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Dr Gary Lawrence, Dublin City University

WPI are my preferred supplier for tissue and cell recording instrumentation. In my experience, WPI representatives have an excellent level of technical expertise and they have always responded in a timely manner with the most helpful, patient and instructive advice to aid the development of the o...
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FD35-100

FD35-100

FluoroDish, Glass Bottom, Clear Wall, 35 mm, 23 mm Well, Individually Packed, Sterile, 100/bx



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Overview

FD35-100

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FluoroDish Datasheet
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  • Optical quality glass bottom for better imaging quality
  • Low sample volume for expensive chemicals
  • Lowest access angle for micropipette
  • Low toxicity adhesive for embryo research

Measurements

  • ID: 33mm
  • OD: 35.5mm
  • Glass Diameter Φ: 23.5mm
  • height (inside): 7.8mm
  • Height (outside): 9mm
  • Access angle: 29 degrees

FluoroDish Schematic 

Exceptional Imaging Quality

WPI’s FluoroDish™ tissue culture dishes are now available in a larger range of sizes and coatings. These polycarbonate dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish, substantially better than some other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness stays within the 0.17 ±0.01 mm range.

Excellent for both classical and fluorescence microscopy

Each WPI dish has a flat (0.17 mm thick) optical quality glass bottom, allowing the use of a short objective working distance, large numerical aperture (NA), and a high magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in Image Analysis. The glass bottom permits the use of immersion objectives with medium such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

Materials

An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. All WPI dishes have the advantages of low toxicity and good UV transmission bottom glass. They are individually packed and gamma sterilized.

Features

The 35 mm dish has outside dimensions similar to that of a Corning 35 mm dish and has ø23.5 mm glass window. Most heaters and perfusion adapters designed for the Corning 35 mm dish will also fit this dish. Certain types of cell lines (e.g., PC3 and HEK) adhere well to the uncoated glass bottom dish. The users can also apply to the uncoated dish any special coating that is best for their cell line.

Human neural stem cells freshly dispersed by Accutase seeded on the glass coverslip bottom of a WPI Fluorodish, timelapse-imaged over a period of 6 days.

Specifications

Accessories

Citations

Roscioli, E., Germanova, T. E., Smith, C. A., Embacher, P. A., Erent, M., Thompson, A. I., … McAinsh, A. D. (2020). Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors. Cell Reports, 31(4). https://doi.org/10.1016/j.celrep.2020.107535

Forrester, A., Rathjen, S. J., Daniela Garcia-Castillo, M., Bachert, C., Couhert, A., Tepshi, L., … Johannes, L. (2020). Functional dissection of the retrograde Shiga toxin trafficking inhibitor Retro-2. Nature Chemical Biology, 16(3), 327–336. https://doi.org/10.1038/s41589-020-0474-4

Shah, A., Plaza-Sirvent, C., Weinert, S., Buchbinder, J. H., Lavrik, I. N., Mertens, P. R., … Lindquist, J. A. (2020). Yb-1 mediates tnf-induced pro-survival signaling by regulating nf-κb activation. Cancers, 12(8), 1–12. https://doi.org/10.3390/cancers12082188

Samassa, F., Ferrari, M. L., Husson, J., Mikhailova, A., Porat, Z., Sidaner, F., … Phalipon, A. (2020). Shigella impairs human T lymphocyte responsiveness by hijacking actin cytoskeleton dynamics and T cell receptor vesicular trafficking. Cellular Microbiology, 22(5). https://doi.org/10.1111/cmi.13166

Andersen, J. P., Zhang, J., Sun, H., Liu, X., Liu, J., Nie, J., & Shi, Y. (2020). Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport. Molecular Metabolism, 42, 101055. https://doi.org/10.1016/j.molmet.2020.101055

Mateus, R., Holtzer, L., Seum, C., Hadjivasiliou, Z., Dubois, M., Jülicher, F., & Gonzalez-Gaitan, M. (2020). BMP Signaling Gradient Scaling in the Zebrafish Pectoral Fin. Cell Reports, 30(12), 4292-4302.e7. https://doi.org/10.1016/j.celrep.2020.03.024

Ibrahim, A. F. M., Shen, L., Tatham, M. H., Dickerson, D., Prescott, A. R., Abidi, N., … Hay, R. T. (2020). Antibody RING-Mediated Destruction of Endogenous Proteins. Molecular Cell, 79(1), 155-166.e9. https://doi.org/10.1016/j.molcel.2020.04.032

