World Precision Instruments

Search the site by KEYWORD


Quick Finder


Testimonials

Researcher, University of Bristol

Our lab was interested in purchasing a syringe pump from WPI. The company's support was helpful in identifying the required model and even informed us of model-specific limitations (which were of no concern for our intended use). The overall processing and delivery was exceedingly fast – less tha...
read more

P-97

P-97

Flaming/Brown Pipette Puller from Sutter

  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

P-97

There are 1 images available to view - click to enlarge and scroll through the product gallery.

P-97 Datasheet
/ Download as PDF

  • Environmental chamber.
  • Programmable air pressure.
  • Memory storage for up to 100 programs.
  • Write protection and date stamp for each program.
  • Two symmetrical pipettes with each pull.
  • Pre-programmed sample programs for intracellular and patch pipettes.
  • Special programming on request.
  • Ramp test—self test for establishing program heat settings when a new filament or glass is introduced.
  • Vacuum fluorescent display.
  • Internal memory test.
  • Constant current power supply for filament and pull solenoid.
  • Looping pull cycle for fabrication of patch type micropipettes.
  • Self-contained air supply with filtration system and humidity control.
  • Consistent and reliable electrodes with tip diameters less than 0.1µm.
  • Control over the time and pressure at which the air is delivered.
  • Optimized velocity sensing circuit for maximized sensitivity and reproducibility.
  • Quality control, SEM photograph of a tip pulled with each puller; criterion is tip measurement less than 0.1µm and typically is ~0.06µm.


The Model P-97 micropipette puller is the latest generation of the Flaming/Brown type puller for fabrication of micropipettes, patch pipettes and microinjection needles. While retaining many of the features on the Model P-87, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. The P-97 combines a proven mechanical system with a sophisticated, programmable microprocessor controller. This programmable control of the pulling parameters allows the investigator to design application specific pipettes from a wide range of glass compositions and sizes.

A number of new features have been incorporated in the design of the P-97. One of the most apparent is the environmental chamber which houses the area surrounding the heating filament. The environmental chamber is designed to minimize the effect of changing humidity on the reproducibility of pulled pipettes. A 25% increase in power over the previous version allows for the use of larger heating filaments, larger diameter glass and multi-barrelled glass. The metal jaws that clamp the heating filament have also been redesigned to minimize heat retention. A gas delivery mode switch provides for extended cooling for large diameter and multi-barreled glass. A spring-loaded clamping mechanism has been added for easier loading of glass. A vacuum fluorescent display has been added that allows easy viewing from any direction.

New software improvements on the P-97 include a display of the total time that the heat is on to assist in program development and troubleshooting. Up to 100 programs can now be written and stored in memory, which makes the P-97 suitable for multiple users. These programs can now be write-protected, adding security to prevent programs from being changed or altered inadvertently. The display shows the last date and time the program was written or edited. In addition, the air pressure is included as a programmable parameter.

The P-97 contains a ramp test to overcome the difficulties of program adjustment when a new filament is installed or to help characterize a new glass and filament combination. This test allows for the rapid adjustment of heat values in established programs and provides estimates for establishing heat settings when using new sizes or compositions of glass capillaries. As with all Sutter instruments, the electronics have been carefully designed to maximize noise rejection.


NOTE: Not currently for sale to our customers in Germany and Italy - contact us for information 

Specifications

Accessories


Choose Quantity
FB320B

FB320B

3mm Box Filament, 2mm Wide

View details...


Choose Quantity
FB330B

FB330B

3mm Box Filament, 3mm Wide

View details...


Choose Quantity
FT345B

FT345B

Trough Filament, 4.5mm Wide

View details...


Choose Quantity
FB215B

FB215B

2mm Box Filament,1.5mm Wide

View details...


Choose Quantity
FB220B

FB220B

2mm Box Filament, 2mm Wide

View details...


Choose Quantity
FB230B

FB230B

2mm Box Filament, 3mm Wide

View details...


