SYS-PV820

Overview

Discontinued - Newer Model Available

For comparision of the latest options

PV850 Microinjector with External Pressure Source (pressure source not included)

uPump Microinjector with Internal Pressure Source

• Regulated Hold and Ejection Pressure
• Carefully regulated air pressures for securing cells and injecting them with fluid
• Pressure Input: 0-150 psi
• Pressure Output: 0.3-90 psi

Intracellular injection

Repeatable microinjection in volumes ranging from picoliters to nanoliters. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid up­take through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.

PV820 Features

Separate regulated Hold and Ejection pressure, used to maintain a low pressure in the pipette between injections to prevent unwanted fluid uptake by capillary action or diffusion. A precision timing circuit switches from Eject pressure to Hold pressure automatically, once timing has been set. Although regulated vacuum is not provided in this model, suction can be provided by connecting a vacuum source to the vacuum port on the rear panel. Suction is then available through the pressure ports.

Ideal for zebrafish microinjection systems.

Specifications

PRESSURE

Pressure Input	0-150PSI
Pressure Output	0.3-90PSI
Lowest Regulated Pressure	re 12" water
Regulator Accuracy	0.1% (20-turn dial)
Regulator Repeatability	0.05PSI (both hold and eject pressures)
Gauge Accuracy	3% at full scale (both hold and eject pressures)
Input Connector	Quick Connect (1/4" OD tubing)
Output Connector	Barbed (1/16" ID tubing)
Control	Solenid
Pulse Width	10ms-10s in Timed Mode (10-turn dial)

VACUUM

Vacuum Input	0-30.0" Hg
Vacuum Output	Unregulated
Lowest Regulated Vacuum	Unregulated
Regulator Accuracy	Unregulated
Regulator repeatability	Unregulated
Guage Accuracy	None
Input Connector	Quick Connect (1/4" OD tubing)
Output Connector	Barbed (1/16" ID tubing)
Control	Manual
Vent	Atmosphere

 

CONNECTIONS INCLUDED

Input Kit	10' nylon tubing (0.25" OD, 1000PSI), one 1/4" female NPT adapter
Output Kit	Two PicoNozzle

 

PHYSICAL SPECS

Power	95-135V or 220-240V, 50/60Hz
Dimensions	17 x 3.5 x 9.5" (43 x 9 x 24cm)
Shipping Weight	11 lb. (5kg)

 

Accessories

Citations

Videos

JoVE Video: Konantz, J., Antos, C. L. "Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva." J. Vis. Exp. (88), e51595, doi:10.3791/51595 (2014). https://www.jove.com/video/51595/reverse-genetic-morpholino-approach-using-cardiac-ventricular.

JoVE Video: Kizil, C., Iltzsche, A., Kaslin, J., Brand, M. "Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides." J. Vis. Exp. (75), e50415, doi:10.3791/50415 (2013). https://www.jove.com/video/50415/micromanipulation-gene-expression-adult-zebrafish-brain-using

JoVE Video: Holmes, K. E., Wyatt, M. J., Shen, Y., Thompson, D. A., Barald, K. F. "Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development." J. Vis. Exp. (47), e2466, doi:10.3791/2466 (2011). https://www.jove.com/video/2466/direct-delivery-mif-morpholinos-into-zebrafish-otocyst-injection

JoVE Video: Cianciolo Cosentino, C., Roman, B. L., Drummond, I. A., Hukriede, N. A. "Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury." J. Vis. Exp. (42), e2079, doi:10.3791/2079 (2010). https://www.jove.com/video/2079/intravenous-microinjections-zebrafish-larvae-to-study-acute-kidney

JoVE Video: Russek-Blum, N., Nabel-Rosen, H., Levkowitz, G. "Two-Photon-Based Photoactivation in Live Zebrafish Embryos." J. Vis. Exp. (46), e1902, doi:10.3791/1902 (2010).

