CALBUF-2
Set of 11 Calcium Buffers
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Overview
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CALBUF-2 Instruction Manual
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- Use with calcium florescent indicators
- 20 mL of each buffer
Details
CALBUF-2 is especially suitable for calibrating fluorescent Ca++ indicators. It provides eleven buffer standards in the 10-4 to 10-8 M Ca++ range, whereas other commonly used fluorescent Ca++ indicators have the apparent Kd in the range of 100 to 300 nM. As with any ionic sensitive indicator, the sensitivity range of these indicators is about 1.0 log unit above and below the Kd. CALBUF-2 provides seven calibration points in this sensitivity range. It has an osmolarity of 0.305, which is isotonic with most mammalian cells.
Shelf life
Limited shelf life; use within 30 days.
Concentrations
1x10-8, 4x10-8, 1x10-7, 2.5x10-7, 5x10-7, 7.5x10-7, 1x10-6, 4x10-6, 1x10-5, 4x10-5, and 1x10-4 M at 20°C. Ionic strength: 0.150?M. 11 bottles, 20 mL each.
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