 Series PicoPumps, includes an MPH6S holder — which may also be used to couple a micropipette to a syringe.
| Order code | Custom Order | Electric Connection Angle | Connector | Half-Cell | Pressure Port | Screw Cap | Designed for WPI Products |
|---|---|---|---|---|---|---|---|
| EHB1 |
Straight | Male | Wire | No Port | N/A | MBS, 48000 | |
| EHBF |
Straight | Female | Wire | No Port | N/A | MBS, 48000 | |
| FOIMPH |
Straight | Fiber Optic | None | No Port | w/Cap | MBS, 48000 | |
| MBMPH5-7 |
---- | None | None | Female Luer | w/Cap | ||
| MEH145 |
Yes | 45° | Male | Pellet | No Port | No Cap | |
| MEH1F45 |
Yes | 45° | Female | Pellet | No Port | No Cap | 705, 773, 767, 721, FD223 |
| MEH1R |
Right | Male | Pellet | No Port | No Cap | ||
| MEH1RF |
Yes | Right | Female | Pellet | No Port | No Cap | 705, 773, 767, 721, FD223 |
| MEH1S |
Straight | Male | Pellet | No Port | No Cap | ||
| MEH1SF |
Straight | Female | Pellet | No Port | No Cap | 705, 773, 767, 721, FD223 | |
| MEH2R |
Yes | Right | Male | Pellet | Male Luer | w/Cap | |
| MEH2RF |
Yes | Right | Female | Pellet | Male Luer | w/Cap | 705, 773, 767, 721, FD223 |
| MEH2RFW |
Yes | Right | Female | Wire | Male Luer | w/Cap | 705, 773, 767, 721, FD223 |
| MEH2RW |
Yes | Right | Male | Wire | Male Luer | w/Cap | |
| MEH2S |
Straight | Male | Pellet | Male Luer | w/Cap | ||
| MEH2SF |
Straight | Female | Pellet | Male Luer | w/Cap | 705, 773, 767, 721, FD223 | |
| MEH2SFW |
Yes | Straight | Female | Wire | Male Luer | w/Cap | 705, 773, 767, 721, FD223 |
| MEH2SW |
Straight | Male | Wire | Male Luer | w/Cap | ||
| MEH345 |
45° | Male | Pellet | No Port | w/Cap | ||
| MEH3F45 |
Yes | 45° | Female | Pellet | No Port | w/Cap | 705, 773, 767, 721, FD223 |
| MEH3FW45 |
Yes | 45° | Female | Wire | Port | w/Cap | |
| MEH3R |
Right | Male | Pellet | No Port | w/Cap | ||
| MEH3RF |
Right | Female | Pellet | No Port | w/Cap | 705, 773, 767, 721, FD223 | |
| MEH3RFW |
Yes | Right | Female | Wire | No Port | w/Cap | 705, 773, 767, 721, FD223 |
| MEH3RW |
Yes | Right | Male | Wire | No Port | w/Cap | |
| MEH3S |
Straight | Male | Pellet | No Port | w/Cap | ||
| MEH3SBW |
Yes | Straight | Banana | Wire | No Port | w/Cap | ISO-80, ISO-DAM8A |
| MEH3SF |
Straight | Female | Pellet | No Port | w/Cap | 705, 773, 767, 721, FD223 | |
| MEH3SFW |
Straight | Female | Wire | No Port | w/Cap | 705, 773, 767, 721, FD223 | |
| MEH3SW |
Straight | Male | Wire | No Port | w/Cap | ||
| MEH3W45 |
Yes | 45° | Male | Wire | No Port | w/Cap | 705, 773, 767, 721, FD223 |
| MEH6RF |
Right | Female | Pellet | 2.0-mm Port | w/Cap | 705, 773, 767, 721, FD223 | |
| MEH6RFW |
Yes | Right | Female | Wire | 2.0-mm Port | w/Cap | 705, 773, 767, 721, FD223 |
| MEH6SF |
Straight | Female | Pellet | 2.0-mm Port | w/Cap | 705, 773, 767, 721, FD223 | |
| MEH6SFW |
Yes | Straight | Female | Wire | 2.0-mm Port | w/Cap | 705, 773, 767, 721, FD223 |
| MEH7 |
Right | Male | Pellet | 2.0-mm Port | w/Cap | ||
| MEH7W |
Yes | Right | Male | Wire | 2.0-mm Port | w/Cap | |
| MEH8 |
Right | Male | Pellet | No Port | w/Cap | ||
| MEH900R |
Right | Male | Pellet | 2.0-mm Port | w/Cap | 900A | |
| MEH900S |
Straight | Male | Pellet | 2.0-mm Port | w/Cap | 900A | |
| MPH1 |
— | None | None | Female Luer | w/Cap | ||
| MPH3 |
— | None | None | Male Luer | w/Cap | ||
| MPH4 |
— | None | None | 2.0-mm Port | w/Cap | ||
| MPH6P |
Yes | Right | Male | Pellet | Female Luer | w/Cap | Piconozzle Kit (5430-XX) |
| MPH6R |
Yes | Right | Male | Wire | Female Luer | w/Cap | Piconozzle Kit (5430-XX) |
| MPH6S |
— | None | None | Female Luer | w/Cap | Piconozzle Kit (5430-XX) |
APEH1
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, 2 Screw Caps. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order. APEH models are designed for use in pressurized injection procedures.