Choose Quantity
FB245B

FB245B

2.5mm Box Filament, 4.5mm Wide

View details...


Choose Quantity
FB255B

FB255B

2.5mm Box Filament, 2.5mm Wide

View details...


Choose Quantity
FB315B

FB315B

3mm Box Filament, 1.5mm Wide

View details...


Choose Quantity
FT320B

FT320B

Trough Filament, 2mm Wide

View details...


Choose Quantity
FT330B

FT330B

Trough Filament, 3mm Wide

View details...


Choose Quantity
FT315B

FT315B

Trough Filament, 1.5mm Wide

View details...

Citations

Beqollari, D., & Kammermeier, P. (2013). The interaction between mGluR1 and the calcium channel Ca< sub> V2. 1preserves coupling in the presence of long Homer proteins. Neuropharmacology. Retrieved from https://www.sciencedirect.com/science/article/pii/S0028390812002365

Beronja, S., & Fuchs, E. (2013). RNAi-Mediated Gene Function Analysis in Skin. Molecular Dermatology. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-227-8_23 

Bhattarai, J., Park, S., & Han, S. (2013). Potentiation of NMDA Receptors by Withania somnifera on Hippocampal CA1 Pyramidal Neurons. The American journal of Chinese …. Retrieved from https://www.worldscientific.com/doi/abs/10.1142/S0192415X13500365 

Chin, P., & Linden, M. van der. (2013). Enhanced luminescence of Ag nanoclusters via surface modification. …. Retrieved from https://iopscience.iop.org/0957-4484/24/7/075703 

Dilgen, J. (2013). Amygdala inputs drive feedforward inhibition in the medial prefrontal cortex. Journal of neurophysiology. Retrieved from https://jn.physiology.org/content/early/2013/04/12/jn.00531.2012.abstract 

Dryanovski, D., & Guzman, J. (2013). Calcium Entry and α-Synuclein Inclusions Elevate Dendritic Mitochondrial Oxidant Stress in Dopaminergic Neurons. The Journal of …. Retrieved from https://www.jneurosci.org/content/33/24/10154.short 

Edds-Walton, P., Matos, S., & Fay, R. (2013). Does the magnocellular octaval nucleus process auditory information in the toadfish, Opsanus tau? Journal of Comparative Physiology …. Retrieved from https://link.springer.com/article/10.1007/s00359-013-0799-5 

Fischer, M. (2013). Disrupting Sensitization of Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammatory Hyperalgesia. The Journal of …. Retrieved from https://www.jneurosci.org/content/33/17/7407.short 

Hao, J., Ruel, J., Coste, B., Roudaut, Y., Crest, M., & Delmas, P. (2013). Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons. Ion Channels. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-351-0_12 

Ho, C., Chiang, C., Lee, C., Fan, Y., & Su, C. (2013). Glutamatergic activities in neonatal rat spinal cord heterogeneously regulate single-fiber splanchnic nerve discharge. Autonomic Neuroscience. Retrieved from https://www.sciencedirect.com/science/article/pii/S1566070213000945 

Inoue, M., Tanimoto, M., & Oda, Y. (2013). The role of ear stone size in hair cell acoustic sensory transduction. Scientific reports. Retrieved from https://www.nature.com/srep/2013/130702/srep02114/full/srep02114.html 

Jusuf, P., Harris, W., & Poggi, L. (2013). Preparation of transgenic zebrafish embryos for imaging the developing retina. Cold Spring Harbor Protocols. Retrieved from https://cshprotocols.cshlp.org/content/2013/3/pdb.prot073536.short 

Kanak, D., Rose, G., Zaveri, H., & Patrylo, P. (2013). Altered Network Timing in the CA3-CA1 Circuit of Hippocampal Slices from Aged Mice. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0061364 