JoVE Video: Kague, E., Weber, C., Fisher, S. "Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences." J. Vis. Exp. (41), e1722, doi:10.3791/1722 (2010). https://www.jove.com/video/1902/two-photon-based-photoactivation-in-live-zebrafish-embryos

Papers

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Gonzalez, S., & Fernando, R. (2014). In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors. Nature protocols. Retrieved from https://www.nature.com/nprot/journal/v9/n5/abs/nprot.2014.073.html

Holt, J., Lane, S., & Jones, K. (2013). Time-Lapse Epifluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes. Mammalian Oocyte Regulation. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-191-2_6

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Kisielewska, J., & Whitaker, M. (2014). Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin In Vivo. Developmental Biology of the Sea Urchin and  …. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-974-1_15

Koga, S., Wüst, R., & Walsh, B. (2013). Increasing temperature speeds intracellular Po2 kinetics during contractions in single Xenopus skeletal muscle fibers. American Journal of …. Retrieved from https://ajpregu.physiology.org/content/304/1/R59.short

Kok, F., Gupta, A., Lawson, N., & Wolfe, S. (2014). Construction and application of site-specific artificial nucleases for targeted gene editing. Gene Function Analysis. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-721-1_13

Konantz, J., & Antos, C. (2014). Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva. JoVE (Journal of Visualized Experiments). Retrieved from https://www.jove.com/video/51595/reverse-genetic-morpholino-approach-using-cardiac-ventricular

Landry, T., & Fallon, J. (2013). Chronic neurotrophin delivery promotes ectopic neurite growth from the spiral ganglion of deafened cochleae without compromising the spatial selectivity ofcochlear. Journal of Comparative  …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1002/cne.23318/abstract

Li, X., Monckton, E., & Godbout, R. (2014). Ectopic expression of transcription factor AP-2δ in developing retina: effect on PSA-NCAM and axon routing. Journal of neurochemistry. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/jnc.12521/full

Llamas, M., & Sar, A. van der. (2014). Assessing Pseudomonas Virulence with Nonmammalian Host: Zebrafish. Pseudomonas Methods and Protocols. Retrieved from https://link.springer.com/protocol/10.1007/978-1-4939-0473-0_55

Luo, F., Guo, N., Li, S., Tang, H., Liu, Y., & Zhang, Y. (2014). Reduction of glutamate release probability and the number of releasable vesicles are required for suppression of glutamatergic transmission by β< sub> 1-. Neuropharmacology. Retrieved from https://www.sciencedirect.com/science/article/pii/S0028390814001257

Luo, F., Tang, H., Li, B., & Li, S. (2014). Activation of α1-adrenoceptors enhances excitatory synaptic transmission via a pre-and postsynaptic protein kinase C-dependent mechanism in the medial prefrontal. European Journal of Neuroscience. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/ejn.12495/full

Müller, I., & Melville, D. (2013). Functional modeling in zebrafish demonstrates that the atrial-fibrillation-associated gene GREM2 regulates cardiac laterality, cardiomyocyte differentiation and atrial. Disease models &  …. Retrieved from https://dmm.biologists.org/content/6/2/332.short

Nakashima, N., & Ishii, T. (2013). Hyperpolarisation-activated cyclic nucleotide-gated channels regulate the spontaneous firing rate of olfactory receptor neurons and affect glomerular formation in. The Journal of  …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1113/jphysiol.2012.247361/full

Park, K., Kim, J., & Cho, K. (2014). Elaidic acid (EA) generates dysfunctional high-density lipoproteins and consumption of EA exacerbates hyperlipidemia and fatty liver change in zebrafish. Molecular nutrition & food research. Retrieved from https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201300955/full

Park, K., Shin, D., & Cho, K. (2014). Dysfunctional Lipoproteins from Young Smokers Exacerbate Cellular Senescence and Atherogenesis with Smaller Particle Size and Severe Oxidation and Glycation. Toxicological Sciences. Retrieved from https://toxsci.oxfordjournals.org/content/early/2014/05/05/toxsci.kfu076.short