Fore, S., Acuña-Hinrichsen, F., Mutlu, K. A., Bartoszek, E. M., Serneels, B., Faturos, N. G., … Yaksi, E. (2020). Functional properties of habenular neurons are determined by developmental stage and sequential neurogenesis. Science Advances, 6(36). https://doi.org/10.1126/sciadv.aaz3173

Alijevic, O., Bignucolo, O., Hichri, E., Peng, Z., Kucera, J. P., & Kellenberger, S. (2020). Slowing of the Time Course of Acidification Decreases the Acid-Sensing Ion Channel 1a Current Amplitude and Modulates Action Potential Firing in Neurons. Frontiers in Cellular Neuroscience, 14, 41. https://doi.org/10.3389/fncel.2020.00041

Van Der Meulen, K. L., Vöcking, O., Weaver, M. L., Meshram, N. N., & Famulski, J. K. (2020). Spatiotemporal Characterization of Anterior Segment Mesenchyme Heterogeneity During Zebrafish Ocular Anterior Segment Development. Frontiers in Cell and Developmental Biology, 8. https://doi.org/10.3389/fcell.2020.00379

Palumbo, F., Serneels, B., Pelgrims, R., & Yaksi, E. (2020). The Zebrafish Dorsolateral Habenula Is Required for Updating Learned Behaviors. Cell Reports, 32(8). https://doi.org/10.1016/j.celrep.2020.108054

Bolado-Carrancio, A., Rukhlenko, O. S., Nikonova, E., Tsyganov, M. A., Wheeler, A., Garcia-Munoz, A., … Kholodenko, B. N. (2020). Periodic propagating waves coordinate rhogtpase network dynamics at the leading and trailing edges during cell migration. ELife, 9, 1–34. https://doi.org/10.7554/eLife.58165

Ecke, M., Prassler, J., Tanribil, P., Müller-Taubenberger, A., Körber, S., Faix, J., & Gerisch, G. (2020). Formins specify membrane patterns generated by propagating actin waves. Molecular Biology of the Cell, 31(5), 373–385. https://doi.org/10.1091/mbc.E19-08-0460

Mulier, M., Van Ranst, N., Corthout, N., Munck, S., Vanden Berghe, P., Vriens, J., … Moilanen, L. (2020). Upregulation of TRPM3 in nociceptors innervating inflamed tissue. ELife, 9. https://doi.org/10.7554/eLife.61103

Rohani, L., Borys, B. S., Razian, G., Naghsh, P., Liu, S., Johnson, A. A., … Rancourt, D. E. (2020). Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01218-3

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Chronopoulos, A., Thorpe, S. D., Cortes, E., Lachowski, D., Rice, A. J., Mykuliak, V. V., … del Río Hernández, A. E. (2020). Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway. Nature Materials, 19(6), 669–678. https://doi.org/10.1038/s41563-019-0567-1

Beletkaia, E., Dashtbozorg, B., Jansen, R. G., Ruers, T. J. M., & Offerhaus, H. L. (2020). Nonlinear multispectral imaging for tumor delineation. Journal of Biomedical Optics, 25(09). https://doi.org/10.1117/1.jbo.25.9.096001

Ndao, O., Puech, P. H., Bérard, C., Limozin, L., Rabhi, S., Azas, N., … Dumètre, A. (2020). Dynamics of Toxoplasma gondii Oocyst Phagocytosis by Macrophages. Frontiers in Cellular and Infection Microbiology, 10. https://doi.org/10.3389/fcimb.2020.00207

Otis, J. P., & Farber, S. A. (2016). High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake. Journal of Visualized Experiments, (116), e54735–e54735. http://doi.org/10.3791/54735 

Arnold, W. D., Sheth, K. A., Wier, C. G., Kissel, J. T., Burghes, A. H., & Kolb, S. J. (2015). Electrophysiological Motor Unit Number Estimation (MUNE) Measuring Compound Muscle Action Potential (CMAP) in Mouse Hindlimb Muscles. Journal of Visualized Experiments, (103), e52899–e52899. http://doi.org/10.3791/52899 

Gindrat, A.-D., Quairiaux, C., Britz, J., Brunet, D., Lanz, F., Michel, C. M., & Rouiller, E. M. (2015). Whole-scalp EEG mapping of somatosensory evoked potentials in macaque monkeys. Brain Structure & Function, 220(4), 2121–42. http://doi.org/10.1007/s00429-014-0776-y 

