PV850

Features & Benefits

• High precision for reproducible injections – microliters to picoliter
• Touch screen display and control knob with intuitive user-interface
• Injection Counter
• Injection can be triggered manually using the touchscreen, by foot switch or by a computer-controlled TTL pulse
• Small footprint takes up very little bench space

The PV850 is designed to inject very small quantities of fluids, such as drugs, into cells or small organelles.Two different positive pressures may be applied – one for ejection at high pressure and a second, lower pressure to prevent back filling of the pipette by capillary action.

Applications

• Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
• Pre- and post-implantation in embryos of various species – mice, rats, monkeys, bovine, pigs, zebrafish, etc.

Overview

The PV850 requires an external pressure supply (not included) and is the ideal choice for applications that require serial experiments with a large number of injections.

Designed to simplify intracellular injection and a variety of other microinjection tasks, the PV850 uses carefully regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive “compensation” pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.

Timing, injection pressure and compensation pressure are adjusted independently using the touch screen interface and control knob. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch, manually, or a computer-controlled TTL pulse.

The PV850 offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically.

The display provides easy visibility of key data settings including Injector Pressure, Compensation Pressure, Injection Time, Pump Injection Mode and Injection Count