Kloet, A. de, Pati, D., & Wang, L. (2013). Angiotensin Type 1a Receptors in the Paraventricular Nucleus of the Hypothalamus Protect against Diet-Induced Obesity. The Journal of …. Retrieved from https://www.jneurosci.org/content/33/11/4825.short 

Krivitsky, L., & Wang, J. (2013). Locomotion of microspheres for imaging and light focusing applications. arXiv preprint arXiv: …. Retrieved from https://arxiv.org/abs/1307.5595 

Lamacchia, M., & Clayton, J. (2013). Silencing of Genes and Alleles by RNAi in Anopheles gambiae. Malaria. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-026-7_11 

Malmersjö, S., & Rebellato, P. (2013). Neural progenitors organize in small-world networks to promote cell proliferation. Proceedings of the …. Retrieved from https://www.pnas.org/content/110/16/E1524.short 

Phelan, K., Shwe, U., & Abramowitz, J. (2013). Canonical transient receptor channel 5 (TRPC5) and TRPC1/4 contribute to seizure and excitotoxicity by distinct cellular mechanisms. Molecular …. Retrieved from https://molpharm.aspetjournals.org/content/83/2/429.short 

Romanenko, S. (2013). Comparison of the effects of millimeter wave irradiation, general bath heating, and localized heating on neuronal activity in the leech ganglion. SPIE …. Retrieved from https://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1656385 

Sun, Q., & Pilowsky, P. (2013). Microiontophoretic Study of Individual Neurons During Intracellular Recording. Stimulation and Inhibition of Neurons. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-233-9_8 

Szydlowski, S., & Dorocic, I. (2013). Target selectivity of feedforward inhibition by striatal fast-spiking interneurons. The Journal of …. Retrieved from https://www.jneurosci.org/content/33/4/1678.short 

Varela, J., Wang, J., & Cooper, D. (2013). The effects of behavioral control over stress on GABAergic spontaneous inhibitory postsynaptic currents in prefrontal cortical pyramidal neurons. F1000Research. Retrieved from https://f1000research.com/articles/2-104/v1 

Wright, V., & Castro-Gómez, F. (2013). Structural studies of metal ligand complexes by ion mobility-mass spectrometry. International Journal for …. Retrieved from https://link.springer.com/article/10.1007/s12127-013-0122-8 

Wu, B., Wang, X., Zhao, B., & Bian, E. (2013). Electrophysiology properties of voltage-gated potassium channels in rat peritoneal macrophages. … journal of clinical and …. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609692/ 

Xu, J., Yu, P., Yang, Q., & Meng, J. (2013). Antiarrhythmic Efficacy of CPUY11018 Under Pathological Conditions. Drug Development …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1002/ddr.21060/full 

Zhang, G., Wei, H., Lu, J., Wong, P., & Shim, W. (2013). Identification and Characterization of Calcium Sparks in Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0055266 

Zhang, H. (2013). Dynamic effects of TNF-α on synaptic transmission in mice over time following sciatic nerve chronic constriction injury. Journal of …. Retrieved from https://jn.physiology.org/content/early/2013/07/12/jn.01088.2012.abstract 

Zhang, Y., Jia, Y., Guo, J., Liu, P., & Jiang, J. (2013). Effects of (−)-Gallocatechin-3-Gallate on Tetrodotoxin-Resistant Voltage-Gated Sodium Channels in Rat Dorsal Root Ganglion Neurons. International journal of …. Retrieved from https://www.mdpi.com/1422-0067/14/5/9779/pdf 

Zhang, Z., Matos, S., Jego, S., Adamantidis, A., & Séguéla, P. (2013). Norepinephrine Drives Persistent Activity in Prefrontal Cortex via Synergistic α1 and α2 Adrenoceptors. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0066122 

RelatedItems

P-30

P-30

Vertical MicroPipette Puller from Sutter

View details...

P-1000

P-1000

Next Generation Micropipette Puller from Sutter

View details...

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

Keep in Touch

We promise NEVER to share your details with anyone. You can opt out at any time.