Lee, E., Hong, J., Park, Y.-G., Chae, S., Kim, Y., Kim, D., … Sirota, A. (2015). Left brain cortical activity modulates stress effects on social behavior. Scientific Reports, 5, 13342. http://doi.org/10.1038/srep13342 

Nunes, P., Guido, D., & Demaurex, N. (2015). Measuring Phagosome pH by Ratiometric Fluorescence Microscopy. Journal of Visualized Experiments, (106), e53402–e53402. http://doi.org/10.3791/53402 

Pothoven, K. L., Norton, J. E., Hulse, K. E., Suh, L. A., Carter, R. G., Rocci, E., … Schleimer, R. P. (2015). Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Journal of Allergy and Clinical Immunology, 136(3), 737–746.e4. http://doi.org/10.1016/j.jaci.2015.01.043 

Rees, M. D., & Thomas, S. R. (2015). Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification. Journal of Visualized Experiments, (96), e52423–e52423. http://doi.org/10.3791/52423 

Srinivasan, B., Kolli, A. R., Esch, M. B., Abaci, H. E., Shuler, M. L., & Hickman, J. J. (2015). TEER measurement techniques for in vitro barrier model systems. Journal of Laboratory Automation, 20(2), 107–26. http://doi.org/10.1177/2211068214561025 

Steinritz, D., Schmidt, A., Balszuweit, F., Thiermann, H., Ibrahim, M., Bölck, B., & Bloch, W. (2015). Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals. Journal of Visualized Experiments, (101), e52768–e52768. http://doi.org/10.3791/52768 

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Blanchard, E., Zlock, L., Lao, A., Mika, D., Namkung, W., Xie, M., … Richter, W. (2014). Anchored PDE4 regulates chloride conductance in wild-type and ΔF508-CFTR human airway epithelia. FASEB Journal?: Official Publication of the Federation of American Societies for Experimental Biology, 28(2), 791–801. http://doi.org/10.1096/fj.13-240861 

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Perathoner, S., Daane, J. M., Henrion, U., Seebohm, G., Higdon, C. W., Johnson, S. L., … Levin, M. (2014). Bioelectric Signaling Regulates Size in Zebrafish Fins. PLoS Genetics, 10(1), e1004080. http://doi.org/10.1371/journal.pgen.1004080 

San Martín, A., Ceballo, S., Baeza-Lehnert, F., Lerchundi, R., Valdebenito, R., Contreras-Baeza, Y., … Barros, L. F. (2014). Imaging mitochondrial flux in single cells with a FRET sensor for pyruvate. PloS One, 9(1), e85780. http://doi.org/10.1371/journal.pone.0085780 

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Suntornsaratoon, P., Kraidith, K., Teerapornpuntakit, J., Dorkkam, N., Wongdee, K., Krishnamra, N., & Charoenphandhu, N. (2014). Pre-suckling calcium supplementation effectively prevents lactation-induced osteopenia in rats. American Journal of Physiology - Endocrinology and Metabolism, 306(2).

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Dalby-Brown, W., Jessen, C., Hougaard, C., Jensen, M. L., Jacobsen, T. A., Nielsen, K. S., … Jørgensen, S. (2013). Characterization of a novel high-potency positive modulator of Kv7 channels. European Journal of Pharmacology, 709(1), 52–63. http://doi.org/10.1016/j.ejphar.2013.03.039 

De Vos, A., Van de Velde, H., Bocken, G., Eylenbosch, G., Franceus, N., Meersdom, G., … Verheyen, G. (2013). Does intracytoplasmic morphologically selected sperm injection improve embryo development? A randomized sibling-oocyte study. Human Reproduction (Oxford, England), 28(3), 617–26. http://doi.org/10.1093/humrep/des435 

Dietmann, A., Millonig, A., Combes, V., Couraud, P.-O., Kachlany, S. C., & Grau, G. E. (2013). Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells. Microbial Pathogenesis, 6162, 43–50. http://doi.org/10.1016/j.micpath.2013.05.001 

Dietmann, A., Millonig, A., Combes, V., Couraud, P.-O., Kachlany, S. C., & Grau, G. E. (2013). Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells. Microbial Pathogenesis, 61, 43–50. http://doi.org/10.1016/j.micpath.2013.05.001 

Fernández, I. J., Gómez, P. N., Parodi, J., Mejía, F. R., & Salazar, R. S. (2013). Chilean crude extract of Ruta graveolens generates vasodilatation in rat aorta at subtoxic cellular concentrations. Advances in Bioscience and Biotechnology, 4(1), 29–36. http://doi.org/10.4236/abb.2013.41005 