Ping, X., Jiang, K., & Lee, S. (2014). PEG-PDLLA Micelle Treatment Improves Axonal Function of the Corpus Callosum following Traumatic Brain Injury. Journal of  …. Retrieved from https://online.liebertpub.com/doi/abs/10.1089/neu.2013.3147

Schupp, M., Waas, M., Chun, C., & Ramchandran, R. (2014). Transcriptional inhibition of< i> etv2expression is essential for embryonic cardiac development. Developmental biology. Retrieved from https://www.sciencedirect.com/science/article/pii/S0012160614003157

Song, Y., Wang, M., Mao, F., & Shao, M. (2013). Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish. Disease Models &  …. Retrieved from https://dmm.biologists.org/content/6/2/404.short

Swann, K. (2013). Measuring Ca2+ Oscillations in Mammalian Eggs. Mammalian Oocyte Regulation. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-191-2_16

Tabor, K., & Bergeron, S. (2014). Direct activation of the Mauthner cell by electric field pulses drives ultra-rapid escape responses. Journal of  …. Retrieved from https://classic.jn.physiology.org/content/early/2014/05/16/jn.00228.2014.abstract

Takuwa, H., & Masamoto, K. (2013). Long-term adaptation of cerebral hemodynamic response to somatosensory stimulation during chronic hypoxia in awake mice. Journal of Cerebral  …. Retrieved from https://www.nature.com/jcbfm/journal/vaop/ncurrent/full/jcbfm201316a.html

Toth, P., Valota, A., & Velický, M. (2014). Electrochemistry in a drop: a study of the electrochemical behaviour of mechanically exfoliated graphene on photoresist coated silicon substrate. Chemical  …. Retrieved from https://pubs.rsc.org/en/content/articlehtml/2014/sc/c3sc52026a

Ueta, Y., Otsuka, T., & Morishima, M. (2013). Multiple Layer 5 Pyramidal Cell Subtypes Relay Cortical Feedback from Secondary to Primary Motor Areas in Rats. Cerebral  …. Retrieved from https://cercor.oxfordjournals.org/content/early/2013/04/02/cercor.bht088.short

Valota, A., Toth, P., Kim, Y., & Hong, B. (2013). Electrochemical Investigation of Chemical Vapour Deposition Monolayer and Bilayer Graphene on the Microscale. Electrochimica  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0013468613006269

Vatine, G., Zada, D., & Lerer-Goldshtein, T. (2013). Zebrafish as a model for monocarboxyl transporter 8-deficiency. Journal of Biological  …. Retrieved from https://www.jbc.org/content/288/1/169.short

Wagley, S., Hemsley, C., & Thomas, R. (2014). The Twin Arginine Translocation System Is Essential for Aerobic Growth and Full Virulence of Burkholderia thailandensis. Journal of  …. Retrieved from https://jb.asm.org/content/196/2/407.short

Zha, Y., Wang, Y., & Deng, Y. (2013). Exercise Training Lowers the Enhanced Tonically Active Glutamatergic Input to the Rostral Ventrolateral Medulla in Hypertensive Rats. CNS neuroscience & …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/cns.12065/full

Zhang, Y., & Kaneko, R. (2014). The vestibulo-and preposito-cerebellar cholinergic neurons of a ChAT-tdTomato transgenic rat exhibit heterogeneous firing properties and the expression of various. European Journal of  …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/ejn.12509/full

Zulian, A., Rizzo, E., & Schiavone, M. (2014). NIM811, a cyclophilin inhibitor without immunosuppressive activity, is beneficial in collagen VI congenital muscular dystrophy models. Human molecular  …. Retrieved from https://hmg.oxfordjournals.org/content/early/2014/05/22/hmg.ddu254.short

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