MCFX-400

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MCFX Datasheet
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Key Features

• Uniaxial stretch of 16 culture wells in a single-use silicone well plate
• 0.25mm thick membranes for high-magnification imaging using an inverted microscope
• PC-independent operation
• User-friendly interface software for specifying simple, cyclic, and intermittent stimulation protocols for download to the device controller

The MCFX uses a single-use flexible silicone well plate to culture cells in much the same environment as a traditional polystyrene well plate. After allowing the cells to develop adhesion to the well bottom, the MCFX can execute a user-specified stretch protocol to the well plate, causing the cells to deform. The well bottoms have similar optical properties to a glass coverslip, permitting high-magnification imaging of the cultured cells. The well plates can be sterilized and the system is suitable for long-term cell culture in a laboratory incubator.

Adhered Cell Monolayer Culture

Single-use silicone well plate.  Cell culture wells are 8mm X 8mm with a 0.25mm thick transparent bottom membrane. The 16 cell culture surfaces are shown in red.

This will be the feed from an external API...

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Tran, R.D., Siemens, M., Nguyen, C.H., Ochs, A.R., Zaragoza, M.V. and Grosberg, A. (2020). The Effect of Cyclic Strain on Human Fibroblasts With Lamin A/C Mutations and Its Relation to Heart Disease. Journal of biomechanical engineering vol. 142,6 (2020): 0610021–0610029. doi:10.1115/1.4044091

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