APEH2
Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, 2 Screw Caps. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order. APEH models are designed for use in pressurized injection procedures.
EHB1
Microelectrode Holder, straight, Male Connector, Wire Half-Cell, No Pressure Port. For use with these WPI Products: MBS, 48000. EBH1 is designed for use in electrode beveling and fits capillary glass from 1.0 to 2.0 mm outer diameters.
EHBF
Microelectrode Holder, straight, Female Connector, Wire Half-Cell, No Pressure Port. For use with these WPI Products: MBS, 48000. EBHF is designed for use in electrode beveling and fits capillary glass from 1.0 to 2.0 mm outer diameters.
FOIMPH
Microelectrode Holder, straight, Fiber Optic Connector, No Pressure Port, Screw Cap. For use with these WPI Products: MBS, 48000. FOIMPH is designed for use in electrode beveling and fits capillary glass from 1.0 to 2.0 mm outer diameters.
MEH145
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, No Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH1F45
Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, No Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH1R
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, No Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH1RF
Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, No Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH1S
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, No Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH1SF
Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, No Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2R
Microelectrode Holder, Male Connector, Pellet Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2RF
Microelectrode Holder, Female Connector, Pellet Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2RFW
Microelectrode Holder, Female Connector, Wire Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2RW
Microelectrode Holder, Male Connector, Wire Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2S
Microelectrode Holder, Male Connector, Pellet Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2SF
Microelectrode Holder, Female Connector, Pellet Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2SFW
Microelectrode Holder, Female Connector, Wire Half-Cell, Male Luer, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH2SW
Microelectrode Holder, Male Connector, Wire Half-Cell, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH345
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3F45
Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3FW45
Microelectrode Holder, Female Connector, Wire Half-Cell, Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3R
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3RF
Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3RFW
Microelectrode Holder, Female Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3RW
Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3S
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3SBW
Microelectrode Holder, Banana Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: ISO-80, ISO-DAM8A. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3SF
Microelectrode Holder, Female Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3SFW
Microelectrode Holder, Female Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3SW
Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH3W45
Microelectrode Holder, Male Connector, Wire Half-Cell, No Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH6RF
Microelectrode Holder, Female Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH6RFW
Microelectrode Holder, Female Connector, Wire Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH6SF
Microelectrode Holder, Female Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH6SFW
Microelectrode Holder, Female Connector, Wire Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-705, SYS-773, 767, 721, FD223. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH7
Microelectrode Holder, Male Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH7W
Microelectrode Holder, Male Connector, Wire Half-Cell, 2.0mm Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH8
Microelectrode Holder, Male Connector, Pellet Half-Cell, No Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH900R
Microelectrode Holder, Male Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-900A. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MEH900S
Microelectrode Holder, Male Connector, Pellet Half-Cell, 2.0mm Pressure Port, Screw Cap. For use with these WPI Products: SYS-900A. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MPH1
Microelectrode Holder, Female Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MPH3
Microelectrode Holder, Male Luer, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MPH4
Microelectrode Holder, 2.0mm Pressure Port, Screw Cap. All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MPH6P
Microelectrode Holder, Male Connector, Pellet Half-Cell, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MPH6R
Microelectrode Holder, Male Connector, Wire Half-Cell, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
MPH6S
Microelectrode Holder, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.
2505
Electrode Handle 6.3 mm. Microelectrode holder handle for use with WPI manipulators.
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5444
Electrode Handle 4.8 mm. Microelectrode holder handle for use with Narishige and Zeiss manipulators.
GO1-100
Replacement Gasket 1.0mm. Use with microelectrode holders. 100 per package.
GO2-100
Replacement Gasket 1.2mm. Use with microelectrode holders. 100 per package.
GO3-100
Replacement Gasket 1.5mm. Use with microelectrode holders. 100 per package.
GO4-100
Replacement Gasket 2.0mm. Use with microelectrode holders. 100 per package.
EH-P170
Polycarbonate pipette holder 1.0-1.7 mm O.D.
Compatible with HL-U and all Axon/Molecular headstages
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