AUTOLCI-BFRF4

Overview

Applications

• Wound Healing Assay
• Cell Confluency
• Co-Culture Monitoring
• Cell Migration
• Cell Proliferation
• Multi-Point Cell Monitoring
• Cell Morphology
• Cytoxicity Assay

Expand Your Cell Discoveries with Automated Live Cell Imaging

AutoLCI is an automated live cell imaging system that is equipped with an advanced fluorescence and bright field microscopy, autofocusing and real time multi-position imaging technology for a well plate, dish or T-flask.

The streamlined process provides an easy workflow solution giving you a full set of tools you need to acquire the best quality images and accurate research results.

The compact nature of the AutoLCI allows positioning in an incubator providing improved cell viability as there is less disturbances over the course of your experiment reducing chances of cellular abnormality.

The AutoLCI live cell imaging system includes two software packages

• AutoLCI Scan to control the actual operation of AutoLCI device which includes real-time cell monitoring and time-lapse imaging functions
• AutoLCI Analysis for analyzing and post-processing the images. This comes with an unlimited software usage license, ideal for allowing multiple users to work with the unit without additional fees

	Fluorescence imaging Bright field imaging and red fluorescence imaging
	Compact size AutoLCI is a live cell imaging system that easily fits into a standard CO2 incubator
	Multi-position imaging Fully-automated, multi-position imaging for high resolution analysis with a motorized camera that allows for multi-point imaging up to 96 wells.
	Autofocusing Increased focus speed and reproducibility with reliable autofocusing function
	High compatibility Compatible with various cell culture vessel types. Well plate (6, 12, 24, 48, 96 wells), Dish (35 mm, 60 mm, 90 mm), and T-flask (25 cm2 , 75cm2) may be selected.
	Intuitive interface With user-friendly functions, the easy-to-use analysis tools such as confluency mark, growth curve and a ruler are build into the included software.
	Z-stacking imaging Capture multiple focal planes and use the Z-stacking function to view high dynamic range (HDR) images.
	Image stitching Stitching combines images for analysis of a single high resolution composite image. This enables analysis of a larger volume and sections.

 

Unique Benefits of AutoLCI

Stable Stage – Get clearer images with a stable plate. Unlike other devices, AutoLCI has a fixed stage and the optics move.

Open-Source Data – Unlike other brands, AutoLCI gives you the raw images so that you can work with any analysis software you prefer, like Image J.

Regular Software Updates – Stay current with all the new functions of the AutoLCI software. Customers are lifetime members and are never charged for the software updates.

Unlimited Software Usage License – Multiple users may analyze images using their own computers (after the scanning is complete) without additional fees. Other brands limit usage to one computer.

Features

Multipoint imaging The optics system travels 117mm x 77mm, x and y axis respectively, multiple points within the travel range can be captured following the schedule (intervals, cycles, total time) set by the researcher. Different kinds of vessels can be used (Well plates, dishes, flasks, slides)
	Stable imaging performance AutoLCI doesn’t have a moveable stage but instead, the camera located inside the system moves to capture the images of cell in multiple positions Precise and sensitive fluorescence detection is possible with the integrated hard-coated optical set and LED filter with more than 50,000-hour lifetime.
Compact size AutoLCI is compact in size with 226(h) x 358(l) x 215(w)mm where several AutoLCI systems can fit into a standard CO2 incubator. Maintaining the performance of a device working in a hot and humid environment is very challenging. With AutoLCI, you can easily monitor live cells inside the incubator for a long time without disturbing the environment suitable for cell culture.

Scanning Application

The scanning application is used for capturing images. You can preview cells, schedule image capture, adjust light and contrast, and monitor time lapse progression from one intuitive screen. It includes auto-focusing technology that finds a clear focal plane of cells and has excellent repeatability.

Z-Stacking

With the Z-stacking function, where images of multiple planes of focus are merged, spheroid cells can be clearly observed under time-lapse imaging.

Image Stitching

Image stitching lets you capturing multiple images and combine the overlapping parts to enable high-resolution mapping of a large sample area.

Time Lapse Imaging

The AutoLCI Scan app gives you an intuitive interface for scheduling your time-lapse images. You can set the total time, cycle and intervals for the time lapse images of your live cell imaging setup.

 

Analysis Application

Auto LCI Analysis has a variety of tools in the analysis application simplify the analytic process, reducing errors and saving time.

• Intensity Histograms
• Confluency Graphs
• Channel Merge
• Video Creation
• Time Lapse Imaging
• Manual Counting
• Measurement
• Z-Stacking Image
• More

Specifications

Type	Description
Dimension	226 x 358 x 215 mm
Weight	5.6kg
Objective Lens	4X
Imaging modes	Bright field, Red Fluorescence
Fluorescence	Red: Excitation (510/84x) / Emission (570lp)
Light source	LED
Camera	5MP CMOS
Optics Stage	Motorized XYZ
Imaging positions	Multiple
File export format	TIFF, AVI, JPEG, PNG
Culture vessels	Well plate (6, 12, 24, 48, 96 wells), Dish* (35 mm, 60 mm, 90 mm), and T-flask* (25 cm2 , 75cm2)
Operating environment	10~40°C, 20~95% humidity
Power requirements	100-240V, ~50/60Hz
Output ports	Ethernet
Software**	AutoLCI Scan and AutoLCI Analysis
Accessories	PoE adapter, ethernet cable, USB/LAN adapter, USB memory
Warranty	1 year

* Requires optional adapters

** Requires External PC/Laptop with Windows 10 (not included with system). Recommended specs for PC/Laptop: Storage >1TB; Monitor: 1920 x 1080 pixels (Full HD)

Accessories

Citations

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