MS-50M-01

Overview

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MicroSquisher Brochure
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The MicroSquisher is designed to perform compression testing on specimens between 50 and 2000µm with force resolutions as small as 0.05µN.

A micro-scale tension/compression test system

The MicroSquisher does what others can’t. Smaller specimens, better force resolution, easier test setups, and great visuals. It operates on the same principles as an atomic force microscope – cantilever mechnanics – but at much larger scale. Applications are diverse but include small tissue samples, hydrogel microspheres, cell spheroids, and engineered tissue growth matrix.

The MicroSquisher’s setup, operation and data collection software module allows for simple execution of standard or customized test protocols. Test parameters such as displacement magnitudes, durations and data/image collection rates are specified in a table format for quick access and modification. A template system is used to quickly reload the desired test parameters once a protocol has been established.

While the test is running, the software provides real-time results graphing and a live video feed to facilitate user monitoring of the test progress. Ask anyone who has used our equipment and they will be quick to tell you how intuitive and useful this software package really is.

Specimens & Mounting

Uncompressed and compressed hydrogel microsphere. Original diameter: 3mm Compression: 40%
Unstretched and stretched gel scaffold Original size: 3.3mm wide, 1.6mm thick Extension: 40% Mounting via 5 puncture pins at 0.7mm spacing Modulus was 490Pa
Compressed air bubble Approximate size: 0.6mm	Compressed cell spheroid Approximate size: 500μm

 Videos

MicroScale Mechanical Testing Examples

Mechanical Testing of Soft Gels using the CellScale MicroSquisher

Muscle Tissue Construct Testing at Kent State University

CellScale MicroSquisher Instructional Overview

Compression Test of a Hydrogel Microsphere

Hydrogel Tension Test

  Zebrafish Embryo Cell Spheroid Compression Testing

Specifications

Accessories

Citations

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Gillies, D., Gamal, W., & Downes, A. (2017). Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy. Of SPIE Vol. http://doi.org/10.1117/12.2251492

Hached, F., Vinatier, C., Pinta, P.-G., Hulin, P., Le Visage, C., Weiss, P., … Grimandi, G. (2017). Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International, 2017, 1–11. http://doi.org/10.1155/2017/9303598

Henry, N., Clouet, J., Fragale, A., Griveau, L., Chédeville, C., Véziers, J., … Le Visage, C. (2017). Pullulan microbeads/Si-HPMC hydrogel injectable system for the sustained delivery of GDF-5 and TGF-β1: new insight into intervertebral disc regenerative medicine. Drug Delivery, 24(1), 999–1010. http://doi.org/10.1080/10717544.2017.1340362

Kasukonis, B. M., Kim, J. T., Washington, T. A., & Wolchok, J. C. (2016). Development of an infusion bioreactor for the accelerated preparation of decellularized skeletal muscle scaffolds. Biotechnology Progress, 32(3), 745–755. http://doi.org/10.1002/btpr.2257

Kvasnytsia, M., Famaey, N., Böhm, M., & Verhoelst, E. (2016). Patient Specific Vascular Benchtop Models for Development and Validation of Medical Devices for Minimally Invasive Procedures. Journal of Medical Robotics Research, 1(3), 1640008. http://doi.org/10.1142/S2424905X16400080

Mironov, V., & Yu, H. (2017). Tensiometric estimation of material properties of tissue spheroids. Retrieved from http://dc.engconfintl.org/biofab_tissue_model/18/

Monaco, L. A., DeWitte-Orr, S. J., & Gregory, D. E. (2016). A comparison between porcine, ovine, and bovine intervertebral disc anatomy and single lamella annulus fibrosus tensile properties. Journal of Morphology, 277(2), 244–251. http://doi.org/10.1002/jmor.20492

Park, D. W., Sebastiani, A., Yap, C. H., Simon, M. A., & Kim, K. (2016). Quantification of Coupled Stiffness and Fiber Orientation Remodeling in Hypertensive Rat Right-Ventricular Myocardium Using 3D Ultrasound Speckle Tracking with Biaxial Testing. PLOS ONE, 11(10), e0165320. http://doi.org/10.1371/journal.pone.0165320

Pradhan, S., Clary, J. M., Seliktar, D., & Lipke, E. A. (2017). A three-dimensional spheroidal cancer model based on PEG-fibrinogen hydrogel microspheres. Biomaterials, 115, 141–154. http://doi.org/10.1016/J.BIOMATERIALS.2016.10.052

Pradhan, S., Hassani, I., Seeto, W. J., & Lipke, E. A. (2017). PEG-fibrinogen hydrogels for three-dimensional breast cancer cell culture. Journal of Biomedical Materials Research Part A, 105(1), 236–252. http://doi.org/10.1002/jbm.a.35899

Seeto, W. J., Tian, Y., Winter, R. L., Caldwell, F. J., Wooldridge, A. A., & Lipke, E. A. (2017). Encapsulation of Equine Endothelial Colony Forming Cells in Highly Uniform, Injectable Hydrogel Microspheres for Local Cell Delivery. Tissue Engineering Part C: Methods, 23(11), 815–825. http://doi.org/10.1089/ten.tec.2017.0233

Silva, K. R., Rezende, R. A., Pereira, F. D. A. S., Gruber, P., Stuart, M. P., Ovsianikov, A., … Mironov, V. (2016). Delivery of Human Adipose Stem Cells Spheroids into Lockyballs. PLOS ONE, 11(11), e0166073. http://doi.org/10.1371/journal.pone.0166073

Sivakumaran, D., Mueller, E., & Hoare, T. (2017). Microfluidic production of degradable thermoresponsive poly( N -isopropylacrylamide)-based microgels. Soft Matter, 13(47), 9060–9070. http://doi.org/10.1039/C7SM01361B

Stewart, D. M., Monaco, L. A., & Gregory, D. E. (2017). The aging disc: using an ovine model to examine age-related differences in the biomechanical properties of the intralamellar matrix of single lamellae. European Spine Journal, 26(1), 259–266. http://doi.org/10.1007/s00586-016-4603-4

Wu, S., Wang, Y., Streubel, P., & Duan, B. (2017). Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation. Acta Biomaterialia. Retrieved from http://www.sciencedirect.com/science/article/pii/S1742706117305573

Yu, C., Kornmuller, A., Brown, C., Hoare, T., & Flynn, L. E. (2017). Decellularized adipose tissue microcarriers as a dynamic culture platform for human adipose-derived stem/stromal cell expansion. Biomaterials, 120, 66–80. http://doi.org/10.1016/J.BIOMATERIALS.2016.12.017

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