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Furtado, J. M., Ashander, L. M., Mohs, K., Chipps, T. J., Appukuttan, B., Smith, J. R., … Chiu, F. (2013). Toxoplasma gondii Migration within and Infection of Human Retina. PLoS ONE, 8(2), e54358. http://doi.org/10.1371/journal.pone.0054358 

Geraldo, S., Simon, A., & Vignjevic, D. M. (2013). Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D. Journal of Visualized Experiments, (80), e50763–e50763. http://doi.org/10.3791/50763 

Hatanaka, Y., & Yamauchi, K. (2013). Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cerebral Cortex (New York, N.Y.?: 1991), 23(1), 105–13. http://doi.org/10.1093/cercor/bhr383 

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Hosny, N. A., Mohamedi, G., Rademeyer, P., Owen, J., Wu, Y., Tang, M.-X., … Kuimova, M. K. (2013). Mapping microbubble viscosity using fluorescence lifetime imaging of molecular rotors. Proceedings of the National Academy of Sciences of the United States of America, 110(23), 9225–30. http://doi.org/10.1073/pnas.1301479110 

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Tonurist, K., Thomberg, T., Janes, A., & Lust, E. (2013). Specific Performance of Electrical Double-Layer Capacitors Based on Different Separator Materials and Non-Aqueous Electrolytes. ECS Transactions, 50(43), 181–189. http://doi.org/10.1149/05043.0181ecst 

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Wang, J. T.-W., Berg, K., Høgset, A., Bown, S. G., & MacRobert, A. J. (2013). Photophysical and photobiological properties of a sulfonated chlorin photosensitiser TPCS(2a) for photochemical internalisation (PCI). Photochemical & Photobiological Sciences?: Official Journal of the European Photochemistry Association and the European Society for Photobiology, 12(3), 519–26. http://doi.org/10.1039/c2pp25328c 

Weinert, S., Poitz, D. M., Auffermann-Gretzinger, S., Eger, L., Herold, J., Medunjanin, S., … Braun-Dullaeus, R. C. (2013). The lysosomal transfer of LDL/cholesterol from macrophages into vascular smooth muscle cells induces their phenotypic alteration. Cardiovascular Research, 97(3), 544–52. http://doi.org/10.1093/cvr/cvs367 

Weinert, S., Poitz, D. M., Auffermann-Gretzinger, S., Eger, L., Herold, J., Medunjanin, S., … Braun-Dullaeus, R. C. (2013). The lysosomal transfer of LDL/cholesterol from macrophages into vascular smooth muscle cells induces their phenotypic alteration. Cardiovascular Research, 97(3). 

Yazejian, B., Yazejian, R. M., Einarsson, R., & Grinnell, A. D. (2013). Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures. Journal of Visualized Experiments, (73), e50253–e50253. http://doi.org/10.3791/50253 

Yin, B., Kuranov, R. V, McElroy, A. B., Kazmi, S., Dunn, A. K., Duong, T. Q., & Milner, T. E. (2013). Dual-wavelength photothermal optical coherence tomography for imaging microvasculature blood oxygen saturation. Journal of Biomedical Optics, 18(5), 56005. http://doi.org/10.1117/1.JBO.18.5.056005 

Yuseff, M. I., & Lennon-Dumenil, A. M. (2013). Studying MHC class II presentation of immobilized antigen by B lymphocytes. Methods in Molecular Biology (Clifton, N.J.), 960, 529–43. http://doi.org/10.1007/978-1-62703-218-6_39 

Zander, N. E., Orlicki, J. A., Rawlett, A. M., & Beebe, T. P. (2013). Electrospun polycaprolactone scaffolds with tailored porosity using two approaches for enhanced cellular infiltration. Journal of Materials Science: Materials in Medicine, 24(1), 179–187. http://doi.org/10.1007/s10856-012-4771-7 

Zhang, J., Jiang, D., Peng, H.-X., & Qin, F. (2013). Enhanced mechanical and electrical properties of carbon nanotube buckypaper by in situ cross-linking. Carbon, 63, 125–132. http://doi.org/10.1016/j.carbon.2013.06.047 

Zhu, Z., Sierra, A., Burnett, C. M.-L., Chen, B., Subbotina, E., Koganti, S. R. K., … Zingman, L. V. (2013). Sarcolemmal ATP-sensitive potassium channels modulate skeletal muscle function under low-intensity workloads. The Journal of General Physiology, 143(1).

