World Precision Instruments

Search the site by KEYWORD


Quick Finder


Testimonials

Researcher, ICBAS - Universidade do Porto

WPI provided a very professional, supportive and quick service upon purchasing several pieces of scientific equipment.
read more

BSK5

BSK5

Interface slice pre-incubator with two rings



Choose Quantity
  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

BSK5 BSK5 BSK5

There are 3 images available to view - click to enlarge and scroll through the product gallery.

BSK5 Brochure
/ Download as PDF

  • Slices maintained for many hours at an interface with incubating media
  • Two rings allow separation of different types of preparations
  • Slices supported on a quick change nylon net with optional attached porous memrane
  • Modular design simple to set up and maintain


The BSK5-2 Brain Slice Keeper has been designed to pre-incubate a large number of brain slices prior to transfer into recording chambers. Based on the BSK4, it consists of two closely fitting acrylic rings, located in a half cylinder manifold. The close fitting rings allow a removable sheet of nylon netting to be wedged and held in place. In this slice keeper, the slices are maintained at an interface between the incubation medium and a humidified, high oxygen atmosphere within the holding vessel. Furthermore the slices can be maintained on a flat porous membrane (eg *Millicell-cm, 0.4um Cat.# PICMORG50 from Millipore) attached to the nylon netting in order to maintain the underside of slices perfectly flat for subsequent experiments utilizing microscope techniques.

In operation, the manifolds carrying two rings each are located in the base of the holding vessel and are partially immersed with ACSF by filling to the level of the netting. The purpose-designed holding vessel is supplied with a 95% O2, 5% CO2 gas mixture via a ceramic air-stone. The air-stone is located to the side of the manifold in BSK5-2. The bubbles rising from the base saturate the ACSF and provide constant circulation of medium to the slices from the underside. Typically slices are placed on lens tissue which rests on the nylon nets or optionally on porous membrane discs attached to the netting. Bubbles are prevented from rising and being trapped under the slices by the half cylinder deflector, thereby ensuring a continuous circulation of medium. Slices remain viable for many hours in these conditions. The BSK5 together with the holding vessel can be easily placed into a water bath for regulating the incubating temperature as desired. A lid with a conical profile ensures that condensation drops do not fall directly on top of the slices. The typical fluid volume with BSK5-2 is around 250ml.

Schematic

Specifications

Accessories

Citations

RelatedItems

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

Keep in Touch

We promise NEVER to share your details with anyone. You can opt out at any time.