Brenowitz, S. D., & Regehr, W. G. (2012). Presynaptic imaging of projection fibers by in vivo injection of dextran-conjugated calcium indicators. Cold Spring Harbor Protocols, 2012(4), 465–71. http://doi.org/10.1101/pdb.prot068551 

Goel, M., Sienkiewicz, A. E., Picciani, R., Wang, J., Lee, R. K., & Bhattacharya, S. K. (2012). Cochlin, Intraocular Pressure Regulation and Mechanosensing. PLoS ONE, 7(4), e34309. http://doi.org/10.1371/journal.pone.0034309 

Grama, A., & Engert, F. (2012). Direction selectivity in the larval zebrafish tectum is mediated by asymmetric inhibition. Frontiers in Neural Circuits, 6, 59. http://doi.org/10.3389/fncir.2012.00059 

Huber-Reggi, S. P., Chen, C.-C., Grimm, L., Straumann, D., Neuhauss, S. C. F., & Huang, M. Y.-Y. (2012). Severity of Infantile Nystagmus Syndrome-Like Ocular Motor Phenotype Is Linked to the Extent of the Underlying Optic Nerve Projection Defect in Zebrafish belladonna Mutant. Journal of Neuroscience, 32(50). 

Nakaya, N., Sultana, A., Lee, H.-S., & Tomarev, S. I. (2012). Olfactomedin 1 interacts with the Nogo A receptor complex to regulate axon growth. The Journal of Biological Chemistry, 287(44), 37171–84. http://doi.org/10.1074/jbc.M112.389916 

Owen, J., Zhou, B., Rademeyer, P., Tang, M.-X., Pankhurst, Q., Eckersley, R., & Stride, E. (2012). Understanding the structure and mechanism of formation of a new magnetic microbubble formulation. Theranostics, 2(12), 1127–39. http://doi.org/10.7150/thno.4307 

Seo, J., Yun, C.-O., Kwon, O.-J., Choi, E.-J., Song, J.-Y., Choi, I., & Cho, K.-H. (2012). A proteoliposome containing apolipoprotein A-I mutant (V156K) enhances rapid tumor regression activity of human origin oncolytic adenovirus in tumor-bearing zebrafish and mice. Molecules and Cells, 34(2), 143–8. http://doi.org/10.1007/s10059-012-2291-4 

Boccaccio, A., Sagheddu, C., & Menini, A. (2011). Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons. Journal of Visualized Experiments, (55), e3195–e3195. http://doi.org/10.3791/3195 

Cho, K.-H. (2011). Enhanced delivery of rapamycin by V156K-apoA-I high-density lipoprotein inhibits cellular proatherogenic effects and senescence and promotes tissue regeneration. The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences, 66(12), 1274–85. http://doi.org/10.1093/gerona/glr169 

Kizil, C., & Brand, M. (2011). Cerebroventricular microinjection (CVMI) into adult zebrafish brain is an efficient misexpression method for forebrain ventricular cells. PloS One, 6(11), e27395. http://doi.org/10.1371/journal.pone.0027395 

Li, W., Janardhan, A. H., Fedorov, V. V, Sha, Q., Schuessler, R. B., & Efimov, I. R. (2011). Low-energy multistage atrial defibrillation therapy terminates atrial fibrillation with less energy than a single shock. Circulation. Arrhythmia and Electrophysiology, 4(6), 917–25. http://doi.org/10.1161/CIRCEP.111.965830 

Lombardi, M. L., Zwerger, M., & Lammerding, J. (2011). Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation. Journal of Visualized Experiments, (55), e3087–e3087. http://doi.org/10.3791/3087 

Seo, J. H., Jang, I. K., Kim, H., Yang, M. S., Lee, J. E., Kim, H. E., … Cho, S.-R. (2011). Early Immunomodulation by Intravenously Transplanted Mesenchymal Stem Cells Promotes Functional Recovery in Spinal Cord Injured Rats. Cell Medicine, 2(2), 55–67. http://doi.org/10.3727/215517911X582788 

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Khuon, S., Liang, L., Dettman, R. W., Sporn, P. H. S., Wysolmerski, R. B., & Chew, T.-L. (2010). Myosin light chain kinase mediates transcellular intravasation of breast cancer cells through the underlying endothelial cells: a three-dimensional FRET study. Journal of Cell Science, 123(3), 431–440. http://doi.org/10.1242/jcs.053793 

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